A molecular method for the identification of resting eggs of acartiid copepods in the Thau lagoon, France
Acartia and Paracartia species, often known to co-occur, can exhibit complex life cycles, including the production of resting eggs. Studying and understanding their population dynamics is hindered by the inability to identify eggs and early developmental stages using morphological techniques. We have developed a simple molecular technique to distinguish between the three species of the Acartiidae family (Acartia clausi, A. discaudata and Paracartia grani) that co-occur in the Thau lagoon (43°25′N; 03°40′E) in southern France. Direct amplification of a partial region of the mitochondrial cytochrome oxidase I gene by polymerase chain reaction and subsequent restriction fragment length polymorphism results in a unique restriction profile for each species. The technique is capable of determining the identity of individual eggs, including resting eggs retrieved from sediment samples, illustrating its application in facilitating population dynamic studies of this ubiquitous and important member of the zooplankton community.
- Boyer S, Arzul I, Bonnet D (2012) Some like it hot: Paracartia grani (Copepoda: Calanoida) arrival in the Thau lagoon (South of France- Mediterranean Sea). Marine biodiversity records 5: available at http://dx.doi.org/10.1017/S1755267212000565
- Boyer S, Bouvy M, Bonnet D (submitted) What triggers Acartia species egg production in a Mediterranean lagoon? Estuar Coast Shelf SciGoogle Scholar
- Fatemi M (1938) Les variations saisonnières du plancton de l’Etang de Thau à l’embouchure du canal de Sète. PhD Thesis Université Montpellier 2, France, p 97Google Scholar
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotech 3(5):294–299Google Scholar
- Onbé T (1978) Sugar flotation method for sorting the resting eggs of marine cladocerans and copepods from sea-bottom sediment. Bull Jpn Soc Sci Fish 44(12):1411Google Scholar
- Tritle D (2006) Activity of Promega Restriction Enzymes in GoTaq® Green and PCR Master Mixes. Promega Corporation Web site. http://www.promega.com/resources/articles/pubhub/enotes/activity-of-promega-restriction-enzymes-in-gotaq-green-and-pcr-master-mixes/ Updated 2006. Accessed 11th Jan 2012