Urological Research

, Volume 32, Issue 5, pp 332–337

Comparative analysis of different apoptosis detection methods in human testicular cancer

  • Hans Ulrich Schmelz
  • Michael Abend
  • Matthias Port
  • Michael Schwerer
  • Ekkehard W. Hauck
  • Wolfgang Weidner
  • Christoph Sparwasser
Original Paper

DOI: 10.1007/s00240-004-0419-5

Cite this article as:
Schmelz, H.U., Abend, M., Port, M. et al. Urol Res (2004) 32: 332. doi:10.1007/s00240-004-0419-5

Abstract

In situ end-labeling (ISEL) of internucleosomal 3′ DNA strand breaks and the morphological proof of nuclear chromatin condensation are two widely used methods to investigate and quantify apoptosis. However, it is still unclear whether both processes are linked with each other and if quantifying apoptosis by both methods leads to comparable results. Therefore, internucleosomal DNA fragmentation and chromatin condensation were measured simultaneously on double-fluorescence-labeled sections of 62 testicular tumors (47 nonseminomatous tumors and 15 seminomas) using immunofluorescence microscopy. Different apoptotic indices (AI), based on DNA fragmentation and/or morphological criteria were determined. The AI were quantified. Morphologically obtained AI ranged between 1.99% for non-seminomatous tumors and 0.88% for seminomas. The detection of DNA fragmentation values ranged between 8.15% for non-seminomatous tumors and 2.70% for seminomas. Only about 30% of all apoptotic cells could be detected with the morphological method compared to 80% using ISEL in both tumor entities. Therefore, the equivalence of investigations using different apoptosis detection methods in human testicular cancer seems questionable.

Keywords

Apoptosis Testicular cancer DNA fragmentation Chromatin condensation Detection method 

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Hans Ulrich Schmelz
    • 1
    • 4
  • Michael Abend
    • 2
  • Matthias Port
    • 2
  • Michael Schwerer
    • 3
  • Ekkehard W. Hauck
    • 4
  • Wolfgang Weidner
    • 4
  • Christoph Sparwasser
    • 1
  1. 1.Department of UrologyFederal Armed Forced Hospital
  2. 2.Institute of RadiobiologyFederal Armed Forces Medical Academy
  3. 3.Department of PathologyFederal Armed Forced Hospital
  4. 4.Department of UrologyJustus Liebig University

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