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Journal of Membrane Biology

, Volume 219, Issue 1–3, pp 71–81 | Cite as

Thyroid Hormone-Induced Alterations in Membrane Structure-Function Relationships: Studies on Kinetic Properties of Rat Kidney Microsomal Na+,K+-ATPase and Lipid/Phospholipid Profiles

  • Surendra S. Katyare
  • Hiren R. Modi
  • Samir P. Patel
  • Minal A. Patel
Article

Abstract

The effects of thyroidectomy (Tx) and subsequent treatment with 3,5,3′-triiodothyronine (T3) or combined replacement therapy (TR) with T3 and thyroxine (T4) on the substrate and temperature kinetics properties of Na+,K+-ATPase and lipid/phospholipid makeup of rat kidney microsomes were examined. Enzyme activity was somewhat high in the hypothyroid (Tx) animals and increased significantly following T3 treatment, while TR treatment caused a decrease. In the Tx and T3 groups enzyme activity resolved in two kinetic components, while in the TR group the enzyme showed allosteric behavior up to 0.5 mm ATP concentration. The K m and V max values of both the components decreased in Tx animals without affecting the catalytic efficiency. T3 treatment caused a significant increase in the V max of both the components, with a significant increase in the catalytic efficiency, while the K m values were not upregulated. The TR regimen lowered the K m and V max of component II but improved the catalytic efficiency. Thyroid status-dependent changes were also noted in the temperature kinetics of the enzyme. Regression analysis revealed that changes in the substrate and temperature kinetics parameters correlated with specific phospholipid components.

Keywords

Thyroid hormone Triiodothyronine Thyroxine Microsomal Na+,K+-ATPase Substrate and temperature kinetics Lipid/phospholipid profile 

References

  1. Arystarkhova E, Sweadner KJ (1997) Tissue-specific expression of the Na,K-ATPase beta3 subunit. The presence of beta3 in lung and liver addresses the problem of the missing subunit. J Biol Chem 272:22405–22408PubMedCrossRefGoogle Scholar
  2. Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 234:466–468PubMedGoogle Scholar
  3. Blanco G, Mercer RW (1998) Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function. Am J Physiol 275:F633–F650PubMedGoogle Scholar
  4. Chaudhury S, Ismail-Beigi F, Gick GG, Levenson R, Edelman IS (1987) Effect of thyroid hormone on the abundance of Na,K-adenosine triphosphatase alpha-subunit messenger ribonucleic acid. Mol Endocrinol 1:83–89PubMedGoogle Scholar
  5. Dixon M, Webb EC (1979) Enzyme kinetics. In: Dixon M, Webb EC, Thorne C Jr, Tipton KF (eds), Enzymes, 3rd ed. London: Longman, pp 47–206Google Scholar
  6. Escobar-Morreale HF, del Rey FE, Obregon MJ, de Escobar GM (1996) Only the combined treatment with thyroxine and triiodothyronine ensures euthyroidism in all tissues of the thyroidectomized rat. Endocrinology 137:2490–2502PubMedCrossRefGoogle Scholar
  7. Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509PubMedGoogle Scholar
  8. Garg LC, Tisher CC (1985) Effects of thyroid hormone on Na-K-adenosine triphosphatase activity along the rat nephron. J Lab Clin Med 106:568–572PubMedGoogle Scholar
  9. Gick GG, Ismail-Beigi F, Edelman IS (1988) Thyroidal regulation of rat renal and hepatic Na,K-ATPase gene expression. J Biol Chem 263:16610–16618PubMedGoogle Scholar
  10. Hoch FL (1988) Lipids and thyroid hormones. Prog Lipid Res 27:199–270PubMedCrossRefGoogle Scholar
  11. Holmes EW Jr, Di Scala VA (1970) Studies on the exaggerated natriuretic response to a saline infusion in the hypothyroid rat. J Clin Invest 49:1224–1236PubMedGoogle Scholar
  12. Katewa SD, Katyare SS (2003a) Kinetic attributes of rat liver microsomal adenosine 5′ triphosphate phophohydrolase (ATPase). Indian J Biochem Biophys 40:252–259Google Scholar
  13. Katewa SD, Katyare SS (2003b) A simplified method for inorganic phosphate determination and its application for phosphate analysis in enzyme assays. Anal Biochem 323:180–187PubMedCrossRefGoogle Scholar
  14. Katyare SS, Rajan RR (2005) Influence of thyroid hormone treatment on respiratory activity of cerebral mitochondria from hypothyroid rats. A critical re-assessment. Exp Neurol 195:416–422PubMedCrossRefGoogle Scholar
  15. Katyare SS, Joshi MV, Fatterpaker P, Sreenivasan A (1977) Effect of thyroid deficiency on oxidative phosphorylation in rat liver, kidney and brain mitochondria. Arch Biochem Biophys 182:155–163PubMedCrossRefGoogle Scholar
  16. Katz AI, Lindheimer MD (1973) Renal sodium- and potassium-activated adenosine triphosphatase and sodium reabsorption in the hypothyroid rat. J Clin Invest 52:796–804PubMedCrossRefGoogle Scholar
  17. Katz AI, Emmanouel DS, Lindheimer MD (1975) Thyroid hormone and the kidney. Nephron 15:223–249PubMedCrossRefGoogle Scholar
  18. Kaushal R, Dave KR, Katyare SS (1999) Paracetamol hepatotoxicity and microsomal function. Environ Toxicol Pharmacol 1:67–74CrossRefGoogle Scholar
  19. Kinsella J, Sacktor B (1985) Thyroid hormones increase Na+-H+ exchange activity in renal brush border membranes. Proc Natl Acad Sci USA 82:3606–3610PubMedCrossRefGoogle Scholar
  20. Lo S-C, August TR, Liberman UA, Edelman IS (1976) Dependence of renal (Na+,K+)-adenosine triphosphatase activity on thyroid status. J Biol Chem 251:7826–7833PubMedGoogle Scholar
  21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  22. McDonough AA, Brown TA, Horowitz B, Chiu R, Schlotterbeck J, Bowen J, Schmitt CA (1988) Thyroid hormone coordinately regulates Na+-K+-ATPase alpha- and beta-subunit mRNA levels in kidney. Am J Physiol 254:C323–C329PubMedGoogle Scholar
  23. Michael UF, Barenberg RL, Chavez R, Vaamonde CA, Papper S (1972) Renal handling of sodium and water in the hypothyroid rat. Clearance and micropuncture studies. J Clin Invest 51:1405–1412PubMedGoogle Scholar
  24. Modi HR, Patel SP, Katyare SS, Patel MA (2007) Thyroid hormone treatments differentially affect kinetic properties of FoF1 ATPase and succinate oxidase and lipid/phospholipid composition of rat kidney mitochondria. A correlative study. J Membr Biol (in press)Google Scholar
  25. Pandya JD, Dave KR, Katyare SS (2004) Effect of long-term aluminum feeding on lipid/phospholipid profiles of rat brain myelin. Lipids Health Dis 3:13–18PubMedCrossRefGoogle Scholar
  26. Patel HG, Aras RV, Dave KR, Katyare SS (2000) Kinetic attributes of Na+,K+-ATPase and lipid/phospholipid profiles of rat and human erythrocyte membrane. Z Naturforsch [C] 55:770–777Google Scholar
  27. Patel SP, Katyare SS (2006a) Insulin-status-dependant alterations in lipid/phospholipid composition of rat kidney microsomes and mitochondria. Lipids 41:819–825PubMedCrossRefGoogle Scholar
  28. Patel SP, Katyare SS (2006b) Insulin-status-dependant modulation of FoF1 ATPase activity in rat kidney mitochondria. Arch Physiol Biochem 112:150–157PubMedCrossRefGoogle Scholar
  29. Robinson JD, Flashner MS (1979) The (Na+,K+)-activated ATPase. Enzymatic and transport properties. Biochim Biophys Acta 549:145–176PubMedGoogle Scholar
  30. Satav JG, Katyare SS (1991) Effect of thyroidectomy and subsequent treatment with triiodothyronine on kidney mitochondrial oxidative phosphorylation in the rat. J Biosci 16:81–89CrossRefGoogle Scholar
  31. Skipski VP, Peterson RF, Barclay M (1964) Lipid composition of human serum lipoprotein. Biochem J 90:374–378PubMedGoogle Scholar
  32. Tata JR (1964) Biological action of thyroid hormones at the cellular and molecular levels. In: Litwack G, Kritchevsky D (eds), Action of Hormones on Molecular Processes. New York: Wiley, pp 58–131Google Scholar
  33. Wetzel RK, Arystarkhova E, Sweadner KJ (1999) Cellular and subcellular specification of Na,K-ATPase alpha and beta isoforms in the postnatal development of mouse retina. J Neurosci 19:9878–9889PubMedGoogle Scholar
  34. Zlatkis A, Zak B, Boyle AJ (1953) A new method for the direct determination of serum cholesterol. J Lab Clin Med 41:486–492PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Surendra S. Katyare
    • 1
  • Hiren R. Modi
    • 1
  • Samir P. Patel
    • 1
  • Minal A. Patel
    • 1
  1. 1.Department of Biochemistry, Faculty of ScienceThe Maharaja Sayajirao University of BarodaVadodaraIndia

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