Disease stage-dependent relationship between diffusion tensor imaging and electrophysiology of the visual system in a murine model of multiple sclerosis

  • Christopher Nishioka
  • Hsiao-Fang Liang
  • Chen-Fang Chung
  • Shu-Wei Sun
Functional Neuroradiology

Abstract

Purpose

Diffusion tensor imaging (DTI) is commonly used to evaluate white matter integrity in multiple sclerosis (MS), but the relationship between DTI measures and functional changes during disease remains ambiguous. Using a mouse model of MS, we tested the hypothesis that DTI measures would correlate to the visual evoked potential (VEPs) dynamically at different disease stages.

Methods

In vivo DTI, gadolinium-enhanced T1WI (Gd-T1WI) and VEPs were performed in 5 control and 25 mice after 2–12 weeks of experimental autoimmune encephalomyelitis (EAE). DTI indices, including fractional anisotropy (FA), axial and radial diffusivities (AD and RD), and Gd-T1WI enhancement, were measured in the optic nerve and tract (ON and OT), which were compared with measured VEPs.

Results

Gd-T1WI showed a 3- to 4-fold enhancement over controls beginning after 2 weeks of EAE. Across the time course, we found progressive reductions in FA and increases in RD with increases in VEP latency and reductions in amplitude. Significant correlations between DTI (FA and RD) and VEP evolved; in control/early asymptomatic EAE mice, both FA and RD were highly correlated with VEP latency (but not amplitude), while in late EAE, both DTI indices were highly correlated with VEP amplitude (but not latency).

Conclusion

DTI measures FA and RD are associated to VEP latency in early stages of EAE but associated to VEP amplitude in later stages, suggesting that the patterns of DTI related to the functional decline may depend on the stage of disease progression.

Keywords

Diffusion tensor imaging (DTI) Visual evoked potential (VEP) Gd-enhanced T1WI (Gd-T1WI) Experimental autoimmune encephalomyelitis (EAE) Multiple sclerosis (MS) 

Abbreviations

DTI

Diffusion tensor imaging

VEP

Visual evoked potential

Gd-T1WI

Gd-enhanced T1-weighted imaging

EAE

Experimental autoimmune encephalomyelitis

MS

Multiple sclerosis

FA

Fractional anisotropy

MD

Mean diffusivity

AD

Axial diffusivity

RD

Radial diffusivity

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Christopher Nishioka
    • 1
    • 2
  • Hsiao-Fang Liang
    • 1
    • 3
  • Chen-Fang Chung
    • 1
  • Shu-Wei Sun
    • 1
    • 2
    • 3
  1. 1.Basic Science, School of MedicineLoma Linda UniversityLoma LindaUSA
  2. 2.Neuroscience Graduate ProgramUniversity of CaliforniaRiversideUSA
  3. 3.Pharmaceutical Science, School of PharmacyLoma Linda UniversityLoma LindaUSA

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