Journal of Membrane Biology

, Volume 219, Issue 1–3, pp 71–81 | Cite as

Thyroid Hormone-Induced Alterations in Membrane Structure-Function Relationships: Studies on Kinetic Properties of Rat Kidney Microsomal Na+,K+-ATPase and Lipid/Phospholipid Profiles

  • Surendra S. Katyare
  • Hiren R. Modi
  • Samir P. Patel
  • Minal A. Patel


The effects of thyroidectomy (Tx) and subsequent treatment with 3,5,3′-triiodothyronine (T3) or combined replacement therapy (TR) with T3 and thyroxine (T4) on the substrate and temperature kinetics properties of Na+,K+-ATPase and lipid/phospholipid makeup of rat kidney microsomes were examined. Enzyme activity was somewhat high in the hypothyroid (Tx) animals and increased significantly following T3 treatment, while TR treatment caused a decrease. In the Tx and T3 groups enzyme activity resolved in two kinetic components, while in the TR group the enzyme showed allosteric behavior up to 0.5 mm ATP concentration. The K m and V max values of both the components decreased in Tx animals without affecting the catalytic efficiency. T3 treatment caused a significant increase in the V max of both the components, with a significant increase in the catalytic efficiency, while the K m values were not upregulated. The TR regimen lowered the K m and V max of component II but improved the catalytic efficiency. Thyroid status-dependent changes were also noted in the temperature kinetics of the enzyme. Regression analysis revealed that changes in the substrate and temperature kinetics parameters correlated with specific phospholipid components.


Thyroid hormone Triiodothyronine Thyroxine Microsomal Na+,K+-ATPase Substrate and temperature kinetics Lipid/phospholipid profile 


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Surendra S. Katyare
    • 1
  • Hiren R. Modi
    • 1
  • Samir P. Patel
    • 1
  • Minal A. Patel
    • 1
  1. 1.Department of Biochemistry, Faculty of ScienceThe Maharaja Sayajirao University of BarodaVadodaraIndia

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