Marine Biology

, Volume 161, Issue 3, pp 551–563

Determining the diet of larvae of the red rock lobster (Jasus edwardsii) using high-throughput DNA sequencing techniques

  • R. O’Rorke
  • S. D. Lavery
  • M. Wang
  • S. D. Nodder
  • A. G. Jeffs
Original Paper

DOI: 10.1007/s00227-013-2357-7

Cite this article as:
O’Rorke, R., Lavery, S.D., Wang, M. et al. Mar Biol (2014) 161: 551. doi:10.1007/s00227-013-2357-7

Abstract

The diet of Jasus edwardsii during its ~2-year larval (phyllosoma) phase is largely unknown. High mortalities experienced during larviculture might be reduced if their diet were nutritionally modelled on the natural diet. Here, prey species were identified from phyllosoma midgut glands using 454 pyrosequencing of 18S rDNA. We found that gelatinous zooplankton, particularly Siphonophora and Ctenophora, occurred frequently in the midgut glands of phyllosomas, resolving previous conjecture that these animals are in the diet of J. edwardsii phyllosomas. A high occurrence of sequencing reads from unicellular microbes may also reflect a reliance on scavenging detritus.

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • R. O’Rorke
    • 1
  • S. D. Lavery
    • 1
    • 2
  • M. Wang
    • 2
  • S. D. Nodder
    • 3
  • A. G. Jeffs
    • 2
  1. 1.School of Biological SciencesUniversity of AucklandAucklandNew Zealand
  2. 2.Institute of Marine ScienceUniversity of AucklandWarkworthNew Zealand
  3. 3.National Institute of Water and Atmospheric Research (NIWA) Ltd.Kilbirnie, WellingtonNew Zealand

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