Patterns and UV sensitivity of carbon anhydrase and nitrate reductase activities in south Pacific macroalgae
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This study describes the activities of the key enzymes involved in carbon incorporation (carbonic anhydrase, CA) and inorganic nitrogen reduction (nitrate reductase, NR) in 25 intertidal macroalgae of southern Chile (39°S). UV radiation as a factor affecting the nutrient metabolism of algae was also examined. The results of the enzyme activities and the UV sensitivity were related to the position of the algae on the shore, species/taxonomic groups and morpho-functional patterns. The CA activity in the studied algae ranged from 42 to 165 REA g−1 FW, and was neither related to growing depth nor to taxonomic or morpho-functional groups. The NR activities ranged from 0.1 to 8.9 μmol NO 2 − g−1 FW min−1, with the highest levels observed in red algae. In contrast to CA, the NR activities showed a decreasing tendency from supra/midlittoral to infra/sublittoral. Also, differences between morpho-functional groups were seen. The impact of artificial UV radiation on CA and NR activities was variable as in some species it provoked an increase while in other species a decrease was observed, suggesting species-specific responses and UV sensitivity. The CA activity was the most UV sensitive in the taxonomic group Chlorophyta and in the supralittoral algae. The UV sensitivity of NR activity could not be related to any patterns related to morpho-functional or taxonomic groups and habitat depth.
KeywordsCarbonic Anhydrase Macroalgae Nitrate Reductase Nitrate Reductase Activity Carbonic Anhydrase Activity
This study was funded by CONICYT-Chile (FONDECYT 7050062 to I.G.) and the Academy of Finland (202398 to P.H.).
- Arcos D, Peña H, Núñez S, Ortiz J, Furet L, Figueroa S, Sepúlveda A, Rebolledo H, Castillo J, Turner A, González H, Valenzuela G, Menschel E (2000) Determinación de la capacidad de carga de las zonas estuarinas de los Rios Valdivia y Bueno (X Región). Valdivia. Technical Report FIP 2000-29, Instituto de Investigación Pesquera, Talcahuano, ChileGoogle Scholar
- Badger MR (1987) The CO2 concentration mechanism in aquatic phototrophs. In: Hatch MD, Boardman NK (eds) The biochemistry of plants: a comprehensive treatise, vol 10. Academic, San Diego, pp 219–274Google Scholar
- Corzo A, Niell FX (1994) Nitrate–reductase activity and in vivo nitrate-reduction rate in Ulva rigida illuminated by blue light. Mar Biol 120:17–23Google Scholar
- Figueroa FL, Viñegla B (2001) Effects of solar UV radiation on photosynthesis and enzyme activities (carbonic anhydrase and nitrate reductase) in marine macroalgae from southern Spain. Rev Chi Hist Nat 74:237–249Google Scholar
- Flores-Moya A, Gómez I, Viñegla B, Altamirano M, Pérez-Rodríguez E, Maestre C, Caballero RM, Figueroa FL (1998) Effects of solar radiation on the endemic Mediterranean red alga Rissoella verruculosa: photosynthetic performance, pigment content and the activities of enzymes related to nutrient uptake. New Phytol 139:673–684 CrossRefGoogle Scholar
- Sinha RP, Singh N, Kumar A, Kumar HD, Häder M, Häder DP (1995) Effects of UV irradiation on certain physiological and biochemical processes in cyanobacteria. J Photochem Photobiol B Biol 30:107–113 Google Scholar
- Snell FD, Snell CT (1949) Colorimetric methods of analysis, 3rd edn. Van Norstrand, Princeton, p 804Google Scholar
- Thomas TE, Harrison PJ (1988) A comparison of in vitro and in vivo nitrate reductase assays in three intertidal seaweeds. Bot Mar 31:101–107Google Scholar
- Wilbur KM, Anderson NG (1948) Electrometric and colorimetric determination of carbonic anhydrase. J Biol Chem 30:541–547Google Scholar