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Journal of Molecular Evolution

, Volume 59, Issue 6, pp 792–805 | Cite as

Evolutionary Diversity of Vertebrate Small Heat Shock Proteins

  • Erik Franck
  • Ole Madsen
  • Teun van Rheede
  • Guénola Ricard
  • Martijn A. Huynen
  • Wilfried W. de JongEmail author
Article

Abstract

All vertebrates express multiple small heat shock proteins (sHsps), which are important components of the cellular chaperoning machinery and display a spectacular diversity of functions. This ranges from remodeling the cytoskeleton and inhibiting apoptosis to serving as structural proteins in eye lens and sperm tail. Most information is available for the 10 known mammalian sHsps, formally named HspB1–B10. Only three of them (Hsp27/B1, αA-crystallin/B4, αB-crystallin/B5) have been reported from nonmammalian vertebrates, while an apparent paralog, Hsp30/B11, is found in frogs and teleost fish. To reconstruct the evolutionary diversification of the sHsps in vertebrates, we searched for additional sHsps in genome, protein, and EST databases and sequenced some avian and amphibian sHsps (HspB2, Hsp30/B11). The urochordate Ciona intestinalis was included in the search, as the outgroup of vertebrates. Orthologs of seven mammalian sHsps were now found in other vertebrate classes. Two novel sHsps, named HspB11 and HspB12, were recognized in birds, and four novel sHsps, named HspB12–B15, in teleost fish. Secondary structure predictions of orthologous sHsps from different vertebrate classes indicate conservation of the β-sandwich structure of the functionally important C-terminal “α-crystallin domain,” while the N-terminal domains generally have α-helical structures, despite their pronounced sequence variation. The constructed chordate sHsp tree is supported by shared introns, indels, and diagnostic sequences. The tree distinguishes putative orthologous and paralogous relationships, which will facilitate the functional and structural comparison of the various vertebrate sHsps. The 15 recognized paralogous vertebrate sHsps reflect the period of extensive gene duplications early in vertebrate evolution. Eleven of these sHsps are grouped in a clade that might be specific for chordates. It is inferred that at least 13 intron insertions have occurred during the evolution of chordate sHsp genes, while a single ancient intron is maintained in some lineages, in line with the general trend of massive intron gain before or during early vertebrate radiation. Interesting is the occurrence of several head-to-head located pairs of chordate sHsp genes.

Keywords

α-Crystallin Phylogeny Genome duplication Intron evolution Head-to-head genes Small heat shock proteins Moonlighting 

Notes

Acknowledgments

We thank Akie Sato and Jan Klein (Tubingen) for providing the EST of B. lanceolatum BlHsp24.1, Remco Rexwinkel for sequencing this sHsp, Guido Kappé for useful discussions, and the Netherlands Organization for Scientific Research (NWO-ALW) for financial support.

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Copyright information

© Springer Science + Business Media Inc. 2004

Authors and Affiliations

  • Erik Franck
    • 1
  • Ole Madsen
    • 1
  • Teun van Rheede
    • 1
  • Guénola Ricard
    • 2
  • Martijn A. Huynen
    • 2
  • Wilfried W. de Jong
    • 1
    Email author
  1. 1.Department of BiochemistryUniversity of NijmegenNijmegenThe Netherlands
  2. 2.Nijmegen Centre for Molecular Life Sciences, CMBlUniversity of NijmegenNijmegenThe Netherlands

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