Liquid temperature measurement method in microchannels by using fluorescence polarization

  • Kazuya Tatsumi
  • Chi Hsuan Hsu
  • Atsushi Suzuki
  • Kazuyoshi Nakabe
Original
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Abstract

A novel optical method that can measure fluid temperature at the microscopic scale by measuring fluorescence polarization is described in this paper. The measurement is much less influenced by fluorescence quenching effects, which is a significant issue in conventional laser-induced fluorescence methods. Therefore, the effects of the other properties of the fluid can be reduced considerably in the proposed method, thus has the potential of leading to greater reliability in measuring the temperature. An experiment was performed in a microchannel flow by using fluorescent molecule probes. The relationship between the fluid temperature and the measured fluorescence polarization degree is evaluated to derive the correlation curve. In addition, the effects of the fluid viscosity and fluid pH on the fluorescence polarization degree are discussed to evaluate the influence of the quenching effects. The results showed that the fluorescence polarization is considerably less sensitive to the quenching factors as compared with the fluorescence intensity measurements. Furthermore, a strong linear correlation between the polarization degree and the fluid temperature was obtained. This relationship agreed well with the theoretical one qualitatively and confirmed the validity of the measurements and feasibility of the proposed method.

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Notes

Acknowledgments

This work was partially supported by the Japan Society for the Promotion of Science KAKENHI Grant Number 15H03931, SPIRITS (Supporting Program for Interaction-based Initiative Team Studies), and Micro/Nano Fabrication Hub in Kyoto University of “Low-Carbon Research Network” funded by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Department of Mechanical Engineering and ScienceKyoto UniversityKyotoJapan

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