Identifying trophic variation in a marine suspension feeder: DNA- and stable isotope-based dietary analysis in Mytilus spp.
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Accurate field data on trophic interactions for suspension feeders are lacking, and new approaches to dietary analysis are necessary. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was integrated with stable isotope analysis to examine dietary patterns in suspension-feeding Mytilus spp. from seven spatially discrete locations within a semi-enclosed marine bay (Strangford Lough, Northern Ireland) during June 2009. Results of the two methods were highly correlated, reflecting dietary variation in a similar manner. Variation in PCR-DGGE data was more strongly correlated with the principal environmental gradient (distance from the opening to the Irish Sea), while values of δ13C and δ15N became progressively enriched, suggesting a greater dependence on animal tissue and benthic microalgae. Diatoms and crustaceans were the most frequently observed phylotypes identified by sequencing, but specific DNA results provided little support for the trophic trends observed in the stable isotope data. This combined approach offers an increased level of trophic insight for suspension feeders and could be applied to other organisms.
- Anderson MJ, Gorley RN, Clarke KR (2008) PERMANOVA + for PRIMER: Guide to software and statistical methods. PRIMER-E, PlymouthGoogle Scholar
- Clarke KR, Gorley RN (2006) PRIMER v6: user manual/tutorial. PRIMER-E, PlymouthGoogle Scholar
- Decottignies P, Beninger PG, Rince Y, Riera P (2007) Trophic interactions between two introduced suspension-feeders, Crepidula fornicata and Crassostrea gigas, are influenced by seasonal effects and qualitative selection capacity. J Exp Mar Biol Ecol 342:231–241. doi:10.1016/j.jembe.2006.10.005 CrossRefGoogle Scholar
- Drummond AJ, Ashton B, Cheung M, Heled J, Kearse M, Moir R, Stones-Havas S, Thiere T, Wilson A (2009) Geneious v5.0.4, Available from http://www.geneious.com/
- Erwin DG (1986) Strangford Lough benthos and the marine community concept. PhD dissertation, Queen’s University BelfastGoogle Scholar
- Fukumori K, Oi M, Doi H, Takahashi D, Okuda N, Miller TW, Kuwae M, Miyasaka H, Genkai-Kato M, Koizumi Y, Omori K, Takeoka H (2008) Bivalve tissue as a carbon and nitrogen isotope baseline indicator in coastal ecosystems. Estuar Coast Shelf Sci 79:45–50. doi:10.1016/j.ecss.2008.03.004 CrossRefGoogle Scholar
- Galtsoff PS (1964) The American Oyster Crassostrea virginica Gmelin. Fish Bull US Fish Wildlife Ser 64:1–456Google Scholar
- Leal JCM, Dubois S, Orvain F, Galois R, Blin JL, Ropert M, Bataille MP, Ourry A, Lefebvre S (2008) Stable isotopes (delta C-13, delta N-15) and modelling as tools to estimate the trophic ecology of cultivated oysters in two contrasting environments. Mar Biol 153:673–688. doi:10.1007/s00227-007-0841-7 CrossRefGoogle Scholar
- Lefebvre S, Leal JCM, Dubois S, Orvain F, Blin JL, Bataille MP, Ourry A, Galois R (2009) Seasonal dynamics of trophic relationships among co-occurring suspension-feeders in two shellfish culture dominated ecosystems. Estuar Coast Shelf Sci 82:415–425. doi:10.1016/j.ecss.2009.02.002 CrossRefGoogle Scholar
- Lotsy JP (1895) The food of the oyster, clam, and ribbed mussel. Rept US Comm Fish 1893(19):375Google Scholar