Journal of Molecular Evolution

, Volume 87, Issue 1, pp 7–15 | Cite as

Genomic Signatures Among Acanthamoeba polyphaga Entoorganisms Unveil Evidence of Coevolution

  • Víctor Serrano-SolísEmail author
  • Paulo Eduardo Toscano Soares
  • Sávio T. de Farías
Original Article


The definition of a genomic signature (GS) is “the total net response to selective pressure”. Recent isolation and sequencing of naturally occurring organisms, hereby named entoorganisms, within Acanthamoeba polyphaga, raised the hypothesis of a common genomic signature despite their diverse and unrelated evolutionary origin. Widely accepted and implemented tests for GS detection are oligonucleotide relative frequencies (OnRF) and relative codon usage (RCU) surveys. A common pattern and strong correlations were unveiled from OnRFs among A. polyphaga's Mimivirus and virophage Sputnik. RCU showed a common A-T bias at third codon position. We expanded tests to the amoebal mitochondrial genome and amoeba-resistant bacteria, achieving strikingly coherent results to the aforementioned viral analyses. The GSs in these entoorganisms of diverse evolutionary origin are coevolutionarily conserved within an intracellular environment that provides sanctuary for species of ecological and biomedical relevance.


Entoorganism Genomic Signature Virophage Mimivrus Acanthamoeba polyphaga Coevolution 



The authors thank Dr. Jonathas Abrahão for his valuable comments. This work was supported by a postdoctoral grant from PNPD/CAPES to VSS.

Author Contributions

VSS and STF conceived the project. VSS designed the experiments. VSS and PETS performed the experiments. VSS, STF and PETS analyzed the data. VSS and PETS wrote the paper.

Compliance with Ethical Standards

Competing interests

The authors declare no competing interests.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratório de Genética Evolutiva Paulo Leminsk, Departamento de Biologia Molecular, Centro de Ciencias Exatas e da NaturezaUniversidade Federal da ParaíbaJoão PessoaBrazil

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