Marine Biology

, Volume 160, Issue 2, pp 479–490 | Cite as

Identifying trophic variation in a marine suspension feeder: DNA- and stable isotope-based dietary analysis in Mytilus spp.

  • Aaron P. Maloy
  • Peter Nelle
  • Sarah C. Culloty
  • John W. Slater
  • Chris Harrod
Original Paper

Abstract

Accurate field data on trophic interactions for suspension feeders are lacking, and new approaches to dietary analysis are necessary. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was integrated with stable isotope analysis to examine dietary patterns in suspension-feeding Mytilus spp. from seven spatially discrete locations within a semi-enclosed marine bay (Strangford Lough, Northern Ireland) during June 2009. Results of the two methods were highly correlated, reflecting dietary variation in a similar manner. Variation in PCR-DGGE data was more strongly correlated with the principal environmental gradient (distance from the opening to the Irish Sea), while values of δ13C and δ15N became progressively enriched, suggesting a greater dependence on animal tissue and benthic microalgae. Diatoms and crustaceans were the most frequently observed phylotypes identified by sequencing, but specific DNA results provided little support for the trophic trends observed in the stable isotope data. This combined approach offers an increased level of trophic insight for suspension feeders and could be applied to other organisms.

Supplementary material

227_2012_2105_MOESM1_ESM.docx (101 kb)
Supplementary material 1 (DOCX 101 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Aaron P. Maloy
    • 1
    • 2
  • Peter Nelle
    • 3
    • 4
  • Sarah C. Culloty
    • 1
  • John W. Slater
    • 2
  • Chris Harrod
    • 3
    • 5
  1. 1.Aquaculture and Fisheries Development Centre, School of Biological, Earth and Environmental SciencesUniversity College CorkCorkIreland
  2. 2.Centre of Applied Marine Biotechnology (CAMBio)Letterkenny Institute of TechnologyLetterkennyIreland
  3. 3.School of Biological SciencesQueen’s University of BelfastBelfastUK
  4. 4.Zentrum für Didaktik der Biologie, WestfälischeWilhelms-Universität MünsterMünsterGermany
  5. 5.Instituto de Investigaciones OceanológicasUniversidad de AntofagastaAntofagastaChile

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