The Promise and Peril of Continuous In Vitro Evolution
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Experimental evolution methods can be used to address and illuminate issues central to the understanding of evolutionary theory. One of the most powerful of these methods involves the in vitro evolution of nucleic acid enzymes, taking advantage of the direct relationship between the genotype of a nucleic acid sequence and the phenotype of its associated catalytic function. This review and commentary focuses on the past, present, and future potential of systems for the continuous in vitro evolution of nucleic acid enzymes as tools for modeling evolutionary processes in biology. It offers a candid appraisal of both the strengths and the limitations of these systems.
KeywordsContinuous evolution In vitro evolution Isothermal amplification Ligase Mutation Ribozyme Selection
This work was supported by Grant NAG5-9386 from the National Aeronautics and Space Administration and The Skaggs Institute for Chemical Biology.
- Gould, SJ, Vrba, ES 1982Exaptation—A missing term in the science of formPaleobiology8415Google Scholar
- Hill CS (1996) Gen-Probe transcription mediated amplification: system principles. Gen-Probe Inc. technical document. Available at: http://www.gen-probe.com/pdfs/tma_whiteppr.pdf
- Saiki, RK, Scharf, S, Faloona, F, Mullis, KB, Horn, GT, Erlich, HA, Arnheim, N 1985Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemiaScience 2301350–1354Google Scholar
- Wright, S 1931Evolution in Mendelian populationsGenetics1697159Google Scholar
- Wright, S 1932The roles of mutation, inbreeding, crossbreeding, and selection in evolutionProc 6th Int Congr Genet1356366Google Scholar