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Marine Biology

, Volume 155, Issue 2, pp 211–221 | Cite as

Photosynthetic performance of giant clams, Tridacna maxima and T. squamosa, Red Sea

  • Carin Jantzen
  • Christian Wild
  • Mohammed El-Zibdah
  • Hilly Ann Roa-Quiaoit
  • Christoph Haacke
  • Claudio Richter
Original Paper

Abstract

Two species of giant clams, Tridacna maxima and T. squamosa, coexist in the Red Sea, but exhibit distinctly different depth distributions: T. maxima mostly occurs in shallow waters (reef flat and edge), while T. squamosa may occur down to the lower fore-reef slope. Giant clams have been described as mixotrophic, capable of both filter-feeding and photosynthesis due to algal symbionts (zooxanthellae), therefore, observed depth preferences were investigated in relation to possible differences in autotrophy vs. heterotrophy. This study was conducted from April to June 2004, at the reef near the Marine Science Station, Aqaba, Gulf of Aqaba, Red Sea, and in May 2007, at a reef near Dahab, Sinai Peninsula, Egypt. In situ measurements using a submersible pulse amplitude modulated fluorometer (Diving PAM), revealed no significant differences in effective PSII quantum yield (ΔF/Fm′) and relative electron transport rates (ETR) between the two species; but rapid light curves (ETR vs. light, photosynthetically active irradiance, PAR) showed significant differences in maximum photosynthetic rates (ETRmax), with 20% higher values in T. maxima. Chamber incubations displayed higher net and gross oxygen production by T. maxima (88.0 and 120.3 μmol O2 cm−2 mantle area day−1) than T. squamosa (56.7 and 84.8 μmol O2 cm−2 mantle area day−1); even under shading conditions (simulated depth of 20 m) T. maxima still achieved 93% of the surface gross O2 production, whereas T. squamosa reached only 44%. A correlation was found between ETR and net photosynthesis measured as oxygen production (T. maxima: R2 = 0.53; T. squamosa: R2 = 0.61). Calculated compensation depth (CD) (gross photosynthesis equals respiration) in T. maxima (16 m) matches the maximum depth of occurrence in this study (17 m). By contrast, the CD of T. squamosa (9 m) was much shallower than the maximum vertical range (42 m). Findings suggest T. maxima is a strict functional photoautotroph limited by light, whereas T. squamosa is a mixotroph whose photoautotrophic range is extended by heterotrophy.

Keywords

High Performance Liquid Chromatography Photosynthetically Active Radiation Reef Flat Electron Transport Rate Photosynthetic Performance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was funded by the German Ministry for Education and research (grant no. 03F0356A). Thanks are due to the scientific and technical staff of the MSS, in particular to Mohammed Rasheed, Yousef Ahmed and Abdullah Al-Momany (diving supervision) for support. Thanks to Matthias Birkicht and Stefani Bröhl for technical advice and logistics at the ZMT. Special thanks to Ralph Tollrian for supporting this study, especially the part in Dahab. Thanks to the MPI, notably Dirk deBeer and Raphaela Schoon for HPLC access and assistance.

Supplementary material

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References

  1. Ambariyanto, Hoegh-Guldberg O (1997) Effect of nutrient enrichment in the field on the biomass, growth and calcification of the giant clam Tridacna maxima. Mar Biol (Berl) 129:635–642. doi: 10.1007/s002270050206 CrossRefGoogle Scholar
  2. Baillie BK, Monje V, Silvestre V, Sison M, Belda-Baillie CA (1998) Allozyme electrophoresis as a tool for distinguishing different zooxanthellae symbiotic with giant clams. Proc R Soc Lond B Biol Sci 265:1949–1956. doi: 10.1098/rspb.1998.0525 CrossRefGoogle Scholar
  3. Beckvar N (1981) Cultivation, spawning and growth of the giant clams Tridacna gigas, Tridacna derasa and Tridacna squamosa in Palau, Caroline Islands. Aquaculture 24:21–30. doi: 10.1016/0044-8486(81)90040-5 CrossRefGoogle Scholar
  4. Beer S, Ilan M, Eshel A, Weil A, Brickner I (1998) Use of pulse amplitude modulated (PAM) fluorometry for in situ measurements of photosynthesis in two faviid corals. Mar Biol 131:607–612. doi: 10.1007/s002270050352 CrossRefGoogle Scholar
  5. Belda CA, Lucas JS, Yellowlees D (1993) Nutrient limitation in the giant-clam symbiosis: effects of nutrient supplements on growth of the symbiotic partners. Mar Biol (Berl) 117:655–664. doi: 10.1007/BF00349778 CrossRefGoogle Scholar
  6. Belda-Baillie CA, Sison M, Silvestre V, Villamor K, Monje V, Gomez ED et al (1999) Evidence for changing symbiotic algae in juvenile tridacnids. J Exp Mar Biol Ecol 241:207–221. doi: 10.1016/S0022-0981(99)00079-9 CrossRefGoogle Scholar
  7. Fisher CR, Fitt WK, Trench RK (1985) Photosynthesis and respiration in Tridacna gigas as a function of irradiance and size. Biol Bull 169:230–245. doi: 10.2307/1541400 CrossRefGoogle Scholar
  8. Fitt WK, Cook CB (2001) Photoacclimation and the effect of the symbiotic environment on the photosynthetic response of symbiotic dinoflagellates in the tropical marine hydroid Myrionema amboinense. J Exp Mar Biol Ecol 256:15–31. doi: 10.1016/S0022-0981(00)00302-6 CrossRefGoogle Scholar
  9. Gorbunov MY, Kolber ZS, Lesser MP, Falkowski PG (2001) Photosynthesis and photoprotection in symbiotic corals. Limnol Oceanogr 46:75–85CrossRefGoogle Scholar
  10. Griffiths DJ, Klumpp DW (1996) Relationships between size, mantle area and zooxanthellae numbers in five species of giant clam (Tridacnidae). Mar Ecol Prog Ser 137:139–147. doi: 10.3354/meps137139 CrossRefGoogle Scholar
  11. Griffiths DJ, Winsor H, Luong-Van T (1992) Iridophores in the mantle of giant clams. Aust J Zool 40:319–326. doi: 10.1071/ZO9920319 CrossRefGoogle Scholar
  12. Hawkins AJS, Klumpp DW (1995) Nutrition of the giant clam Tridacna gigas (L.) II. Relative contribution of filter feeding and the ammonium acquired and recycled by symbiotic algae towards total nitrogen requirements for tissue growth and metabolism. J Exp Mar Biol Ecol 190:263–290. doi: 10.1016/0022-0981(95)00044-R CrossRefGoogle Scholar
  13. Iglesias-Prieto R, Trench RK (1994) Acclimation and adaptation to irradiance in symbiotic dinoflagellates. I Responses of the photosynthetic unit to changes in photon flux. J Exp Mar Biol Ecol 113:163–175Google Scholar
  14. Ishikura M, Kato C, Maruyama T (1997) UV-absorbing substances in zooxanthellate and azooxanthellate clams. Mar Biol (Berl) 128:649–655. doi: 10.1007/s002270050131 CrossRefGoogle Scholar
  15. Jeffrey SW, Haxo FT (1967) Photosynthetic pigments of symbiotic dinoflagellates (zooxanthellae) from corals and clams. Biol Bull 135:149–165. doi: 10.2307/1539622 CrossRefGoogle Scholar
  16. Jeffrey SW, Mantoura RFC, Bjørnland T (1997) Data for the identification of 47 key phytoplankton pigments. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography, guidelines to modern methods, vol 10. UNESCO Publishing, Paris, pp 449–559Google Scholar
  17. Klumpp DW, Griffiths CL (1994) Contribution of phototrophic and heterotrophic nutrition to the metabolic and growth requirements of four species of giant clam (Tridacnidae). Mar Ecol Prog Ser 115:103–115. doi: 10.3354/meps115103 CrossRefGoogle Scholar
  18. Klumpp DW, Lucas JS (1994) Nutritional ecology of the giant clam Tridacna tevoroa and T. derasa from Tonga: influence of light on filter-feeding and photosynthesis. Mar Ecol Prog Ser 107:147–156. doi: 10.3354/meps107147 CrossRefGoogle Scholar
  19. Klumpp DW, Bayne BL, Hawkins AJS (1992) Nutrition of the giant clam Tridacna gigas (L.). I Contribution of filter feeding and photosynthates to respiration and growth. J Exp Mar Biol Ecol 155:105–122. doi: 10.1016/0022-0981(92)90030-E CrossRefGoogle Scholar
  20. Lesser MP (2000) Depth dependent photoacclimatization to solar ultraviolet radiation in the Caribbean coral Montastraea faveolata. Mar Ecol Prog Ser 192:137–151. doi: 10.3354/meps192137 CrossRefGoogle Scholar
  21. Lesser MP, Gorbunov MY (2001) Diurnal and bathymetric changes in chlorophyll fluorescence yields of reef corals measured in situ with a fast repetition rate fluorometer. Mar Ecol Prog Ser 212:69–77. doi: 10.3354/meps212069 CrossRefGoogle Scholar
  22. Mangum CP, Johansen K (1982) The influence of symbiotic dinoflagellates on respiratory processes in the giant clam Tridacna squamosa. Pac Sci 36:395–400Google Scholar
  23. Manu N, Sone S (1995) Breeding season of the Tongan shellfish. 3. Elongated giant clam, Tridacna maxima. Fish Res Bull Tonga 3:25–33Google Scholar
  24. Mobley KB, Gleason DF (2003) The effect of light and heterotrophy on the carotenoid concentrations in the Caribbean anemone Aiptasia pallida (Verrill). Mar Biol (Berl) 43:629–638. doi: 10.1007/s00227-003-1123-7 CrossRefGoogle Scholar
  25. Muscatine L (1990) The role of symbiotic algae in carbon and energy flux in reef corals. In: Dubinsky Z (ed) Coral reefs. Elsevier, AmsterdamGoogle Scholar
  26. Muscatine L, Porter JW (1977) Reef corals: mutualistic symbiosis adapted to nutrient-poor environments. Biomed Sci 27:454–460Google Scholar
  27. Norton JH, Shepherd MA, Long HM, Fitt WK (1992) The zooxanthellae tubular system in the giant clam. Biol Bull 183:503–506. doi: 10.2307/1542028 CrossRefGoogle Scholar
  28. Ralph PJ, Gademann R, Larkum AWD, Schreiber U (1999) In situ underwater measurements of photosynthetic activity of coral zooxanthellae and other reef-dwelling dinoflagellate endosymbionts. Mar Ecol Prog Ser 180:139–147. doi: 10.3354/meps180139 CrossRefGoogle Scholar
  29. Roa-Quiaoit H, Richter C (in review) Distribution, abundance, size and growth of giant clams (Tridacnidae) in the Northern Gulf of Aqaba, Red SeaGoogle Scholar
  30. Roa-Quiaoit H, Richter C, Zibdeh M (2004) Towards a sustainable aquaculture of giant clams (Tridacnidae) in the Jordanian sector of the Gulf of Aqaba. In: Sixth international aquarium congress, Monterey Bay Aquarium, Monterey, CA, USA, 5–10 December 2004Google Scholar
  31. Schreiber U (1986) Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer. Photosynth Res 9:261–272. doi: 10.1007/BF00029749 CrossRefGoogle Scholar
  32. Shick JM, Lesser MP, Dunlap WC, Stochaj WR, Chalker BE, Won JW (1995) Depth-dependent responses to solar ultraviolet radiation and oxidative stress in the zooxanthellate coral Acropora microphthalma. Mar Biol (Berl) 122:41–51. doi: 10.1007/BF00349276 CrossRefGoogle Scholar
  33. Sims N, Howard N (1988) Indigenous tridacnid clam populations and introduction of Tridacna derasa in the Cook Islands. In: Copland J, Lucas J (eds) Giant clams in Asia and the Pacific. ACIAR Monograph No. 9, ACIAR, Canberra, pp 34–40Google Scholar
  34. Streamer M, Griffiths DJ, Thinh L (1988) The products of photosynthesis by zooxanthellae (Symbiodinium microadriaticum) of Tridacna gigas and their transfer to the host. Symbiosis 6:237–252Google Scholar
  35. Titlyanov EA, Titlyanova TV, Yamazato K, van Woesik R (2001) Photo-acclimation dynamics of the coral Stylophora pistillata to low and extremely low light. J Exp Mar Biol Ecol 263:211–225. doi: 10.1016/S0022-0981(01)00309-4 CrossRefGoogle Scholar
  36. Trench RK (1979) Cell biology of plant-animal symbiosis. Annu Rev Plant Physiol Plant Mol Biol 30:485–531CrossRefGoogle Scholar
  37. Trench RK, Wethey DS, Porter JW (1981) Observations on the Symbiosis with zooxanthellae among the Tridacnidae (Mollusca, Bivalvia). Biol Bull 161:180–198. doi: 10.2307/1541117 CrossRefGoogle Scholar
  38. Warner ME, Chilcoat GC, McFarland FK, Fitt WK (2002) Seasonal fluctuations in the photosynthetic capacity of photosystem II in symbiotic dinoflagellates in the Caribbean reef-building coral Montastrea. Mar Biol (Berl) 141:31–38. doi: 10.1007/s00227-002-0807-8 CrossRefGoogle Scholar
  39. Wild C, Tollrian R, Huettel M (2004) Rapid recycling of coral mass-spawning products in permeable reef sediments. Mar Ecol Prog Ser 271:159–166. doi: 10.3354/meps271159 CrossRefGoogle Scholar
  40. Winkler LW (1888) The determination of dissolved oxygen in water. Ber Dtsch Chem Ges 21:2843–2857. doi: 10.1002/cber.188802102122 CrossRefGoogle Scholar
  41. Wright SW, Jeffrey SW (1997) High-resolution HPLC system for chlorophylls and carotenoids of marine phytoplankton. In: Jeffrey SW, Mantoura RFC, Wright SW (eds) Phytoplankton pigments in oceanography, guidelines to modern methods. UNESCO Publishing, ParisGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Carin Jantzen
    • 1
    • 2
  • Christian Wild
    • 2
  • Mohammed El-Zibdah
    • 3
  • Hilly Ann Roa-Quiaoit
    • 4
  • Christoph Haacke
    • 2
  • Claudio Richter
    • 5
  1. 1.Center for Tropical Marine Ecology (ZMT)BremenGermany
  2. 2.Coral Reef Ecology Work Group (CORE), GeoBio-Center and Department of Geosciences, Ludwig-Maximilians-UniversityMunichGermany
  3. 3.Marine Science Station AqabaThe University of Jordan and Yarmouk UniversityAqabaJordan
  4. 4.McKeough Marine CenterXavier UniversityCagayan de Oro CityPhilippines
  5. 5.Alfred-Wegener-Institute for Polar and Marine ResearchBremerhavenGermany

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