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Marine Biology

, Volume 153, Issue 4, pp 643–651 | Cite as

The fate of dietary lipids in the Arctic ctenophore Mertensia ovum (Fabricius 1780)

  • Martin Graeve
  • Marte Lundberg
  • Marco Böer
  • Gerhard Kattner
  • Haakon Hop
  • Stig Falk-Petersen
Research Article

Abstract

Lipids of the Arctic ctenophore Mertensia ovum, collected from Kongsfjorden (Svalbard) in 2001, were analysed to investigate seasonal variability and fate of dietary lipids. Total lipids, lipid classes and fatty acid and alcohol compositions were determined in animals, which were selected according to age-group and season. Changes in lipids of age-group 0 animals were followed during growth from spring to autumn. Total lipids increased from May to September. Lipids as percentage of dry mass were lowest in August indicating their use for reproduction. Higher values occurred in September, which may be due to lipid storage for overwintering. Wax esters were the major lipid class accounting for about 50% of total lipids in age-group 0 animals from July and August. Phospholipids were the second largest lipid fraction with up to 46% in this age-group. The principal fatty acids of M. ovum from all age-groups were 22:6(n-3), 20:5(n-3) and 16:0. Wax ester fatty alcohols were dominated by 22:1(n-11) and 20:1(n-9) followed by moderate proportions of 16:0. The unique feature of M. ovum lipids was the high amount of free fatty alcohols originating probably from the dietary wax esters. In May, free alcohols exhibited the highest mean proportion with 14.6% in age-group 0 animals. We present the first data describing a detailed free fatty alcohol composition in zooplankton. This composition was very different from the alcohol composition of M. ovum wax esters because of the predominance of the long-chain monounsaturated 22:1(n-11) alcohol accounting for almost 100% of total free alcohols in some samples. The detailed lipid composition clearly reflected feeding of M. ovum on the herbivorous calanoid species, Calanus glacialis and C. finmarchicus, the abundant members of the zooplankton community in Kongsfjorden. Other copepod species or prey items seem to be less important for M. ovum.

Keywords

Lipid Class Fatty Alcohol High Performance Thin Layer Chromatography Free Alcohol Major Lipid Class 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are grateful to the captain and crew of “Oceania”, RV Haakon Mosby and “Lance” and the staff of Kings Bay AS in Ny-Ålesund for their professional support during field experiments. We would like to thank Charlotte Gannefors, Anette Wold and Marthi Wolff for their important assistance. This work was supported by the Personnel Exchange Programme between the Research Council of Norway and Deutscher Akademischer Austauschdienst (DAAD).

References

  1. Albers CS, Kattner G, Hagen W (1996) The compositions of wax esters, triacylglycerols and phospholipids in Arctic and Antarctic copepods: evidence of energetic adaptations. Mar Chem 55:347–358CrossRefGoogle Scholar
  2. Basedow SL, Eiane K, Tverberg V, Spindler M (2004) Advection of zooplankton in an Arctic fjord (Kongsfjorden, Svalbard). Estuar Coast Shelf Sci 60:113–124CrossRefGoogle Scholar
  3. Böer M, Gannefors C, Kattner G, Graeve M, Hop H, Falk-Petersen S (2005) The Arctic pteropod Clione limacina: seasonal lipid dynamics and life-strategy. Mar Biol 147:707–717CrossRefGoogle Scholar
  4. Clarke A, Peck LS (1990) The physiology of polar marine zooplankton. Polar Res 10:355–369CrossRefGoogle Scholar
  5. Dalsgaard J, St John M, Kattner G, Müller-Navarra D, Hagen W (2003) Fatty acid trophic markers in the pelagic marine environment. Adv Mar Biol 46:225–340CrossRefGoogle Scholar
  6. Falk-Petersen S, Dahl TM, Scott CL, Sargent JR, Gulliksen B, Kwasniewski S, Hop H, Millar R-M (2002) Lipid biomarkers and trophic linkages between ctenophores and copepods in Svalbard waters. Mar Ecol Prog Ser 227:187–194CrossRefGoogle Scholar
  7. Folch J, Lees M, Stanley GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226:497–509Google Scholar
  8. Graeve M (1992) Umsatz und Verteilung von Lipiden in arktischen marinen Organismen unter besonderer Berücksichtigung unterer trophischer Stufen. Ber Polarforsch 124:1–140Google Scholar
  9. Graeve M, Kattner G, Hagen W (1994) Diet-induced changes in the fatty acid composition of Arctic herbivorous copepods: experimental evidence of trophic markers. J Exp Mar Biol Ecol 182:97–110CrossRefGoogle Scholar
  10. Granhag L, Norrbin F, Haanes H, Henriksen J, Kolb J (2005) Feeding preference of the Arctic ctenophore Mertensia ovum. ASLO Aquatic Sciences Meeting, Santiago de Compostella, SpainGoogle Scholar
  11. Hazel JR (1995) Thermal adaptation in biological membranes: is homeoviscous adaptation the explanation? Annu Rev Physiol 57:19–42CrossRefGoogle Scholar
  12. Hop H, Pearson T, Hegseth EN, Kovacs KM, Wiencke C, Kwasniewski S, Eiane K, Mehlum F, Gulliksen B, Wlodarska-Kowalczuk M, Lydersen C, Weslawski JM, Cochrane S, Gabrielsen GW, Leakey RJG, Lønne OJ, Zajaczkowski M, Falk-Petersen S, Kendall M, Wängberg S-Å, Bischof K, Voronkov AY, Kovaltchouk NA, Wiktor J, Poltermann M, di Prisco G, Papucci C, Gerland S (2002) The marine ecosystem of Kongsfjorden, Svalbard. Polar Res 21:167–208CrossRefGoogle Scholar
  13. Ju S-J, Scolardi K, Daly KL, Harvey HR (2004) Understanding the trophic role of the Antarctic ctenophore, Callinaria antarctica, using lipid biomarkers. Polar Biol 27:782–792CrossRefGoogle Scholar
  14. Kattner G, Fricke HSG (1986) Simple gas–liquid chromatographic method for the simultaneous determination of fatty acids and alcohols in wax esters of marine organisms. J Chromatogr 361:263–268CrossRefGoogle Scholar
  15. Kattner G, Graeve M (1991) Wax ester composition of the dominant calanoid copepods of the Greenland Sea/Fram Strait region. Polar Res 10:479–487CrossRefGoogle Scholar
  16. Kattner G, Krause M (1987) Changes in lipids during the development of Calanus finmarchicus s.l. from Copepodid I to adult. Mar Biol 96:511–518CrossRefGoogle Scholar
  17. Kattner G, Hirche H-J, Krause M (1989) Spatial variability in lipid composition of calanoid copepods from Fram Strait, the Arctic. Mar Biol 102:473–480CrossRefGoogle Scholar
  18. Kattner G, Hagen W, Graeve M, Albers C (1998) Exceptional lipids and fatty acids in the pteropod Clione limacina (Gastropoda) from both polar oceans. Mar Chem 61:219–228CrossRefGoogle Scholar
  19. Kwasniewski S, Hop H, Falk-Petersen S, Pedersen G (2003) Distribution of Calanus species in Kongsfjorden, a glacial fjord in Svalbard. J Plankton Res 25:1–20CrossRefGoogle Scholar
  20. Larson RJ, Harbison GR (1989) Source and fate of lipids in polar gelatinous zooplankton. Arctic 42:339–346CrossRefGoogle Scholar
  21. Lee RF, Hagen W, Kattner G (2006) Lipid storage in marine zooplankton. Mar Ecol Prog Ser 307:273–306CrossRefGoogle Scholar
  22. Leu E, Falk-Petersen S, Kwasniewski S, Wulf A, Edvardsen K, Hessen DO (2006) Fatty acid dynamics during the spring bloom in a high Arctic fjord: importance of abiotic factors versus community changes. Can J Fish Aquat Sci 63:2760–2779CrossRefGoogle Scholar
  23. Lundberg M, Hop H, Eiane K, Gulliksen B, Falk-Petersen S (2006) Population structure and accumulation of lipids in the ctenophore Mertensia ovum. Mar Biol 149:1344–1353CrossRefGoogle Scholar
  24. Nelson MM, Phleger CF, Mooney BD, Nichols PD (2000) Lipids of gelatinous Antarctic zooplankton: Cnidaria and Ctenophora. Lipids 35:551–559CrossRefGoogle Scholar
  25. Olsen RE, Henderson RJ (1989) The rapid analysis of neutral and polar marine lipids using double-development HPTLC and scanning densitometry. J Exp Mar Biol Ecol 129:189–197CrossRefGoogle Scholar
  26. Percy JA (1988) Influence of season, size, and temperature on the metabolism of an arctic cydippid ctenophore, Mertensia ovum (FABRICIUS). Sarsia 73:61–70CrossRefGoogle Scholar
  27. Percy JA (1989) Abundance, biomass, and size frequency distribution of an arctic ctenophore, Mertensia ovum (Fabricius) from Frobisher Bay, Canada. Sarsia 74:95–105CrossRefGoogle Scholar
  28. Percy JA, Fife FJ (1981) The biochemical composition and energy content of Arctic marine macrozooplankton. Arctic 34:307–313CrossRefGoogle Scholar
  29. Phleger CF, Nichols PD, Virtue P (1998) Lipids and trophodynamics of Antarctic zooplankton. Comp Biol Physiol 120B:311–323Google Scholar
  30. Raskoff KA, Purcell JE, Hopcroft RR (2005) Gelatinous zooplankton of the Arctic Ocean: in situ observations under the ice. Polar Biol 28:207–217CrossRefGoogle Scholar
  31. Sargent JR, Henderson RJ (1986) Lipids. In: Corner EDS, O’ Hara E (eds) Biological chemistry of marine copepods. University Press, Oxford, pp 59–108Google Scholar
  32. Scott CL, Kwasniewski S, Falk-Petersen S, Sargent JR (2000) Lipids and life strategy of Calanus finmarchicus, Calanus glacialis and Calanus hyperboreus in late autumn, Kongsfjorden, Svalbard. Polar Biol 23:510–516CrossRefGoogle Scholar
  33. Scott CL, Kwasniewski S, Falk-Petersen S, Sargent JR (2002) Species differences, origins and functions of fatty alcohols and fatty acids in the wax esters and phospholipids of Calanus hyperboreus, C. glacialis and C. finmarchicus from Arctic waters. Mar Ecol Prog Ser 235:127–134CrossRefGoogle Scholar
  34. Siferd TD, Conover RJ (1992) Natural history of ctenophores in the Resolute Passage area of the Canadian High Arctic with special reference to Mertensia ovum. Mar Ecol Prog Ser 86:133–144CrossRefGoogle Scholar
  35. Swanberg N, Båmstedt U (1991) Ctenophora in the Arctic: the abundance, distribution and predatory impact of the cydippid ctenophore Mertensia ovum (Fabricius) in the Barents Sea. Polar Res 10:507–524CrossRefGoogle Scholar
  36. Tande KS, Henderson RJ (1988) Lipid composition of copepodite stages and adult females of Calanus glacialis in Arctic waters of the Barents Sea. Polar Biol 8:333–339CrossRefGoogle Scholar
  37. Walkusz W, Storemark K, Skau T, Gannefors C, Lundberg M (2003) Zooplankton community structure; a comparison of fjords, open water and ice stations in the Svalbard area. Pol Polar Res 24:149–165Google Scholar
  38. Willis K, Cottier F, Kwasniewski S, Wold A, Falk-Petersen S (2006) The influence of advection on zooplankton community composition in an Arctic fjord (Kongsfjorden, Svalbard). J Mar Syst 61:39–54CrossRefGoogle Scholar
  39. Wold A, Leu E, Walkusz W, Falk-Petersen S (2007) Lipids in copepodite stages of Calanus glacialis. Polar Biol 30:655–658CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Martin Graeve
    • 1
  • Marte Lundberg
    • 2
  • Marco Böer
    • 1
  • Gerhard Kattner
    • 1
  • Haakon Hop
    • 2
  • Stig Falk-Petersen
    • 2
  1. 1.Alfred Wegener Institute for Polar and Marine ResearchBremerhavenGermany
  2. 2.Norwegian Polar InstituteTromsøNorway

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