Effects of sample treatment on the analysis of stable isotopes of carbon and nitrogen in zooplankton, micronekton and a filter-feeding shark
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Stable isotope analysis is often used to investigate the trophic ecology of marine systems. However, a lack of standardization of the treatment of samples prior to analysis, hampers comparisons of results within and among studies. This study examined the effects of lipid extraction (LE), acidification for the removal of inorganic carbonate (RIC) and rinsing with deionized water (DIW) on δ13C and δ15N values and C:N ratios in sub-dermal tissue of whale sharks (Rhincodon typus), zooplankton (>200–1000 μm) and a wide range of micronekton (>1000 μm) taxa collected in 2013 and 2014 at Ningaloo Reef (Western Australia). For whale shark tissue, lipid extraction (LE and LE + DIW) increased values of δ13C, whereas LE, LE + DIW and DIW treatments increased values of δ5N and C:N ratios. These results confirm the need to remove lipids and 15N-depleted nitrogenous waste from elasmobranch tissues. The LE + DIW treatment was the most efficient at achieving this goal. For zooplankton and micronekton, LE and RIC treatments had consistent effects on δ13C values, however, effects on δ15N values were more unpredictable. Therefore, zooplankton and micronekton samples should be split into two portions, one subjected to LE or LE + RIC treatments to standardize δ13C values, and a second untreated portion used for analysis of δ15N values. For these taxa, the RIC + DIW treatment resulted in the greatest change in δ15N values, which may confound results. Mathematical normalization models used to predict outcomes of treatments on values of δ13C and δ15N were not found to be suitable for all the taxa in this study.
- Ihaka R, Gentleman R (1996) A language for data analysis and graphics. J Comput Graph Stat 5:299–314Google Scholar
- Ivlev A, Knyazev YA, Logachev M (1996) Daily average carbon isotope composition of CO2 of expired air and urine in norm and some endocrine pathologies in man. Biofizika 41(2):508–516Google Scholar
- King P, Kennedy H, Newton PP, Jickells TD, Brand T, Calvert S, Cauwet G, Etcheber H, Head B, Khripounoff A, Manighetti B, Miquel JC (1998) Analysis of total and organic carbon and total nitrogen in settling oceanic particles and a marine sediment: an inter-laboratory comparison. Mar Chem 60:203–216CrossRefGoogle Scholar
- Olson KR (1999) Rectal gland and volume homeostasis. In: Hamlett WC (Eds.) Sharks, skates and rays: the biology of elasmobranchs fishes. The John Hopkins University Press Baltimore, Maryland, pp 329–352Google Scholar
- Yurkowski DJ, Ferguson S, Choy ES, Loseto LL, Brown TM, Muir DCG, Semeniuk CAD, Fisk AT (2016) Latitudinal variation in ecological opportunity and intraspecific competition indicates differences in niche variability and diet specialization of Arctic marine predators. Ecol Evol 6(6):1666–1678CrossRefGoogle Scholar