Marine Biology

, 156:2641

DNA barcoding of Pacific Canada’s fishes

  • Dirk Steinke
  • Tyler S. Zemlak
  • James A. Boutillier
  • Paul D. N. Hebert
Comment and Reply

Abstract

DNA barcoding—sequencing a standard region of the mitochondrial cytochrome c oxidase 1 gene (COI)—promises a rapid, accurate means of identifying animals to a species level. This study establishes that sequence variability in the barcode region permits discrimination of 98% of 201 fish species from the Canadian Pacific. The average sequence variation within species was 0.25%, while the average distance separating species within genera was 3.75%. The latter value was considerably lower than values reported in other studies, reflecting the dominance of the Canadian fauna by members of the young and highly diverse genus Sebastes. Although most sebastids possessed distinctive COI sequences, four species did not. As a partial offset to these cases, the barcode records indicated the presence of a new, broadly distributed species of Paraliparis and the possibility that Paraliparis pectoralis is actually a species pair. The present study shows that most fish species in Pacific Canadian waters correspond to a single, tightly cohesive array of barcode sequences that are distinct from those of any other species, but also highlights some taxonomic issues that need further investigation.

Supplementary material

227_2009_1284_MOESM1_ESM.pdf (855 kb)
(PDF 854 kb) A neighbour-joining tree of COI sequence divergences (K2P) in all 1,225 individuals of this study. Species names, BOLD process ID, Sample ID, sequence length, and numbers of ambiguous bases are given at branch tips
227_2009_1284_MOESM2_ESM.pdf (457 kb)
(PDF 456 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Dirk Steinke
    • 1
  • Tyler S. Zemlak
    • 1
    • 2
  • James A. Boutillier
    • 3
  • Paul D. N. Hebert
    • 1
  1. 1.Canadian Centre for DNA Barcoding, Biodiversity Institute of OntarioUniversity of GuelphGuelphCanada
  2. 2.Department of BiologyDalhousie UniversityHalifaxCanada
  3. 3.Pacific Biological StationFisheries and Oceans CanadaNanaimoCanada

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