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Journal of Molecular Evolution

, Volume 65, Issue 3, pp 259–266 | Cite as

Patterns of Vertebrate Isochore Evolution Revealed by Comparison of Expressed Mammalian, Avian, and Crocodilian Genes

  • Jena L. ChojnowskiEmail author
  • James Franklin
  • Yoshinao Katsu
  • Taisen Iguchi
  • Louis J. GuilletteJr.
  • Rebecca T. Kimball
  • Edward L. Braun
Article

Abstract

Vertebrate genomes are mosaics of isochores, defined as long (>100 kb) regions with relatively homogeneous within-region base composition. Birds and mammals have more GC-rich isochores than amphibians and fish, and the GC-rich isochores of birds and mammals have been suggested to be an adaptation to homeothermy. If this hypothesis is correct, all poikilothermic (cold-blooded) vertebrates, including the nonavian reptiles, are expected to lack a GC-rich isochore structure. Previous studies using various methods to examine isochore structure in crocodilians, turtles, and squamates have led to different conclusions. We collected more than 6000 expressed sequence tags (ESTs) from the American alligator to overcome sample size limitations suggested to be the fundamental problem in the previous reptilian studies. The alligator ESTs were assembled and aligned with their human, mouse, chicken, and western clawed frog orthologs, resulting in 366 alignments. Analyses of third-codon-position GC content provided conclusive evidence that the poikilothermic alligator has GC-rich isochores, like homeothermic birds and mammals. We placed these results in a theoretical framework able to unify available models of isochore evolution. The data collected for this study allowed us to reject the models that explain the evolution of GC content using changes in body temperature associated with the transition from poikilothermy to homeothermy. Falsification of these models places fundamental constraints upon the plausible pathways for the evolution of isochores.

Keywords

Isochore Genome evolution Cytosine deamination GC content Homeothermy Poikilothermy 

Notes

Acknowledgments

We are grateful to the members of the Braun-Kimball lab group for helpful discussion and to Holly Kindsvater and Mike McCoy for help with R and statistical advice. This work was supported in part by grants to Y.K. and T.I. (Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan and grants from the Ministry of Environment, Japan) and to E.L.B. and L.J.G. (University of Florida Opportunity Fund).

Supplementary material

239_2007_9003_MOESM1_ESM.pdf (167 kb)
Supplementary material

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Jena L. Chojnowski
    • 1
    Email author
  • James Franklin
    • 1
  • Yoshinao Katsu
    • 2
    • 3
  • Taisen Iguchi
    • 2
    • 3
  • Louis J. GuilletteJr.
    • 1
  • Rebecca T. Kimball
    • 1
  • Edward L. Braun
    • 1
  1. 1.Department of ZoologyUniversity of FloridaFloridaUSA
  2. 2.Division of Molecular Environmental Endocrinology, Okazaki Institute for Integrative BioscienceNational Institute for Basic Biology, National Institutes of Natural SciencesOkazakiJapan
  3. 3.Department of Basic Biology, School of Life ScienceGraduate University for Advanced StudiesOkazakiJapan

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