Journal of Membrane Biology

, Volume 215, Issue 2–3, pp 147–159 | Cite as

Claudin-8 Expression in Renal Epithelial Cells Augments the Paracellular Barrier by Replacing Endogenous Claudin-2

  • Susanne Angelow
  • Eveline E. Schneeberger
  • Alan S. L. YuEmail author


Claudins are transmembrane proteins of the tight junction that determine and regulate paracellular ion permeability. We previously reported that claudin-8 reduces paracellular cation permeability when expressed in low-resistance Madin-Darby canine kidney (MDCK) II cells. Here, we address how the interaction of heterologously expressed claudin-8 with endogenous claudin isoforms impacts epithelial barrier properties. In MDCK II cells, barrier improvement by claudin-8 is accompanied by a reduction of endogenous claudin-2 protein at the tight junction. Here, we show that this is not because of relocalization of claudin-2 into the cytosolic pool but primarily due to a decrease in gene expression. Claudin-8 also affects the trafficking of claudin-2, which was displaced specifically from the junctions at which claudin-8 was inserted. To test whether replacement of cation-permeable claudin-2 mediates the effect of claudin-8 on the electrophysiological phenotype of the host cell line, we expressed claudin-8 in high-resistance MDCK I cells, which lack endogenous claudin-2. Unlike in MDCK II cells, induction of claudin-8 in MDCK I cells (which did not affect levels of endogenous claudins) did not alter paracellular ion permeability. Furthermore, when endogenous claudin-2 in MDCK II cells was downregulated by epidermal growth factor to create a cell model with low transepithelial resistance and low levels of claudin-2, the permeability effects of claudin-8 were also abolished. Our findings demonstrate that claudin overexpression studies measure the combined effect of alterations in both endogenous and exogenous claudins, thus explaining the dependence of the phenotype on the host cell line.


Tight junction Claudin Paracellular transport Renal epithelia 



This work was supported by National Institutes of Health grants DK062283 (to A. S. L. Y.), HL25822 (to E. E. S.) and DK48522 (to the USC Center for Liver Diseases, for the Microscopy Subcore). We thank Joanne M. McCormack for expert technical assistance.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Susanne Angelow
    • 1
  • Eveline E. Schneeberger
    • 2
  • Alan S. L. Yu
    • 1
    Email author
  1. 1.Division of Nephrology, Department of MedicineUniversity of Southern California Keck School of MedicineLos AngelesCalifornia
  2. 2.Department of PathologyMassachusetts General HospitalBostonMassachusetts

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