The Journal of Membrane Biology

, Volume 247, Issue 6, pp 461–468 | Cite as

Phosphoinositide 3-Kinase Pathway Mediates Early Aldosterone Action on Morphology and Epithelial Sodium Channel in Mammalian Renal Epithelia

  • Yuan Zhou
  • Xuewei Chen
  • Xiao Liu
  • Hujie Lu
  • Ying Li
  • Hui Zhu
  • Gaihong An
  • Na Zhang
  • Jianning Zhang
  • Qiang Ma
  • Yanjun Zhang


Involvement of phosphoinositide 3-kinases (PI3Ks) in early aldosterone action on epithelial sodium channel (ENaC) in mammalian renal epithelia was investigated by hopping probe ion conductance microscopy combined with patch-clamping in this study. Aldosterone treatment enlarged the cell volume and elevated the apical membrane of renal mpkCCDc14 epithelia, which resulted in enhancing the open probability of ENaC. Inhibition of PI3K pathway by LY294002 obviously suppressed these aldosterone-induced changes in both cell morphology and ENaC activity. These results indicated the important role of PI3K pathway in early aldosterone action and the close relationship between cell morphology and ENaC activity in mammalian renal epithelia.





This work was supported by the National Natural Science Foundation of China (No.30971184; 81271361; 81330029; 31300828), the International Science & Technology Cooperation Program of China (No.2011DFG33430), and Tianjin Natural Science Foundation of China (No.13JCYBJC21900; 12JCYBJC31500).

Conflict of interest

There are no conflicts of interest.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Neurosurgery, Tianjin Medical University General Hospital; Tianjin Neurological Institute; Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous SystemMinistry of Education; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous SystemTianjinChina
  2. 2.Department of Occupational HygieneInstitute of Health and Environmental MedicineTianjinChina
  3. 3.Nanomedicine LaboratoryChina National Academy of Nanotechnology & EngineeringTianjinChina
  4. 4.Medicine DivisionImperial College LondonLondonUK

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