Journal of Molecular Evolution

, Volume 64, Issue 3, pp 332–341 | Cite as

Ras-like Small GTPases Form a Large Family of Proteins in the Marine Sponge Suberites domuncula

  • Helena Cetkovic
  • Andreja Mikoc
  • Werner E. G. Müller
  • Vera Gamulin
Article

Abstract

Sponges (Porifera) are the simplest and the most ancient metazoan animals, which branched off first from the common ancestor of all multicellular animals. We have inspected ∼13,000 partial cDNA sequences (ESTs) from the marine sponge Suberites domuncula and have identified full or partial cDNA sequences coding for ∼50 different Ras-like small GTPases. Forty-four sponge proteins from the Ras family are described here: 6 proteins from the Ras subfamily, 5 from Rho, 6 from Arf, 1 Ran, and 26 Rabs or Rab-like proteins. No isoforms of these proteins were detected; the closest related proteins are two Rho proteins with 74% identity. Small GTPases from sponge display a higher degree of sequence conservation with orthologues from vertebrates (53%–93% identity) than with those from either Caenorhabditis elegans or Drosophila melanogaster. The real number of small GTPases in this sponge is certainly much higher than 50, because the actual S. domuncula database of ∼13,000 ESTs contains at most 3000 nonredundant cDNA sequences. The number of genes for Ras-like small GTPases in yeast, C. elegans, D. melanogaster, and humans is 30, 56, 90, and 174, respectively. Both model invertebrates have only 29 Rabs or Rab-like proteins, compared with 26 already found in sponge, and are missing at least 1 Rab (Rab24) present in S. domuncula and mammals. Our results indicate that duplications and diversifications of genes encoding Ras-like small GTPases, especially the Rab subfamily of small GTPases, happened very early in the evolution of Metazoa.

Keywords

Porifera Rab proteins Metazoan evolution Phagocytosis Axis formation 

Notes

Acknowledgments

We thank Matija Harcet for help with the deposition of sequences and S. Perovic-Ottstadt for performing in situ hybridization experiments. This work was supported by grants to V. Gamulin from the Croatian Ministry of Science, Technology and Education (MZOS-P0098072) and to W. E. G. Müller from the Deutsche Forschungsgemeinschaft (Mü/14-3).

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Helena Cetkovic
    • 1
  • Andreja Mikoc
    • 1
  • Werner E. G. Müller
    • 2
  • Vera Gamulin
    • 1
  1. 1.Department of Molecular BiologyRudjer Boskovic InstituteZagrebCroatia
  2. 2.Institut für Physiologische Chemie, Abteilung Angewandte MolekularbiologieJohannes Gutenberg UniversitätMainzGermany

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