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Marine Biology

, Volume 162, Issue 12, pp 2513–2519 | Cite as

Viability, growth and domoic acid toxicity of the diatom Nitzschia bizertensis following filtration by the mussel Mytilus sp.

  • Donia Bouchouicha-Smida
  • Stephen S. Bates
  • Nina Lundholm
  • Christophe Lambert
  • Hassine Hadj Mabrouk
  • Asma Sakka Hlaili
Invasive Species - Original Paper
Part of the following topical collections:
  1. Invasive Species

Abstract

The diatom Nitzschia bizertensis was recently discovered in Bizerte Lagoon (Tunisia, SW Mediterranean Sea), where it was shown to produce the amnesic shellfish poisoning toxin, domoic acid (DA). This diatom was previously found to be widespread in both time and space throughout the lagoon. The present study was carried out to test the hypothesis that N. bizertensis is able to survive after being filtered and ejected as biodeposits (faeces plus pseudofaeces) by mussels. N. bizertensis cultures (strains BD4 and BD8), at natural bloom concentrations, were fed to mussels (Mytilus sp.). The stomach contents (after 1 h) and biodeposits (after 48 h) were observed for the presence and viability of N. bizertensis cells. Light microscopy revealed intact and partial cells of N. bizertensis in both the stomach contents and biodeposits of the mussels. Incubation of the biodeposits resulted in the regrowth of N. bizertensis. This provides evidence that transplanted mussels have the potential to serve as vectors for the transport of N. bizertensis. Furthermore, cells regrown from the biodeposits contained DA, but at levels lower than those in pre-filtered cells (up to 11.9 fg cell−1). Thus, regrown cells remain toxigenic. The results suggest that one must guard against spreading N. bizertensis and other DA producers via the transfer of shellfish from contaminated to pristine areas.

Keywords

Dinoflagellate Domoic Acid Harmful Alga Bizerte Lagoon Inverted Epifluorescence Microscope 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank Hansy Haberkorn, Hélène Hégaret, Marcel Koken and Nelly Le Goïc for their technical assistance with carrying out the experiments.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Donia Bouchouicha-Smida
    • 1
  • Stephen S. Bates
    • 2
  • Nina Lundholm
    • 3
  • Christophe Lambert
    • 4
  • Hassine Hadj Mabrouk
    • 1
  • Asma Sakka Hlaili
    • 1
  1. 1.Laboratoire de Cytologie Végétale et Phytoplanctonologie, Département des Sciences de la Vie, Faculté des Sciences de BizerteUniversité de CarthageZarzouna, BizerteTunisia
  2. 2.Fisheries and Oceans CanadaGulf Fisheries CentreMonctonCanada
  3. 3.Natural History Museum of DenmarkUniversity of CopenhagenCopenhagen KDenmark
  4. 4.Laboratoire des Sciences de l’Environnement Marin, UMR CNRS 6539Institut Universitaire Européen de la MerPlouzanéFrance

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