Phylogeographic structure of Octopus vulgaris in South Africa revisited: identification of a second lineage near Durban harbour
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In a previous study that investigated genetic structure of Octopus vulgaris along the South African coast by sequencing the mitochondrial cytochrome oxidase III gene (COIII), all sequences generated were identical. Such a finding is unusual, because mitochondrial DNA mutates quickly, and several marine invertebrates present in southern Africa show considerable genetic variation and structure. We reanalysed the samples using two different mitochondrial markers, namely cytochrome oxidase I (COI) and the large ribosomal subunit (16S rRNA). Sequences of both these markers showed variation. The conclusion of the previous study, that South Africa’s O. vulgaris population is characterised by a lack of genetic structure along the coast, is rejected. Some specimens from Durban (southeast Africa) were genetically more different from those found in the remainder of the country than were specimens from other regions (Tristan da Cunha and Senegal). We suggest that the lineage in Durban may have been recently introduced.
KeywordsBallast Water South African Population Relative Rate Test Squid Species Fourfold Degenerate Site
We are grateful to Francisco Santaclara (Vigo, Spain) for providing tissue samples from Spain and Senegal, to James Glass for providing samples from Tristan da Cunha, and to Pierre Pradervand for providing samples from Durban and Umhlanga. We also thank three anonymous referees for some useful comments that considerably improved the quality of this manuscript. This study was supported by a Claude Harris Leon Foundation postdoctoral research fellowship awarded to PRT, Rhodes University and an NRF grant to NPB (GUN 2069119). The experiments conducted in this study comply with current South African laws.
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotech 3:294–299Google Scholar
- Klopper AW (2005) Intraspecific genetic variation in the percoid teleosts Argyrosomus japonicus (Temminck and Schlegel, 1843) and Pomadasys commersonnii (Lacepède, 1801) as inferred from the mitochondrial control region. M. Sc. thesis, University of Pretoria, South AfricaGoogle Scholar
- Norton O (2006) The population structure of two estuarine fish species along the southern African coastline. M. Sc. Thesis, Rhodes University, South AfricaGoogle Scholar
- Oosthuizen A, Jiwaji M, Shaw P (2004) Genetic analysis of the Octopus vulgaris population on the coast of South Africa. S Afr J Sci 100:603–607Google Scholar
- Palumbi SR (1996) Nucleic acids II: the polymerase chain reaction. In: Hillis DM, Moritz C, Mable BK (eds) Molecular systematics. Sinauer Associates, Sunderland, pp 205–247Google Scholar
- Warnke K (1999) Diversität des Artenkomplexes Octopus cf. vulgaris Cuvier, 1797 in Beziehung zu seiner Verbreitung an der Ost- und Westküste Lateinamerikas. PhD thesis, Universität Bremen, Shaker Verlag, AachenGoogle Scholar
- Zardi GI, McQuaid CD, Teske PR, Barker NP (2007) Unexpected genetic structure in indigenous (Perna perna) and invasive (Mytilus galloprovincialis) mussel populations in South Africa. Mar Ecol Prog Ser (in press)Google Scholar