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Urological Research

, Volume 30, Issue 5, pp 282–288 | Cite as

Involvement of mitochondrial pathway in Taxol-induced apoptosis of human T24 bladder cancer cells

  • Sheau-Yun Yuan
  • Shih-Lan Hsu
  • Kan-Jen Tsai
  • Chi-Rei Yang
Original Paper

Abstract.

We examined a human urothelial cancer T24 cell line, which was exposed to clinically achievable concentrations of Taxol and detected the lethal effect of Taxol as measured by a cytotoxic dose-response curve. Marked nuclear condensation and the fragmentation of chromatin were observed by DAPI stain, DNA ladder formation, and flow cytometry at an LC90 concentration of 0.8 µg/ml Taxol, which also induced a G2/M arrest. In response to Taxol-treatment, caspase-9 activity increased at 8 h, and both caspase-2 and -3 activities were increased twofold relative to control cultures at 16 h. Moreover, treatment with the broad-spectrum caspase inhibitor (z-VAD-fmk) or the caspase-9 specific inhibitor (z-LEHD-fmk) effectively protected T24 cells against Taxol-triggered apoptosis. Furthermore, the phosphorylation of Bcl-2 and Bcl-XL proteins in Taxol treated cells was detected at 8 h. In contrast, Taxol had no effect on the levels of Fas and FasL proteins and neither antagonistic, anti-Fas antibody affected Taxol-induced apoptosis. These results suggest that, following the phosphorylation of Bcl-2 and Bcl-XL proteins, Taxol-induced apoptosis is induced through the mitochondria-dependent pathway in T24 cells.

Taxol Urothelial cancer Apoptosis Bcl-2 Caspases 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Sheau-Yun Yuan
    • 1
  • Shih-Lan Hsu
    • 2
  • Kan-Jen Tsai
    • 3
  • Chi-Rei Yang
    • 1
  1. 1.Department of Surgery, Division of Urology, Taichung Veterans General Hospital, No 160, section 3, Chung-Gang Road, Taichung, Taiwan
  2. 2.Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan
  3. 3.School of Medicine, Chung Shan Medical University, Taichung, Taiwan

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