Journal of Molecular Evolution

, Volume 58, Issue 2, pp 191–195 | Cite as

Exponential Spread of Hepatitis C Virus Genotype 4a in Egypt

  • Yasuhito Tanaka
  • Salah Agha
  • Niveen Saudy
  • Fuat Kurbanov
  • Etsuro Orito
  • Takanobu Kato
  • Mostafa Abo-Zeid
  • Mohamed Khalaf
  • Yuzo Miyakawa
  • Masashi Mizokami
Article

Abstract

Hepatitis C virus (HCV) infects >10% of the general population in Egypt, in which intravenous injection with an antimony compound for endemic schistosomiasis in the past has been implicated. To simulate the epidemic history of HCV in Egypt, sera were obtained from 3608 blood donors at 13 governorates in or surrounding the Nile valley during 1999. The prevalence of antibody to HCV (anti-HCV) and genotypes was determined in them, and the molecular evolutionary analysis based on the neutral theory was applied to HCV isolates of genotype 4a, which is outstandingly prevalent in Egypt and indigenous there. Of 3608 sera, 317 (8.8%) were positive for anti-HCV. The molecular evolutionary analysis on 47 HCV genotype 4a isolates of carriers from various districts in Egypt indicated that the spread of HCV-4a would have increased exponentially during the 1940s through 1980 when oral medications became available. In conclusion, the estimated spread time is consistent with the duration of intravenous antimony campaigns in Egypt.

Keywords

Hepatic C virus Genotype 4a Egypt Neutral theory Molecular evolutionary analyses Schistosomiasis Parenteral treatment 

References

  1. 1.
    Darwish, MA, Raouf, TA, Rushdy, P, Constantine, NT, Rao, MR, Edelman, R 1993Risk factors associated with a high seroprevalence of hepatitis C virus infection in Egyptian blood donors.Am J Trop Med Hyg49440447PubMedGoogle Scholar
  2. 2.
    Dusheiko, G, Schmilovitz-Weiss, H, Brown, D, McOmish, F, Yap, PL, Sherlock, S, Mclntyre, N, Simmonds, P 1994Hepatitis C virus genotypes: An investigation of type-specific differences in geographic origin and disease.Hepatology191318PubMedGoogle Scholar
  3. 3.
    Frank, C, Mohamed, MK, Strickland, GT, Lavanchy, D, Arthur, RR, Magder, LS, El Khoby, T, Abdel-Wahab, Y, Aly Ohn, ES, Anwar, W, Sallam, I 2000The role of parenteral antischistosomal therapy in the spread of hepatitis C virus in Egypt.Lancet355887891CrossRefPubMedGoogle Scholar
  4. 4.
    Hibbs, RG, Corwin, AL, Hassan, NF, Kamel, M, Darwish, M, Edelman, R, Constantine, NT, Rao, MR, Khalifa, AS, Mokhtar, S 1993The epidemiology of antibody to hepatitis C in Egypt.J Infect Dis168789790PubMedGoogle Scholar
  5. 5.
    Kamel, MA, Ghaffar, YA, Wasef, MA, Wright, M, Clark, LC, Miller, FD 1992High HCV prevalence in Egyptian blood donors.Lancet340427CrossRefGoogle Scholar
  6. 6.
    McOmish, F, Yap, PL, Dow, BC, Follett, EA, Seed, C, Keller, AJ, Cobain, TJ, Krusius, T, Kolho, E, Naukkarinen, R 1994Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey.J Clin Microbiol32884892PubMedGoogle Scholar
  7. 7.
    Nafeh, MA, Medhat, A, Shehata, M, Mikhail, NN, Swifee, Y, Abdel-Hamid, M, Watts, S, Fix, AD, Strickland, GT, Anwar, W, Sallam, I 2000Hepatitis C in a community in Upper Egypt: I. Cross-sectional survey.Am J Trop Med Hyg63236241PubMedGoogle Scholar
  8. 8.
    Ohno, T, Mizokami, M, Wu, RR, Saleh, MG, Ohba, K, Orito, E, Mukaide, M, Williams, R, Lau, JY 1997New hepatitis C virus (HCV) genotyping system that allows for identification of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a.J Clin Microbiol35201207PubMedGoogle Scholar
  9. 9.
    Pybus, OG, Rambaut, A 2002GENIE: Estimating demographic history from molecular phylogenies.Bioinformatics1814041405CrossRefPubMedGoogle Scholar
  10. 10.
    Pybus, OG, Rambaut, A, Harvey, PH 2000An integrated framework for the inference of viral population history from reconstructed genealogies.Genetics15514291437PubMedGoogle Scholar
  11. 11.
    Pybus, OG, Charleston, MA, Gupta, S, Rambaut, A, Holmes, EC, Harvey, PH 2001The epidemic behavior of the hepatitis C virus.Science29223232325CrossRefPubMedGoogle Scholar
  12. 12.
    Pybus, OG, Drummond, AJ, Nakano, T, Robertson, BH, Rambaut, A 2003The epidemiology and iatrogenic transmission of hepatitis C virus in egypt: a bayesian coalescent approach.Mol Biol Evol20381387CrossRefPubMedGoogle Scholar
  13. 13.
    Rao, MR, Naficy, AB, Schisterman, E, Clemens, JD, Edelman, R, Darwish, MA, Darwish, NM 2002Further evidence for association of hepatitis C infection with parenteral schistosomiasis treatment in Egypt.BMC Infect Dis229CrossRefPubMedGoogle Scholar
  14. 14.
    Ray, SC, Arthur, RR, Carella, A, Bukh, J, Thomas, DL 2000Genetic epidemiology of hepatitis C virus throughout egypt.J Infect Dis182698707CrossRefPubMedGoogle Scholar
  15. 15.
    Stumpf, MP, Pybus, OG 2002Genetic diversity and models of viral evolution for the hepatitis C virus.FEMS Microbiol Lett214143152CrossRefPubMedGoogle Scholar
  16. 16.
    Takeuchi, T, Katsume, A, Tanaka, T, Abe, A, Inoue, K, Tsukiyama-Kohara, K, Kawaguchi, R, Tanaka, S, Kohara, M 1999Real-time detection system for quantification of hepatitis C virus genome.Gastroenterology116636642PubMedGoogle Scholar
  17. 17.
    Tanaka, Y, Hanada, K, Mizokami, M, Yeo, AE, Shih, JW, Gojobori, T, Alter, HJ 2002Inaugural Article: A comparison of the molecular clock of hepatitis C virus in the United States and Japan predicts that hepatocellular carcinoma incidence in the United States will increase over the next two decades.Proc Natl Acad Sci USA991558415589CrossRefPubMedGoogle Scholar
  18. 18.
    WHO1993The control of schistosomiasis: Second report of the WHO expert committee (WHO Technical Report Series 830).WHOGenevaGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 2004

Authors and Affiliations

  • Yasuhito Tanaka
    • 1
  • Salah Agha
    • 3
  • Niveen Saudy
    • 3
  • Fuat Kurbanov
    • 1
  • Etsuro Orito
    • 2
  • Takanobu Kato
    • 1
  • Mostafa Abo-Zeid
    • 4
  • Mohamed Khalaf
    • 5
  • Yuzo Miyakawa
    • 6
  • Masashi Mizokami
    • 1
  1. 1.Department of Clinical Molecular Informative MedicineNagoya City University Graduate School of Medical Science, Kawasumi, Mizuho, Nagoya 467-8601Japan
  2. 2.Department of Internal Medicine and Molecular ScienceNagoya City University Graduate School of Medical Sciences, NagoyaJapan
  3. 3.Department of Clinical PathologyFaculty of Medicine, Mansoura University, MansouraEgypt
  4. 4.Department of SurgeryFaculty of Medicine, Mansoura University, MansouraEgypt
  5. 5.Department of Clinical PathologyFaculty of Medicine, Assuit University, AssuitEgypt
  6. 6.Miyakawa Memorial Research Foundation, TokyoJapan

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