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Marine Biology

, 165:50 | Cite as

Morphological and epigenetic variation in mussels from contrasting environments

  • R. G. A. Watson
  • Simone Baldanzi
  • A. Pérez-Figueroa
  • G. Gouws
  • F. Porri
Original paper

Abstract

The impact of contrasting environments on organisms can result in the establishment of distinct phenotypic traits. Environmentally induced epigenetic mechanisms directly regulate gene expression and potentially lead to long-lasting effects. How phenotypic, epigenetic, and genetic components of wild populations relate to each other is, however, still largely debated. We examined the effect of broad coastline topography (as bay versus open coast) on the morphological, genetic, and epigenetic (i.e., DNA methylation) traits of the brown mussel Perna perna from four natural populations along the south coast of South Africa (between 33.9 S, 25.7 E and 34.2 S, 22.1 E) collected in April 2014. Several morphometric measurements were taken on the mussel body and byssal thread. The epigenetic and genetic structure of P. perna was assessed using the methylation sensitive analysis of polymorphisms technique. Morphological traits differed among populations, but no clear effect of topography on both morphology and genetics was found. Bay and Open Coast sites differed in the patterns of DNA methylation of selected loci, suggesting that topography shaped the epigenetic profile of populations of P. perna. The environmentally induced changes in the DNA methylation of selected loci were neither correlated with the morphological traits analysed, nor explained by the underlying genetic variance among populations. The relationship amongst epigenetics, morphology, and genetics of P. perna populations was shown to be complex and dynamic. Although inconsistent, the topographically linked variability in epigenetic and the phenotypic differences in genetically close populations of mussels highlights the potential role of the local environment in driving mesoscale differences among populations.

Notes

Acknowledgements

We thank N. Weidberg, D. Sousoni, and J. Bueno for assistance during the field collection and T. Bodill for assistance in the molecular laboratory. FP wishes to thank Dr Dittmar Eichoff for early discussions on epigenetics and sharing ideas and applications on orthodontics and marine ecology. We finally thank two anonymous reviewers for their valuable contribution on the revision of this paper.

Funding

This contribution is based upon research supported by funds provided by the South African Institute for Aquatic Biodiversity.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed.

Supplementary material

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Supplementary material 1 (PDF 247 kb)
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Supplementary material 2 (HTML 1056 kb)

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Authors and Affiliations

  1. 1.South African Institute for Aquatic Biodiversity (SAIAB)GrahamstownSouth Africa
  2. 2.Nucleo Milenio, Centro de Conservación Marina and Estación Costera de Investigaciones MarinasPontificia Universidad Católica de ChileSantiagoChile
  3. 3.Departamento de Bioquímica, Genética e InmunologíaUniversidad de VigoVigoSpain
  4. 4.The Department of Zoology and EntomologyRhodes UniversityGrahamstownSouth Africa

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