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The Journal of Membrane Biology

, Volume 178, Issue 1, pp 43–48 | Cite as

Characteristics of the Quenching of 9-Aminoacridine Fluorescence by Liposomes Made from Plant Lipids

  • D.K. Brauer
  • U. Yermiyahu
  • G. Rytwo
  • T.B. Kinraide
Article

Abstract

Several laboratories have determined the surface charge density of membranes utilizing methods based on vesicle-induced quenching of the fluorescence of 9-aminoacridine and its relief by other cations. However, the computational methods by which surface charge density were calculated have not been verified in a model system. In this study, the quenching of 9-aminoacridine fluorescence by liposomes made from varying amounts of digalactosyldiacylglyceride and phosphatidic acid and relief of quenching by salts was examined. Quenching of 9-aminoacridine fluorescence increased with increasing amounts of phosphatidic acid added, independent of the composition of the added liposomes. In certain instances, the computational methods did not yield the surface charge density of the liposomes expected from their composition. However, when the effects of background ionic strength on surface potential were considered, there was a positive correlation between expected and calculated values. Therefore, the data support the contention that changes in the fluorescence of 9-aminoacridine can be used to calculate surface charge density of membranes.

Key words: Surface charge density — Electrostatic attraction — Sorption — Cations — 9-Aminoacridine — Liposome — Lipid composition 

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Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • D.K. Brauer
    • 1
  • U. Yermiyahu
    • 1
  • G. Rytwo
    • 1
  • T.B. Kinraide
    • 1
  1. 1.Appalachian Farming Systems Research Center, United States Department of Agriculture, 1224 Airport Road, Post Office Box 400, Beaver, WV 25813-0400, USAUS

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