Marine Biology

, Volume 137, Issue 2, pp 239–244

Localization of RubisCO and sulfur in endosymbiotic bacteria of the gutless marine oligochaete Inanidrilus leukodermatus (Annelida)

  • J. Krieger
  • O. Giere
  • N. Dubilier

Abstract

 The chemoautotrophic potential of the two co-occurring larger and smaller bacterial endosymbionts of the gutless marine oligochaete Inanidrilus leukodermatus was determined using immunocytochemistry. An antibody directed against the Form I of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), the key CO2-fixing enzyme of the Calvin–Benson cycle, consistently labeled the larger symbionts. Electron microscopic spectroscopy showed that the larger symbionts contained sulfur in intracellular globules and to a lesser degree in the cytoplasm. The presence of RubisCO and sulfur indicates that the larger endosymbionts of I. leukodermatus are chemoautotrophic sulfur-oxidizing bacteria. In contrast, no RubisCO or sulfur was detected in the smaller endosymbionts of this host.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • J. Krieger
    • 1
  • O. Giere
    • 1
  • N. Dubilier
    • 2
  1. 1.Zoological Institute and Zoological Museum, University of Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany Tel.: +49-40-428384234; Fax: +49-40-428383937 e-mail: jens.krieger@zoologie.uni-hamburg.deDE
  2. 2.Max-Planck-Institute for Marine Microbiology, Celsiusstraße 1, 28359 Bremen, GermanyDE

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