mtDNA control region and D-HPLC analysis: a method to evaluate the mating system in Syngnathidae (Teleostei)
- 121 Downloads
Mitochondrial DNA (mtDNA) has been used extensively in animals for phylogenetic and population studies but it has been applied only occasionally to mating systems analysis, where nuclear markers are usually preferred. We used denaturing-high performance liquid chromatography (D-HPLC) to detect mitochondrial DNA polymorphisms to assess the genetic mating system in Syngnathus abaster. Our study of ten pregnant males, revealed polygyny in 30% of the males, who carried embryos originating from multiple females. In addition, 30% of the pregnant males carried embryos with the same mitochondrial haplotype. This is not sufficient to demonstrate polyandry but allows a rapid selection and a reduced sample size for further studies. In conclusion, the proposed technique is time- and cost-effective, allows the certain identification of polygyny and provides useful information to study polyandry.
KeywordsTriethylammonium Acetate Intrapopulation Variability Pregnant Male Genetic Mating System Apical Embryo
We thank Francesco Cucca and Daniela Contu (Dipartimento di Scienze Biomediche e Biotecnologie, Università di Cagliari) for help in D-HPLC analysis, and Tiziana Lai for useful discussion on DNA extraction. This work was supported by Interreg III and MURST (ex-60%) grants to MC and PF.
- Breder CM Jr, Rosen DE (1966) Modes of reproduction in fishes. Natural History Press, New YorkGoogle Scholar
- Carcupino M, Baldacci A, Mazzini M, Franzoi P (2002) Functional significance of the male brood pouch in the reproductive strategies of pipefishes and seahorses: a morphological and ultrastructural comparative study on three anatomically different pouches. J Fish Biol 61:1465–1480CrossRefGoogle Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp 41:95–98Google Scholar
- Semino O, Passarino G, Oefner PJ, Lin AA, Arbuzova S, Beckman LE, De Benedictis G, Francalacci P, Kouvatsi A, Limborska S, Marcikiae M, Mika A, Mika B, Primorac D, Santachiara-Benerecetti AS, Cavalli-Sforza LL, Underhill PA (2000) The genetic legacy of Paleolithic Homo sapiens in extant Europeans: a Y chromosome perspective. Science 290:1155–1159CrossRefGoogle Scholar
- Tomasini JA, Quignard JP, Capapé C, Bouchereau JL (1991) Facteurs du success reproductif de Syngnathus abaster Risso, 1826 (Pisces, Teleostei, Syngnathidae) en milieu lagunaire méditerranéen (lagune de Mauguio, France). Acta Oecol 12:331–355Google Scholar
- Underhill PA, Shen P, Lin AA, Jin L, Passarino G, Yang WH, Kauffman E, Hurlbut A, Bonné-Tamir B, Bertranpetit J, Francalacci P, Ibrahim M, Jenkins T, Kidd JR, Mehdi SQ, Seielstad MT, Wells RS, Piazza A, Soergel D, Sherry S, Feldman MW, Davis RW, Cavalli-Sforza LL, Oefner PJ (2000) Y Chromosome variation and the history of human populations. Nat Genet 26:358–361CrossRefGoogle Scholar