Journal of Molecular Evolution

, Volume 68, Issue 3, pp 197–204 | Cite as

Removal of Noisy Characters from Chloroplast Genome-Scale Data Suggests Revision of Phylogenetic Placements of Amborella and Ceratophyllum

  • Vadim V. Goremykin
  • Roberto Viola
  • Frank H. Hellwig
Article

Abstract

It is widely appreciated that noisy, highly variable data can impede phylogeney reconstruction. Researchers have for a long time omitted problematic data from phylogenetic analyses, such as the third-codon positions and variable regions. In the analyses of the phylogenetic relations of the angiosperms; however, inclusion of complete gene sequences into genomic-scale alignments has become a common practice. Here we demonstrate that this practice can be misleading. We show that support of the basal-most position of Amborella trichopoda among the angiosperms in the chloroplast genomic data is based only on a tiny subset (< 1% of the total alignment length) of the most variable positions in alignment, exhibiting mean maximum likelihood (ML) distance among the angiosperm operational taxonomic units (OTUs) approximately 36 substitutions/site. Exclusion of these positions leads to disappearance of the basal Amborella branch. Likewise, the recently reported sister-group relationship of Ceratophyllum to the eudicots is based on the presence of 2% of the most variable positions in the genomic alignment, exhibiting, on average, 20 substitutions/site in comparison among the angiosperm OTUs. These observations highlight a need for excluding a certain proportion of saturated positions in alignment from phylogenomic analyses.

Keywords

Chloroplast genomes Molecular evolution Angiosperm diversification 

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Vadim V. Goremykin
    • 1
  • Roberto Viola
    • 1
  • Frank H. Hellwig
    • 2
  1. 1.IASMA Research CenterSan Michele all’AdigeItaly
  2. 2.Institut für Spezielle BotanikUniversität JenaJenaGermany

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