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Journal of Molecular Evolution

, Volume 63, Issue 2, pp 208–221 | Cite as

Gene Duplications and Evolution of Vertebrate Voltage-Gated Sodium Channels

  • Alicia E. Novak
  • Manda C. Jost
  • Ying Lu
  • Alison D. Taylor
  • Harold H. Zakon
  • Angeles B. RiberaEmail author
Article

Abstract

Voltage-gated sodium channels underlie action potential generation in excitable tissue. To establish the evolutionary mechanisms that shaped the vertebrate sodium channel α-subunit (SCNA) gene family and their encoded Nav1 proteins, we identified all SCNA genes in several teleost species. Molecular cloning revealed that teleosts have eight SCNA genes, compared to ten in another vertebrate lineage, mammals. Prior phylogenetic analyses have indicated that the genomes of both teleosts and tetrapods contain four monophyletic groups of SCNA genes, and that tandem duplications expanded the number of genes in two of the four mammalian groups. However, the number of genes in each group varies between teleosts and tetrapods, suggesting different evolutionary histories in the two vertebrate lineages. Our findings from phylogenetic analysis and chromosomal mapping of Danio rerio genes indicate that tandem duplications are an unlikely mechanism for generation of the extant teleost SCNA genes. Instead, analyses of other closely mapped genes in D. rerio as well as of SCNA genes from several teleost species all support the hypothesis that a whole-genome duplication was involved in expansion of the SCNA gene family in teleosts. Interestingly, despite their different evolutionary histories, mRNA analyses demonstrated a conservation of expression patterns for SCNA orthologues in teleosts and tetrapods, suggesting functional conservation.

Keywords

Voltage-gated sodium channel Teleosts Gene families Genome duplication Gene duplication 

Notes

Acknowledgments

The authors’ work was supported by NIH grants (NS 38937—A.E.N., A.D.T., and A.B.R.; NS 25513—H.H.Z. and Y.L.; and NSF IBN 0236147—M.C.J.).

Supplementary material

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Supplementary material

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Alicia E. Novak
    • 1
  • Manda C. Jost
    • 2
    • 3
  • Ying Lu
    • 2
  • Alison D. Taylor
    • 1
  • Harold H. Zakon
    • 2
    • 4
  • Angeles B. Ribera
    • 1
    Email author
  1. 1.Department of Physiology and Biophysics, Mail Stop 8307, RC-1NUniversity of Colorado at Denver and Health Sciences CenterAuroraUSA
  2. 2.Section of NeurobiologyUniversity of Texas at AustinAustinUSA
  3. 3.Section of Integrative BiologyUniversity of Texas at AustinAustinUSA
  4. 4.The Josephine Bay Paul Center in Comparative and Molecular Biology and EvolutionMarine Biological LaboratoryWoods HoleUSA

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