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Molecular Basis of the Size Polymorphism of the First Intron of theAdh-1 Gene of the Mediterranean Fruit Fly, Ceratitis capitata

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Abstract

The first intron of the gene encoding one of the alcohol dehydrogenase isoenzymes (ADH-1) in Ceratitis capitata is highly polymorphic in size. Five size variants of this intron were isolated from different strains and populations and characterized. Restriction map and sequence analysis showed that the intron size polymorphism is due to the presence or absence of (a) a copy of a defective mariner-like element, postdoc; (b) an ∼550-bp 3 ′ indel which exhibits no similarity to any known sequence; and (c) a central duplication of 704 bp consisting of part of the 3′ end of the postdoc element, the region between postdoc and the 3 ′ indel, and the first 20 bp of the 3 ′ indel. The homologous Adh-1 intron was amplified from the congeneric species, Ceratitis rosa, in order to obtain an outgroup for comparative and phylogenetic analyses. The C. rosa introns were polymorphic in size, ranging from about 1100 to 2000 bp, the major difference between them being the presence or absence of a mariner-like element Crmar2, unrelated to the postdoc element. Phylogenetic analysis suggests that the shorter intron variants in C. capitata may represent the ancestral form of the intron, the longest variants apparently being the most recent.

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Acknowledgments

This work was supported by grants from the Greek Secretariat General for Research and Technology to C.S., a European Union Training Grant to S.B., and the Italian Ministry of Education, University & Research (MIUR) to G.G.

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  1. Saverio Brogna

    Present address: Molecular Cell Biology, School of Biosciences, University of Birmingham, B15 2TT, Birmingham, UK

Authors and Affiliations

  1. Department of Animal Biology, University of Pavia, Piazza Botta 9, I27100, Pavia, Italy

    Ludvik M. Gomulski & Giuliano Gasperi

  2. Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete, Greece

    Saverio Brogna, Alekos Babaratsas, Charalambos Savakis & Kostas Bourtzis

  3. Department of Biology, University of Patras, Patras, Greece

    Antigoni Zacharopoulou

  4. Division of Basic Sciences, Medical School, University of Crete, Heraklion, Crete, Greece

    Charalambos Savakis

  5. Department of Environmental and Natural Resources Management, University of Ioannina, 2 Seferi St, 30100, Agrinio, Greece

    Kostas Bourtzis

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  1. Ludvik M. Gomulski
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Gomulski, L.M., Brogna, S., Babaratsas, A. et al. Molecular Basis of the Size Polymorphism of the First Intron of theAdh-1 Gene of the Mediterranean Fruit Fly, Ceratitis capitata . J Mol Evol 58, 732–742 (2004). https://doi.org/10.1007/s00239-004-2596-9

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