Marine Biology

, Volume 160, Issue 6, pp 1395–1406 | Cite as

New insight on population genetic connectivity of widespread amphidromous prawn Macrobrachium lar (Fabricius, 1798) (Crustacea: Decapoda: Palaemonidae)

  • Magalie Castelin
  • Pierre Feutry
  • Mélyne Hautecoeur
  • Gérard Marquet
  • Daisy Wowor
  • Gabrielle Zimmermann
  • Philippe Keith
Original Paper


Due to the sparse and unstable nature of insular freshwater habitats, marine larval dispersal of amphidromous species is considered a critical element of population persistence. We assessed population genetic structure of freshwater prawn Macrobrachium lar across its range that encompasses two biogeographic barriers: the vast open ocean separating Western and Central Pacific regions and the Indo-Malay archipelago separating Indian and Pacific oceans. A total of 173 samples collected from 21 islands throughout the Indo-Pacific were sequenced at 16S and 28S rDNA. We observed distinct genetic isolation of populations located at the eastern and southwestern edge of the species range but no evidence of an effect of the Indo-Pacific barrier. Differentiation patterns are consistent with a stepping-stone model of dispersal. Genetic differences of Central Pacific populations may reflect founder events associated with colonization of isolated islands, or be a signature of a past bottleneck after population depletion caused by drastic climatic events.


Reef Fish Population Genetic Structure Haplotype Network Larval Dispersal Pelagic Larval Duration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank all the partners that have financially supported this work: (1) the New Caledonian Government; (2) the Muséum national d’Histoire naturelle; (3) the Indonesian Institute of Sciences; (4) the ATM Barcode (PIs: Sarah Samadi and Jean-Noël Labat); and (5) the “Service de Systématique Moléculaire” (UMS 2700 CNRS-MNHN). This project was also supported by the network “Bibliothèque du Vivant” funded by the CNRS, the Muséum national d’Histoire naturelle, the INRA, and the CEA (Centre National de Séquençage). We thank the Vanuatu Environment Unit (D. Kalfatak) and the New Caledonian North and South Provinces (J-J. Cassan and C. Méresse) for allowing sampling (permit No 1224-08/PS), and ARDA (Association Réunionaise de Développement de l’Aquaculture) in Réunion Island for giving us samples. This study was made possible through assistance in sampling expeditions and field contact: P. Bosc, P. Gaucher, P. Gerbeaux, H. Grondin, R.K. Hadiaty, D. Kalfatak, G. MacCormack, N. Mary, J.Y. Meyer, Mulyadi, A. Mun’im, T. Robinet, S. Sauri, G. Ségura, L. Taillebois, P. Valade, and Wahyudin. We would like to thank C. Lord, B. Lynch, and C. Abbott for constructive comments and English improvement of the manuscript. We are grateful to the referees and to Timothy J. Page for their productive review of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

All research presented in the manuscript was conducted in accordance with all applicable laws and rules set forth by their governments and institutions, and all necessary permits were in hand when the research was conducted.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Magalie Castelin
    • 1
    • 2
  • Pierre Feutry
    • 1
    • 3
  • Mélyne Hautecoeur
    • 1
  • Gérard Marquet
    • 1
  • Daisy Wowor
    • 4
  • Gabrielle Zimmermann
    • 1
  • Philippe Keith
    • 1
  1. 1.Biologie des Organismes et Ecosystèmes Aquatiques, UMR 7208, Département Milieux et Peuplements AquatiquesMuséum national d’Histoire naturelleParis Cedex 05France
  2. 2.Fisheries and Oceans CanadaNanaimoCanada
  3. 3.Research Institute for Environment and LivelihoodsCharles Darwin UniversityDarwinAustralia
  4. 4.Division of Zoology, Research Center for BiologyIndonesian Institute of Sciences (LIPI)CibinongIndonesia

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