The Journal of Membrane Biology

, Volume 199, Issue 2, pp 113–118 | Cite as

Optimal-Sensitivity Analysis of Ion Channel Gating Kinetics

Article

Abstract

We propose a new approach to analysis of kinetic models for ion channel gating, based on application of fluctuating voltages through a voltage clamp, in addition to conventional techniques. We show that the channel kinetics can be probed in a much more sensitive way, leading to more efficient model selection and more reliable estimates of model parameters. We use wavelet transform as an analytic tool for fluctuating currents and parametric dispersion plots as a measure of model compatibility with experimental data.

Keywords

Voltage-gated ion channels Markov models Nonequilibrium response spectroscopy Wavelets 

References

  1. Bezanilla, F., Peroso, E., Stefani, E. 1994Gating of Shaker K+ channels II: the components of gating currents and a model of channel activationBiophys. J.6610111021PubMedGoogle Scholar
  2. Daubechies, I. 1992Ten Lectures on WaveletsSIAMPhiladelphia, PAGoogle Scholar
  3. Hille, B. 2001Ionic Channels of Excitable Membranes. 3rd edSinauer Associates Inc.Sunderland, MAGoogle Scholar
  4. Hodgkin, A.L., Huxley, A.F. 1952A quantitative description of membrane current and its application to conduction and excitation in nerveJ. Physiol.117500544PubMedGoogle Scholar
  5. Hosein-Sooklal, A., Kargol, A. 2002Wavelet Analysis of Nonequilibrium Ionic Currents in Human Heart Sodium Channel (hH1a)J. Membrane Biol.188199212CrossRefGoogle Scholar
  6. Kargol, A., Smith, B., Millonas, M.M. 2002Applications of nonequilibrium response spectroscopy to the study of channel gating. Experimental design and optimizationJ. Theoret. Biol.218239258CrossRefGoogle Scholar
  7. Millonas, M.M., Hanck, D.A. 1998aNonequilibrium response spectroscopy and the molecular kinetics of proteinsPhys. Rev. Lett.80401404CrossRefGoogle Scholar
  8. Millonas, M.M., Hanck, D.A. 1998bNonequilibrium response spectroscopy of voltage-sensitive ion channel gatingBiophys. J.74210229Google Scholar
  9. Sakmann, B., Neher, E. 1995Single Channel Recording Plenum, New YorkBiophys. J.74210229Google Scholar
  10. Schoppa, N.E., Sigworth, F.J. 1998Activation of Shaker potassium channels I. Characterization of voltage-dependent transitionsJ. Gen. Physiol.111271294CrossRefPubMedGoogle Scholar
  11. Vandenberg, C.A., Bezanilla, F. 1991A sodium channel gating model based on single channel, macroscopic ionic and gating currents in the squid giant axonBiophys. J.6015111533PubMedGoogle Scholar
  12. Zagotta, W.N., Hoshi, T., Aldrich, R.W. 1994Shaker potassium channel gating III: evaluation of kinetic models for activationJ. Gen. Physiol.103321362CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Physics DepartmentLoyola University New OrleansNew OrleansUSA
  2. 2.Department of Physiology and BiophysicsUniversity of Arkansas for Medical SciencesLittle RockUSA

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