Urological Research

, Volume 39, Issue 5, pp 379–383 | Cite as

Why does atorvastatin inhibit renal crystal retention?

  • Masao Tsujihata
  • Iwao Yoshioka
  • Akira Tsujimura
  • Norio Nonomura
  • Akihiko Okuyama
Original Paper


Recently, we reported that atorvastatin prevents renal tubular cell injury by oxalate and inhibits renal crystal retention. In this study, we investigated the mechanism by which atorvastatin inhibits renal crystal retention. Male Sprague-Dawley rats were separated into four experimental groups, and the ethylene glycol model of hyperoxaluria and the atorvastatin treatment model were analyzed. To clarify the mechanism by which atorvastatin inhibits renal crystal retention, the removed kidneys were used for the quantitative analysis of superoxide dismutase (SOD) and catalase. The subunits of the NADPH oxidase system were evaluated using real-time polymerase chain reaction analysis. Furthermore, the level of transforming growth factor-β (TGF-β) in kidney tissue was compared in each group. Atorvastatin treatment increased the SOD and catalase level compared with the stone-forming control group. Atorvastatin treatment decreased the expression of NOX-1 mRNA. Furthermore, the level of TGF-β was suppressed by atorvastatin treatment. We found that atorvastatin have inhibited calcium oxalate (CaOX) urolithiasis formation. We hypothesize that the mechanism of action of atorvastatin involves inhibiting TGF-β and NADPH oxidase, and increasing the SOD and catalase level. We believe that atorvastatin will be helpful in the treatment of CaOX urolithiasis.


Atorvastatin Calcium oxalate urolithiasis Rat SOD Catalase NADPH oxidase TGF-β 


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Masao Tsujihata
    • 1
  • Iwao Yoshioka
    • 1
  • Akira Tsujimura
    • 1
  • Norio Nonomura
    • 1
  • Akihiko Okuyama
    • 1
  1. 1.Department of UrologyOsaka University Graduate School of MedicineSuitaJapan

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