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Journal of Molecular Evolution

, Volume 86, Issue 7, pp 431–442 | Cite as

Oligomerization of a Bimolecular Ribozyme Modestly Rescues its Structural Defects that Disturb Interdomain Assembly to Form the Catalytic Site

  • Md. Motiar Rahman
  • Shigeyoshi Matsumura
  • Yoshiya IkawaEmail author
Original Article

Abstract

The emergence of cellular compartmentalization was a crucial step in the hypothetical RNA world and its evolution because it would not only prevent the extinction of RNA self-replication systems due to dispersion/diffusion of their components but also facilitate ribozyme reactions by molecular crowding effects. Here, we proposed and examined self-assembly of RNA components as a primitive cellular-like environment, which may have the ability to mimic cellular compartmentalization and crowding effects. We engineered a bimolecular group I ribozyme to form a one-dimensional (1D)-ribozyme assembly. In the 1D assembly form, severe mutations that inactivated the parent bimolecular ribozyme were modestly rescued resulting in weak catalytic ability.

Keywords

Cellular compartmentalization Self-assembly Ribozyme RNA nanostructure RNA world 

Notes

Acknowledgements

This work was supported by MEXT KAKENHI Grant Number JP15K05561 (to Y.I.). This work was also supported partly by University of Toyama Discretionary Funds of the President “Toyama RNA Research Alliance” (to Y.I and S.M.).

Supplementary material

239_2018_9862_MOESM1_ESM.pdf (1.1 mb)
Supplementary material 1 (PDF 1127 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemistry, Graduate School of Science and EngineeringUniversity of ToyamaToyamaJapan
  2. 2.Graduate School of Innovative Life ScienceUniversity of ToyamaToyamaJapan

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