Journal of Molecular Evolution

, Volume 72, Issue 3, pp 265–273

Deleterious Mutations of a Claw Keratin in Multiple Taxa of Reptiles

  • Luisa Dalla Valle
  • Francesca Benato
  • Chiara Rossi
  • Lorenzo Alibardi
  • Erwin Tschachler
  • Leopold Eckhart
Article

DOI: 10.1007/s00239-010-9427-y

Cite this article as:
Dalla Valle, L., Benato, F., Rossi, C. et al. J Mol Evol (2011) 72: 265. doi:10.1007/s00239-010-9427-y

Abstract

We have recently shown that homologs of mammalian hair keratins are expressed in the claws of the green anole lizard, Anolis carolinensis. To test whether reptilian hair keratin homologs are functionally associated with claws, we investigated the conservation of the prototypical reptilian hair keratin homolog, hard acidic keratin 1 (HA1), in representative species from all main clades of reptiles. A complete cDNA of HA1 was cloned from the claw-forming epidermis of the lacertid lizard Podarcis sicula, and partial HA1 gene sequences could be amplified from genomic DNA of tuatara, lizards, gekkos, turtles, and crocodiles. In contrast, the HA1 gene of the limbless slow worm, Anguis fragilis, and of two species of turtles contained at least one deleterious mutation. Moreover, an HA1 gene was undetectable in the softshell turtle, snakes, and birds. Mapping the presence and absence of HA1 onto the phylogenetic tree of sauropsids suggested that the HA1 gene has been lost independently in several lineages of reptiles. The species distribution of HA1 is compatible with the hypothesis of a primary function of HA1 in claws but also shows that the formation of reptilian claws does not strictly depend on this keratin.

Keywords

Keratin Claw Reptiles Epidermis Gene loss Sauropsids Hair 

Supplementary material

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Supplementary material 1 (PDF 11 kb)
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Supplementary material 2 (PDF 18 kb)
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Supplementary material 3 (PDF 14 kb)
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Supplementary material 4 (PDF 15 kb)
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Supplementary material 5 (PDF 17 kb)
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Supplementary material 6 (PDF 17 kb)

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Luisa Dalla Valle
    • 1
  • Francesca Benato
    • 1
  • Chiara Rossi
    • 1
  • Lorenzo Alibardi
    • 2
  • Erwin Tschachler
    • 3
  • Leopold Eckhart
    • 3
  1. 1.Dipartimento di BiologiaUniversity of PadovaPadovaItaly
  2. 2.Dipartimento di BiologiaUniversity of BolognaBolognaItaly
  3. 3.Department of DermatologyMedical University of ViennaViennaAustria

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