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Marine Biology

, Volume 158, Issue 5, pp 1043–1055 | Cite as

Stable isotope analysis in two sympatric populations of bottlenose dolphins Tursiops truncatus: evidence of resource partitioning?

  • Ruth Fernández
  • Susana García-Tiscar
  • M. Begoña Santos
  • Alfredo López
  • Jose A. Martínez-Cedeira
  • Jason Newton
  • Graham J. Pierce
Original Paper

Abstract

Skin and muscle from 43 bottlenose dolphins (38 juveniles/adults, 5 calves) stranded in NW Spain were analysed to determine whether stable isotope ratios (δ13C and δ15N) could be used to assess dietary variation, habitat segregation and population substructure. Results were compared with published stomach contents data. Stable isotope ratios from 17 known prey species were also determined. Isotope ratios of the main prey (blue whiting, hake) varied significantly in relation to fish body size. Dolphin calves showed significant heavy isotope enrichments compared to adult females. Excluding calves, δ15N decreased with increasing dolphin body size, probably related to an ontogenetic shift in diet towards species at lower trophic levels, e.g. on blue whiting as suggested by stomach content results. Bottlenose dolphins were divided into two putative populations (North, South) based on previous genetic studies, and values of δ13C and δ15N differed significantly between these two groups, confirming the existence of population structuring.

Keywords

Trophic Level Enrichment Factor Stable Isotope Ratio Bottlenose Dolphin Lower Trophic Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Ester Molina at the Universidad Autónoma de Madrid and Rona McGill at SUERC (Scottish Universities Environmental Research Centre) for their help with sample preparation. The authors gratefully acknowledge the assistance of volunteers from the Galician strandings network (CEMMA) and the collaboration of Jorge Millos, Jesús Estévez and Antonio Palanca from the University of Vigo. We also thank Antonio González and Lorena Rodríguez from the IEO (Instituto Español de Oceanografía), María del Carmen García, José Luís Fernández and Alfonso Fernández for providing fish and cephalopod samples. Thanks to Colin MacLeod and Stuart Piertney at the University of Aberdeen for useful comments during data analysis and discussion. R.F. was supported during the research period through a Postgraduate grant by Fundación La Caixa and a Marie Curie Early-Stage Research Grant (ECOSUMMER project. 020501-2). The stable isotope analyses were carried out in the NERC (Natural Environment Research Council) Life Sciences Mass Spectrometry Facility (Award number. EK115-10/07). G.J.P. was supported by the ANIMATE project (MEXC-CT-2006-042337).

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ruth Fernández
    • 1
    • 2
  • Susana García-Tiscar
    • 3
  • M. Begoña Santos
    • 4
  • Alfredo López
    • 2
  • Jose A. Martínez-Cedeira
    • 2
  • Jason Newton
    • 5
  • Graham J. Pierce
    • 1
    • 4
  1. 1.School of Biological SciencesUniversity of AberdeenAberdeenScotland, UK
  2. 2.CEMMAGondomarSpain
  3. 3.Ecology DepartmentUniversidad Autónoma de MadridMadridSpain
  4. 4.Instituto Español de OceanografíaCanido, VigoSpain
  5. 5.NERC Life Sciences Mass Spectrometry FacilitySUERCEast KilbrideScotland, UK

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