Urological Research

, Volume 32, Issue 3, pp 219–222 | Cite as

Biochemical analysis of urethral collagen content after tubularized incised plate urethroplasty: an experimental study in rabbits

  • Fatma Taneli
  • Cevval Ulman
  • Abdulkadir Genc
  • Omer Yilmaz
  • Can Taneli
Original Paper

Abstract

The aim of the present study was the biochemical analysis of tissue hydroxyproline levels in incised urethral plates in order to show the total collagen content after the Snodgrass operation in the hypospadiac rabbit model. The study comprised 21 male New Zealand rabbits, (2.2–2.4 kg). The animals were randomly allocated to three groups each containing seven rabbits as follows: group 1, the ventral urethra was completely excised and a model of hypospadias formed. A full-thickness incision was made on the distal dorsal urethra, a feeding tube was placed as an urethral catheter and both urethral wings were sutured ventrally. Group 2, inserting an iris knife into the urethra, the ventral wall was incised mimicking an urethrotome. Group 3 consisted of normal control rabbits to determine the basal tissue hydroxyproline level. A slight increase in the hydroxyproline level was observed in the ventral part of the urethral tissue compared to the dorsal part in both groups 1 and 2; however, these differences were not significant. After the Snodgrass operation in the rabbit model, no significant differences were observed in the hydroxyproline levels of the dorsal and ventral parts of the urethra or between these and of the controls. Further studies are required in order to determine the mechanism underlying urethral healing through normal re-epithelization without excess collagen deposition after incised urethral plate urethroplasty.

Keywords

Hypospadias Snodgrass Urethra Hydroxyproline Collagen Scar 

References

  1. 1.
    Snodgrass W (1994) Tubularized, incised plate urethroplasty for distal hypospadias. J Urol 151: 464PubMedGoogle Scholar
  2. 2.
    Snodgrass W, Koyle M, Manzoni G, Hurwitz R, Caldamone A, Ehlich R (1998) Tubularized incised plate hypospadias repair for proximal hypospadias. J Urol 159: 2129PubMedGoogle Scholar
  3. 3.
    Lorenzo AJ, Snodgrass WT (2002) Regular dilatation is unnecessary after tabularized incised plate hypospadias repair. BJU Int 90: 473Google Scholar
  4. 4.
    Elbakry A (1999) Tubularized incised urethral plate urethroplasty: is regular dilatation necessary for success? BJU Int 84: 683CrossRefPubMedGoogle Scholar
  5. 5.
    Elbakry A (2002) Further experience with the tabularized incised urethral plate technique for hypospadias repair. BJU Int 89: 291CrossRefPubMedGoogle Scholar
  6. 6.
    Da-Silva EA, Sampaio FJB, Ortiz V, Cardoso LE (2002) Regional differences in the extracellular matrix of the human spongy urethra as evidenced by the composition of glycosaminoglycans. J Urol 167: 2183CrossRefPubMedGoogle Scholar
  7. 7.
    Woessner JF (1961) The determination of hydroxyproline in tissue and protein samples containing small proportions of this iminoacid. Arch Biochem Biophys 93: 440Google Scholar
  8. 8.
    Snodgrass W (1999) Does tubularized incised plate hypospadias repair create neourethral strictures? J Urol 162: 1159PubMedGoogle Scholar
  9. 9.
    Snodgrass W, Patterson K, Plaire JC, Grady R, Mitchell ME (2000) Histology of the urethral plate: implications for hypospadias repair. J Urol 64: 988CrossRefGoogle Scholar
  10. 10.
    Genc A, Taneli C, Gunsar C, Turkdogan P, Yilmaz O, Arslan OA, Mir E (2002) Histopathological evaluation of urethra after the Snodgrass operation: an experimental study in rabbits. BJU Int 90: 950CrossRefPubMedGoogle Scholar
  11. 11.
    Bleustein CB, Esposito MP, Soslow RA, Felsen D, Poppas DP (2001) Mechanism of healing following the Snodgrass repair. J Urol 165: 277PubMedGoogle Scholar
  12. 12.
    Lopes JF, Schned A, Ellsworth PI, Cendron M (2001) Histological analysis of urethral healing after tubularized incised plate urethroplasty. J Urol 166: 1014CrossRefPubMedGoogle Scholar
  13. 13.
    Baskin LS, Erol A, Li YW, Cunha GR (1998) Anatomical studies of hypospadias. J Urol 160: 1108CrossRefPubMedGoogle Scholar
  14. 14.
    Erol A, Baskin LS, Li YW, Liu WH (2000) Anatomical studies of the urethral plate: why preservation of the urethral plate is important in hypospadias repair. BJU Int 85: 728CrossRefPubMedGoogle Scholar
  15. 15.
    Kurzrock EA, Jegatheesan P, Cunha GR, Baskın LS (2000) Urethral development in the fetal rabbit and induction of hypospadias: a model for human development. J Urol 164: 1789Google Scholar
  16. 16.
    Barbagli G, Palminteri E, Lazzeri M, Guazzoni G, Turini D (2001) Long-term outcome of urethroplasty after failed urethrotomy versus primary repair. J Urol 165: 1918CrossRefPubMedGoogle Scholar
  17. 17.
    Baskin LS, Constantinescu SC, Howard PS, McAninch JW, Ewalt DH, Duckett JW, Snyder HM, Macarak EJ (1993) Biochemical characterization and quantitation of the collagenous components of urethral stricture tissue. J Urol 150: 642PubMedGoogle Scholar
  18. 18.
    Da-Silva EA, Sampaio FJ, Dornas MC, Damiao R, Cardoso LE (2002) Extracellular matrix changes in urethral stricture disease. J Urol 168: 805CrossRefPubMedGoogle Scholar
  19. 19.
    Sutherland RS, Baskin LS, Hayward SW, Cunha GR (1996) Regeneration of bladder urothelium, smooth muscle, blood vessels and nerves into an acellular tissue matrix. J Urol 156: 571PubMedGoogle Scholar
  20. 20.
    Kropp BP, Badylak S, Thor KB (1995) Regenerative bladder augmentation: a review of the initial preclinical studies with porcine small intestinal submucosa. Adv Exp Med Biol 385: 229PubMedGoogle Scholar
  21. 21.
    Baskin LS, Sutherland RS, Thomson AA, Nguyen HT, Morgan DM, Hayward SW, Hom YK, DiSandro M, Cunha GR (1997) Growth factors in bladder wound healing. J Urol 157: 2388CrossRefPubMedGoogle Scholar
  22. 22.
    Bindels EM, Van der Kwast TH, Izadifar V, Chopin DK, De Boer WI (2002) Functions of epidermal growth factor-like growth factors during human urothelial reepithelialization in vitro and the role of erbB2. Urol Res 30: 240CrossRefPubMedGoogle Scholar
  23. 23.
    Daher A, De Boer WI, El-Marjou A, Van der Kwast T, Abbou CC, Thiery JP, Radvanyi F, Chopin DK (2003) Epidermal growth factor receptor regulates normal urothelial regeneration. Lab Invest 83: 1333CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Fatma Taneli
    • 1
  • Cevval Ulman
    • 1
  • Abdulkadir Genc
    • 2
  • Omer Yilmaz
    • 2
  • Can Taneli
    • 2
  1. 1.Department of Clinical BiochemistryFaculty of Medicine
  2. 2.Pediatric SurgeryFaculty of Medicine

Personalised recommendations