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Journal of Molecular Evolution

, Volume 57, Issue 5, pp 555–561 | Cite as

A Novel Family of Unconventional Actins in Volvocalean Algae

  • Takako Kato-MinouraEmail author
  • Masayo Okumura
  • Masafumi Hirono
  • Ritsu Kamiya
Article

Abstract

The unicellular green alga Chlamydomonas reinhardtii has two actin genes, one encoding a conventional actin (90% amino acid identity with mammalian actin), the other a highly divergent actin (64% identity) named novel actin-like protein (NAP). To see whether the presence of conventional and unconventional actins in a single organism is unique to C. reinhardtii, we searched for genomic sequences related to the NAP sequence in several other species of volvocalean algae. Here we show that Chlamydomonas moewusii and Volvox carteri also have, in addition to a conventional actin, an unconventional actin similar to the C. reinhardtii NAP. Analyses of the deduced protein sequences indicated that the NAP homologues form a distinct group derived from conventional actin.

Keywords

Chlamydomonas Phylogeny Volvocales 

Notes

Acknowledgements

We acknowledge Drs. Tomoaki Nishiyama and Mitsuyasu Hasebe (National Institute for Basic Biology) for their kind help in the phylogenetic analyses and Drs. Takeshi Nakayama and Junichi Miyazaki (University of Tsukuba) for critical reading of the manuscript. The computations were partly performed on an SGI Origin 2000 in the NIBB Computer Laboratory and a SUN workstation (Sakura) in the Science Information Processing Center, University of Tsukuba. This work was supported by Grants-in-Aid for Encouragement of Young Scientists from Japan Society for the Promotion of Science, Japan, to T.K.-M.

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Copyright information

© Springer-Verlag New York Inc. 2003

Authors and Affiliations

  • Takako Kato-Minoura
    • 1
    • 2
    Email author
  • Masayo Okumura
    • 3
  • Masafumi Hirono
    • 4
  • Ritsu Kamiya
    • 1
    • 4
  1. 1.Department of Cell BiologyNational Institute for Basic Biology, Okazaki 444-8585Japan
  2. 2.Institute of Biological SciencesUniversity of Tsukuba, Tsukuba 305-8572Japan
  3. 3.Unit of BiosystemsGraduate School of Human Informatics, Nagoya University, Nagoya 464-8601Japan
  4. 4.Department of Biological SciencesGraduate School of Science, University of Tokyo, Tokyo 113-0033Japan

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