Advertisement

Marine Biology

, Volume 151, Issue 6, pp 2153–2158 | Cite as

Genetic diversity of blackspot seabream (Pagellus bogaraveo) populations off Spanish Coasts: a preliminary study

  • J. A. Piñera
  • G. Blanco
  • E. Vázquez
  • J. A. Sánchez
Research Article

Abstract

Genetic diversity among four natural samples of Blackspot seabream (Pagellus bogaraveo, Brünnich, 1768) from different fishing grounds exploited by Spanish fisheries was analyzed through the use of 12 microsatellite markers. The samples were captured off the Spanish coasts from the Mediterranean Sea to the Cantabrian Sea within the same continental slope. High levels of genetic diversity were revealed for every population and every locus was polymorphic at the 0.95 level. The average number of alleles, average heterozygosity and PIC were found to be 15.75, 0.833 and 0.818, respectively. In general, population differentiation was not detected in these samples. Through AMOVA, a low level of variation between regions (Mediterranean vs. Atlantic samples) was observed, though this was not significant. A larger percentage of total variation was observed inside the ‘within populations’ class. Thus, AMOVA did not reveal any significant population substructure. Moreover, no correlation was found between geographical and FST estimates and the observed results did not allow the improvement of a model of isolation by distance. The high homogeneity revealed by means of these markers could indicate the absence of physical frontiers between the geographical areas analyzed in this survey, especially between Atlantic and Mediterranean areas.

Keywords

Microsatellite Locus Continental Slope Allelic Richness Atlantic Area Spanish Coast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study is part of a multidisciplinary project partially funded by JACUMAR. J.A. Piñera’s work is supported by a Ficyt grant from the “Gobierno del Principado de Asturias”.

We would like to thank Yaisel Borrell, Miguel Corujo, Agustín Fernández and David Bernardo for their friendship and their constant help.

References

  1. Aboim MA, Menezes GM, Schlitt T, Rogers AD (2005) Genetic structure and history of populations of the deep-sea fish Helicolenus dactylopterus (Delaroche, 1809) inferred from mtDNA sequence analysis. Mol Ecol 14:1343–1354CrossRefGoogle Scholar
  2. Alarcón JA, Magoulas A, Georgakopoulos T, Zorros E, Álvarez MC (2004) Genetic comparison of wild and cultivated european populations of the gilthead seabream (Sparus aurata). Aquaculture 230:65–80CrossRefGoogle Scholar
  3. Bargelloni L, Alarcón JA, Álvarez MC, Penzo E, Magoulas A, Reis C, Patarnello T (2003) Discord in the family Sparidae (Teleostei): divergent phylogeographical patterns across the Atlantic-Mediterranean divide. J Evol Biol 16:1149–1158CrossRefGoogle Scholar
  4. Borsa P, Blanquer A, Berrebi P (1997) Genetic structure of the flounders Platichthys flesus and P stellatus at different geographic scales Mar Biol 129:233–246CrossRefGoogle Scholar
  5. Carvalho GR, Hauser L (1995) Molecular genetics and the stock concept in fisheries. In: Carvalho GR, y Pitcher TJ (eds) Molecular genetics in fisheries. Chapman & Hall, Londres, pp 55–80CrossRefGoogle Scholar
  6. Estoup A, Angers B (1998) Microsatellites and minisatellites for molecular ecology: theoretical and empirical consideration. In: Carvalho GR (ed) Advances in molecular ecology. IOS Press, pp. 55–86Google Scholar
  7. Excoffier L, Smouse PE, Quattro JM (1995) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491Google Scholar
  8. FAO (2004) The States of world Fisheries and Aquaculture (SOFIA). Available on web: http://www.fao.org/documents/show_cdr.asp?url_file=/DOCREP/007/y5600e/y5600e00.htm
  9. Féral JP (2002) How useful are the genetic markers in attemps to understand and manage biodiversity? J Exp Mar Biol Ecol 268:121–145CrossRefGoogle Scholar
  10. García de León FJ, Dallas JF, Chatain B, Cannone M, Versini JL, Bonhomme F (1995) Development and use of microsatellite markers in sea bass Dicentrarchus labrax (Linnaeus, 1758) (Perciformes: Serranidae). Mol Mar Biol Biotechnol 4:62–68Google Scholar
  11. García de León FJ, Chikhi L, Bonhomme F (1997) Microsatellite polymorphism and population subdivision in natural populations of European sea bass Dicentrarchus labrax. Mol Ecol 6:51–62CrossRefGoogle Scholar
  12. Goodman SJ (1997) RST CALC: A collection of computer programs for calculating unbiased estimates of genetic differentiation and determining their significance for microsatellite data. Mol Ecol 6:881–885CrossRefGoogle Scholar
  13. Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html
  14. Hanel R, Sturmbauer C (2000) Multiple recurrent evolution of trophic types in Northeastern Atlantic and Mediterranean Seabreams (Sparidae, Percoidei). J Mol Evol 50:276–283CrossRefGoogle Scholar
  15. Hedgecock D (1986) Is gene flow from pelagic larval dispersal important in adaptation and evolution of marine invertebrates? Bull Mar Sci 39:550–564Google Scholar
  16. Hureau JC, Monod Th (1973) Check-list of the fishes of the north-eastern Atlantic and Mediterranean. Unesco Publication, Paris, pp 426–443Google Scholar
  17. Macpherson E (1998) Ontogenic shifts in habitat use and aggregation in juvenile sparid fishes. J Exp Mar Biol Ecol 220:127–150CrossRefGoogle Scholar
  18. Micale V, Maricchiolo G, Genovese L (2002) The reproductive biology of blackspot seabream Pagellus bogaraveo in captivity. I. gonadal development, maturation and hermaphroditism. J Appl Ichthyol 18:172–176CrossRefGoogle Scholar
  19. Naciri M, Lemaire C, Borsa P, Bonhomme F (1999) Genetic study of the Atlantic/Mediterranean transition in sea bass (Dicentrarchus labrax). J Hered 90:591–596CrossRefGoogle Scholar
  20. Oosterhout CV, Hutchinson WF, Wills PM, Shipley P (2004) micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes.  doi:10.1111/j.1471-8286.2004.00684.x CrossRefGoogle Scholar
  21. Orrell TM, Carpenter KE (2004) A phylogeny of the fish family Sparidae (porgies) inferred from mitochondrial sequence data. Mol Phylogenet Evol (in press)Google Scholar
  22. Palumbi SR (1994) Genetic divergence, reproductive isolation and marine speciation. Annu Rev Ecol Syst 25:547–572CrossRefGoogle Scholar
  23. Piñera JA, Bernardo D, Blanco G, Vázquez E, Sánchez JA (2006) Isolation and characterization of polymorphic microsatellite markers in Pagellus bogaraveo, and cross-species amplification in Sparus aurata and Dicentrarchus labrax. Mol Ecol Notes 6:33–35CrossRefGoogle Scholar
  24. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249CrossRefGoogle Scholar
  25. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225CrossRefGoogle Scholar
  26. Rousset F (1996) Equilibrium values of measures of population subdivision for stepwise mutation patterns. Genetics 142:1357–1362PubMedPubMedCentralGoogle Scholar
  27. Schneider S, Roessli D, Excoffier L (2000) Arlequin: software for population genetics data analysis. Ver. 2000. Genetics and Biometry Lab. Dept of Anthropology, University of GenevaGoogle Scholar
  28. Slatkin M (1995) A measure of population subdivision based on microsatellite allele frequencies. Genetics 139:457–462PubMedPubMedCentralGoogle Scholar
  29. Sousa F, Isidro E, Erzini K (1999) Semi-pelagic longline selectivity for two demersal species from the Azores: the black spot sea bream (Pagellus bogaraveo) and the bluemouth rockfish (Helicolenus dactylopterus dactylopterus). Fish Res 41:25–35CrossRefGoogle Scholar
  30. Stamatis C, Triantafyllidis A, Moutou A, Mamuris Z (2004) Mitochondrial DNA variation in Northeast Atlantic and Mediterranean populations of Norway lobster, Nephrops norvegicus. Mol Ecol 13:1377–1390CrossRefGoogle Scholar
  31. Stockley BM, Rogers AD, Iyengar A, Menezes G, Santos R, Long A (2000) Ten microsatellite loci isolated and developed for the blackspot seabream, Pagellus bogaraveo (Brünnich 1768). Mol Ecol 9:99–1000CrossRefGoogle Scholar
  32. Stockley BM, Menezes G, Pinho MR, Rogers AD (2005) Genetic population structure in the black-spot sea bream (Pagellus bogaraveo Brünnich, 1768) from the NE Atlantic. Mar Biol 146:793–804CrossRefGoogle Scholar
  33. Swofford DL, Selander B (1989) BIOSYS-1: a FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. J Hered 72:282–302Google Scholar
  34. Thorpe JP, Solé-Cava AM, Watts PC (2000) Exploited marine invertebrates: genetics and fisheries. Hydrobiologia 420:165–184CrossRefGoogle Scholar
  35. Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513PubMedGoogle Scholar
  36. Ward RD (2000) Genetics in fisheries management. Hydrobiologia 420:191–201CrossRefGoogle Scholar
  37. Zardoya R, Castillo R, Grande C, Favre-Krey L, Caetano S, Marcato S, Krey G, Patarnello T (2004) Differential population structuring of two closely related fish species, the mackerel (Scomber scombrus) and the chub mackerel (Scomber japonicus), in the Mediterrenean Sea. Mol Ecol 13:1785–1798CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • J. A. Piñera
    • 1
  • G. Blanco
    • 1
  • E. Vázquez
    • 1
  • J. A. Sánchez
    • 1
  1. 1.Laboratorio de Genética Acuícola, Área de Genética, Departamento de Biología FuncionalUniversidad de OviedoOviedoSpain

Personalised recommendations