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The Journal of Membrane Biology

, Volume 251, Issue 4, pp 573–579 | Cite as

The Distributions of Voltage-Gated K+ current Subtypes in Different Cell Sizes from Adult Mouse Dorsal Root Ganglia

  • Anqi Sheng
  • Jiangru Hong
  • Lulu Zhang
  • Yan Zhang
  • Guangqin Zhang
Article

Abstract

Voltage-gated K+ (KV) currents play a crucial role in regulating pain by controlling neuronal excitability, and are divided into transient A-type currents (IA) and delayed rectifier currents (IK). The dorsal root ganglion (DRG) neurons are heterogeneous and the subtypes of KV currents display different levels in distinct cell sizes. To observe correlations of the subtypes of KV currents with DRG cell sizes, KV currents were recorded by whole-cell patch clamp in freshly isolated mouse DRG neurons. Results showed that IA occupied a high proportion in KV currents in medium- and large-diameter DRG neurons, whereas IK possessed a larger proportion of KV currents in small-diameter DRG neurons. A lower correlation was found between the proportion of IA or IK in KV currents and cell sizes. These data suggest that IA channels are mainly expressed in medium and large cells and IK channels are predominantly expressed in small cells.

Keywords

Voltage-gated K+ channel Dorsal root ganglia Cell diameter Patch clamp 

Notes

Compliance with Ethical Standards

Conflict of interest

The authors declare that they do not have conflict of interests.

References

  1. Cardenas CG, Del Mar LP, Scroggs RS (1995) Variation in serotonergic inhibition of calcium channel currents in four types of rat sensory neurons differentiated by membrane properties. J Neurophysiol 74:1870–1879.  https://doi.org/10.1152/jn.1995.74.5.1870 CrossRefPubMedGoogle Scholar
  2. Cervero F (1994) Sensory innervation of the viscera: peripheral basis of visceral pain. Physiol Rev 74:95–138.  https://doi.org/10.1152/physrev.1994.74.1.95 CrossRefPubMedGoogle Scholar
  3. Everill B, Rizzo MA, Kocsis JD (1998) Morphologically identified cutaneous afferent DRG neurons express three different potassium currents in varying proportions. J Neurophysiol 79:1814–1824CrossRefPubMedPubMedCentralGoogle Scholar
  4. Fan N, Donnelly DF, LaMotte RH (2011a) Chronic compression of mouse dorsal root ganglion alters voltage-gated sodium and potassium currents in medium-sized dorsal root ganglion neurons. J Neurophysiol 106:3067–3072.  https://doi.org/10.1152/jn.00752.2011 CrossRefPubMedPubMedCentralGoogle Scholar
  5. Fan N, Sikand P, Donnelly DF, Ma C, Lamotte RH (2011b) Increased Na+ and K+ currents in small mouse dorsal root ganglion neurons after ganglion compression. J Neurophysiol 106:211–218.  https://doi.org/10.1152/jn.00065.2011 CrossRefPubMedPubMedCentralGoogle Scholar
  6. Hayashi Y et al (2009) Bladder hyperactivity and increased excitability of bladder afferent neurons associated with reduced expression of KV1.4 alpha-subunit in rats with cystitis. Am J Physiol Regul Integr Comp Physiol 296:R1661–R1670.  https://doi.org/10.1152/ajpregu.91054.2008 CrossRefGoogle Scholar
  7. Kim DS, Choi JO, Rim HD, Cho HJ (2002) Downregulation of voltage-gated potassium channel alpha gene expression in dorsal root ganglia following chronic constriction injury of the rat sciatic nerve. Brain Res Mol Brain Res 105:146–152CrossRefPubMedGoogle Scholar
  8. MeLean MJ, Bennett PB, Thomas RM (1988) Subtypes of dorsal root ganglion neurons based on different inward currents as measured by whole-cell voltage clamp. Mol Cell Biochem 80:95–107PubMedGoogle Scholar
  9. Murakami M et al (2004) Antinociceptive effect of different types of calcium channel inhibitors and the distribution of various calcium channel alpha 1 subunits in the dorsal horn of spinal cord in mice. Brain Res 1024:122–129.  https://doi.org/10.1016/j.brainres.2004.07.066 CrossRefPubMedGoogle Scholar
  10. Petruska JC, Cooper BY, Gu JG, Rau KK, Johnson RD (2000) Distribution of P2 × 1, P2 × 2, and P2 × 3 receptor subunits in rat primary afferents: relation to population markers and specific cell types. J Chem Neuroanat 20:141–162CrossRefPubMedGoogle Scholar
  11. Petruska JC, Napaporn J, Johnson RD, Cooper BY (2002) Chemical responsiveness and histochemical phenotype of electrophysiologically classified cells of the adult rat dorsal root ganglion. Neuroscience 115:15–30CrossRefPubMedGoogle Scholar
  12. Stewart T, Beyak MJ, Vanner S (2003) Ileitis modulates potassium and sodium currents in guinea pig dorsal root ganglia sensory neurons. J Physiol 552:797–807.  https://doi.org/10.1113/jphysiol.2003.046409 CrossRefPubMedPubMedCentralGoogle Scholar
  13. Vydyanathan A, Wu ZZ, Chen SR, Pan HL (2005) A-type voltage-gated K+ currents influence firing properties of isolectin B4-positive but not isolectin B4-negative primary sensory neurons. J Neurophysiol 93:3401–3409.  https://doi.org/10.1152/jn.01267.2004 CrossRefPubMedGoogle Scholar
  14. Wang JG, Strong JA, Xie W, Zhang JM (2007) Local inflammation in rat dorsal root ganglion alters excitability and ion currents in small-diameter sensory neurons. Anesthesiology 107:322–332.  https://doi.org/10.1097/01.anes.0000270761.99469.a7 CrossRefPubMedPubMedCentralGoogle Scholar
  15. Xiao Y, Wu Y, Zhao B, Xia Z (2016) Decreased voltage-gated potassium currents in rat dorsal root ganglion neurons after chronic constriction injury. Neuroreport 27:104–109.  https://doi.org/10.1097/WNR.0000000000000505 CrossRefPubMedGoogle Scholar
  16. Xu GY, Winston JH, Shenoy M, Yin H, Pasricha PJ (2006) Enhanced excitability and suppression of A-type K+ current of pancreas-specific afferent neurons in a rat model of chronic pancreatitis. Am J Physiol Gastrointest Liver Physiol 291:G424–G431.  https://doi.org/10.1152/ajpgi.00560.2005 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical PharmacyChina Pharmaceutical UniversityNanjingChina

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