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The Journal of Membrane Biology

, Volume 195, Issue 1, pp 21–26 | Cite as

Kinetics of Influenza Virus Fusion with the Endosomal and Plasma Membranes of Cultured Cells. Effect of Temperature

  • I. Nunes-Correia
  • S. Nir
  • M. C. Pedroso de Lima
Article

Abstract

We performed a detailed kinetic analysis of influenza virus fusion with the endosomal and plasma membranes of Madin Darby canine kidney (MDCK) cells and provided a comparison of the kinetic parameters obtained for both cases at 20°C and 37°C. Using our mass action kinetic model, we determined that the fusion rate constant, f, for influenza virus with the endosomal membrane was 0.02 s−1 at 37°C and 0.0035 s−1 at 20°C. The analysis of the fusion kinetics of influenza virus with the plasma membrane yielded that the fusion rate constants were close to those deduced with the endosomal membrane. The systematic kinetic analysis performed in this study provides for the first time a biophysical support for studies on influenza virus-cell fusion where the acidic endosomal internal environment is simulated artificially by lowering the pH of the medium.

Keywords

Influenza virus Endocytosis Membrane fusion MDCK cells Kinetic analysis 

Notes

Acknowledgements

This work was supported by Grant POCTI/CVT/42700/2001 from FCT, Portugal (I. N.-C.), by European Union Grant BIO4-CT97-2191 (M. C. P. L.), and by Grant PRAXIS/PCNA/P/BIO/45/96 from JNICT, Portugal (M. C. P. L.).

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Copyright information

© Springer-Verlag New York Inc. 2003

Authors and Affiliations

  • I. Nunes-Correia
    • 1
    • 2
  • S. Nir
    • 3
  • M. C. Pedroso de Lima
    • 1
    • 2
  1. 1.Department of BiochemistryUniversity of Coimbra, Apartado 3126, 3000 CoimbraPortugal
  2. 2.Center for Neuroscience and Cell BiologyUniversity of Coimbra, 3000 CoimbraPortugal
  3. 3.Seagram Center for Soil and Water Sciences, Faculty of Agricultural, Food and Environmental Quality SciencesHebrew University of Jerusalem, Rehovot 76100Israel

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