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Marine Biology

, Volume 158, Issue 9, pp 2027–2041 | Cite as

Mitochondrial DNA sequence variation in spiny lobsters: population expansion, panmixia, and divergence

  • Eugenia Naro-MacielEmail author
  • Brendan Reid
  • Katherine E. Holmes
  • Daniel R. Brumbaugh
  • Meredith Martin
  • Rob DeSalle
Original Paper

Abstract

To investigate population differentiation in a comparative and historical context, segments of the mitochondrial cytochrome c oxidase subunit I gene and the control region were sequenced in Panulirus argus from nine sites along approximately 1,500 km of the Northern Caribbean Sea (n = 326) and analyzed with respect to available panulirid data. A mismatch analysis and Fu’s FS test uncovered a signature of historical population expansion around the time of the Last Glacial Maximum. Significant population structure was not detected in the area. The data supported a hypothesis of panmixia resulting from ongoing larval transport by ocean currents and historical population expansion. Despite high intraspecific divergence levels at COI within Panulirus argus and several other Panulirus species, genetic species identification through DNA barcoding was feasible using either a modified distance threshold or a character-based approach.

Keywords

Last Glacial Maximum Population Expansion Mismatch Distribution Spiny Lobster Pairwise Divergence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

For samples or unpublished data, we thank Kenny Broad, Kevin Buch, Seinen Chow, Alondra Diaz, Nariaki Inoue, Aurea Rodriguez, Shane K. Sarver, Hideo Sekiguchi, Jeffrey Silberman, Patrick J. Walsh, and K and B EZ Dive in Bimini. Craig Dahlgren, Elizabeth Hemond, Carrie Kappel, and other members of the Bahamas Biocomplexity Project provided additional field assistance. We thank the students funded through NSF’s Research Experience for Undergraduates program, Ballington Kinloch, Catherine Munsch, Anthony Petroso, Caroline Storer, and Matthew Winfield, for laboratory assistance, and Steve Palumbi and Tom Oliver for discussions about this research. We are grateful to Jeffrey Silverman for producing Fig. 1. We thank The Bahamas Department of Marine Resources, the Department of Environment and Coastal Resources in the Turks and Caicos Islands, and the Departamento de Recursos Naturales y Ambientales of Puerto Rico for providing research permits for our work, as well as the School for Field Studies in the Turks and Caicos. E.N.M., K.E.H., and D.R.B. were supported in part by an NSF Biocomplexity in the Environment grant (OCE-0119976) to D.R.B. Additional support was provided by NOAA Grant #NA05SEC4691002, as well as George Amato and the AMNH’s Center for Conservation Genetics, and Eleanor Sterling and the Center for Biodiversity and Conservation.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Eugenia Naro-Maciel
    • 1
    • 2
    Email author
  • Brendan Reid
    • 3
  • Katherine E. Holmes
    • 4
    • 5
  • Daniel R. Brumbaugh
    • 4
  • Meredith Martin
    • 2
  • Rob DeSalle
    • 2
  1. 1.Biology DepartmentCollege of Staten Island, City University of New YorkStaten IslandUSA
  2. 2.Sackler Institute for Comparative GenomicsAmerican Museum of Natural HistoryNew YorkUSA
  3. 3.Department of Forest and Wildlife EcologyUniversity of WisconsinMadisonUSA
  4. 4.Center for Biodiversity and ConservationAmerican Museum of Natural HistoryNew YorkUSA
  5. 5.Wildlife Conservation SocietyPNG Marine ProgramKaviengPapua New Guinea

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