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Urological Research

, Volume 40, Issue 2, pp 143–150 | Cite as

Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin

  • Ashish Gupta
  • Mayank Dwivedi
  • Abbas Ali Mahdi
  • G. A. Nagana Gowda
  • Chunni Lal Khetrapal
  • Mahendra Bhandari
Original Paper

Abstract

Proanthocyanidin is commonly used for inhibiting urinary tract infection (UTI) of sensitive strains of Escherichia coli. The aim of this study was to investigate the effect of proanthocyanidin on adherence of uropathogenic multi-drug resistant E. coli to uroepithelial cells, which has not yet been investigated so far. Extracts of the purified proanthocyanidin were prepared from dried cranberry juice. Purity and structural assignment of proanthocyanidin was assessed using high performance liquid chromatography and 13C nuclear magnetic resonance spectroscopy, respectively. Subsequently, its affect on multi-drug resistant bacteria as well as quantification of anti-adherence bioactivity on human vaginal and bladder epithelial cells was appraised. Inhibition of adherence to an extent of about 70% with multi-drug resistant E. coli strains was observed on uroepithelial cell. The anti-adherence bioactivity of the proanthocyanidin was detected at concentrations of 10–50 µg/ml with significant bacteriuria. Probable proanthocyanidin through A-type linkages either combines to P-fimbriae of bacterial cells or modifies the structural entity of P-fimbriae and inhibits bacterial adherence to uroepithelial cells. The proanthocyanidin exhibited anti-adherence property with multi-drug resistant strains of uropathogenic P-fimbriated E. coli with in vitro study. Hence proanthocyanidin may be considered as an inhibitory agent for multi-drug resistant strains of E. coli adherence to uroepithelial cells.

Keywords

Proanthocyanidin Bacterial adherence Multi-drug resistance E. coli 

Abbreviations

NMR

Nuclear magnetic resonance

HPLC

High performance liquid chromatography

RBCs

Red blood cells

E. coli

Escherichia coli

VECs

Vaginal epithelial cells

BECs

Bladder epithelial cells

MF

McFarland

CFA

Colonization factor agar

AMC

Amoxicillin–Clavulanic Acid

UTI

Urinary tract infection

PBS

Phosphate buffer saline solution

Notes

Acknowledgments

We gratefully acknowledge the financial support from the Department of Science and Technology and council of scientific and industrial research, New Delhi, India.

Conflict of interest

None.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ashish Gupta
    • 1
  • Mayank Dwivedi
    • 2
  • Abbas Ali Mahdi
    • 3
  • G. A. Nagana Gowda
    • 1
  • Chunni Lal Khetrapal
    • 1
  • Mahendra Bhandari
    • 4
  1. 1.Centre of Biomedical Magnetic ResonanceSanjay Gandhi Postgraduate Institute of Medical SciencesLucknowIndia
  2. 2.Department of MicrobiologyLady Hardinge Medical CollegeNew DelhiIndia
  3. 3.Department of BiochemistryChhatrapati Shahuji Maharaj Medical UniversityLucknowIndia
  4. 4.Vattikuti Urology InstituteHenry Ford Hospital SystemDetroitUSA

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