Influence of temperature and salinity on the trace element incorporation into statoliths of the common cuttlefish (Sepia officinalis)
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The use of statolith chemistry to trace migration pathways and distinguish populations of cephalopods is based on the assumption that the elemental composition of statoliths is influenced by physicochemical properties of the ambient environment. However, such influences have not been investigated experimentally up until now. This study presents the first microchemical analyses of cephalopod statoliths obtained from laboratory experiments under different controlled temperature and salinity conditions. Our results show that statolith chemical composition is strongly related to both salinity and temperature in ambient waters. The Ba/Ca ratio is negatively related to temperature and shows no relation to salinity. The I/Ca ratio is positively related to temperature and negatively to salinity. No Sr/Ca relation was found to either salinity or temperature, suggesting that the well-established proxy strontium is not as useful in cephalopod statoliths as in other biomineralized aragonites. Microanalysis of trace elements, however, shows an enormous potential for field studies on distribution, migration and stock separation of cephalopods. Furthermore, Synchrotron X-ray Fluorescence Analysis is introduced as a promising novel method for statolith analysis, providing a spatial resolution of typically 10–15 μm combined with detection limits down to 0.5 ppm.
KeywordsStrontium Aragonite Fish Otolith Strontianite Japanese Common Squid
Thanks are due to Frank Lechtenberg for invaluable help with analytical and quantification procedures. Armelle Perrin and Juergen Beusen assisted in collecting Sepia eggs. The Kiel Aquarium team provided technical and logistical support during the experiments. This work was funded by the Deutsche Forschungsgemeinschaft (DFG PI 203/11-1, HA 2100/9-1, PI 203/11-2, PI 203-3).
- Espen PJ van, Nullens H, Adams F (1977) A computer analysis of X-ray fluorescence spectra. Nucl Instrum Methods 142:269–273Google Scholar
- FAO (2005) FISHSTAT plus global data set capture production 1950–2003 (FAO yearbook Fishery Statistics). ftp://www.ftp.fao.org/fi/stat/windows/fishplus/capdet.zip
- Gallahar NK, Kingsford MJ (1996) Factors influencing Sr/Ca ratios in otoliths of Girella elevata: an experimental investigation. J Fish Biol 48:174–186Google Scholar
- Grieken RE van, Markowicz AA (1993) Handbook of X-ray spectrometry. Marcel Dekker, New York, Basel, Hongkong, p 704Google Scholar
- Kalish JM (1990) Use of otolith microchemistry to distinguish the progeny of sympatric anadromous and non-anadromous salmonids. Fish Bull US 88:657–666Google Scholar
- Kristensen TK (1980) Periodical growth rings in cephalopod statoliths. Dana 1:39–51Google Scholar
- Knezovich JP (1994) Chemical and biological factors affecting bioavailability of contaminants in seawater. In: Hamelink JL, Landrum PF, Bergman HL, Benson WH (eds) Bioavailability: physical, chemical and biological interactions. Lewis Publishers, London, pp 23–30Google Scholar
- Lechtenberg F, Garbe S, Bauch J, Dingwell DB, Freitag F, Haller M, Hansteen TH, Ippach P, Knöchel A, Radtke M, Romano C, Sachs PM, Schmincke HU, Ullrich HJ (1996) The X-ray fluorescence measurement place at beamline L of Hasylab. J Trace Microprobe Tech 14(3):561–587Google Scholar
- Lipinski MR (1993) The deposition of statoliths: a working hypothesis. In: Okutani T, O’Dor RK, Kubodera T (eds) Recent advances in cephalopod fisheries biology. Tokai University Press, TokyoGoogle Scholar
- Zumholz K, Klügel A, Hansteen TH, Piatkowski U (2007a) Statolith microchemistry traces environmental history of the boreoatlantic armhook squid Gonatus fabricii. Mar Ecol Progr Ser (in press)Google Scholar
- Zumholz K, Hansteen TH, Hillion F, Horreard F, Piatkowski U (2007b) Elemental distribution in cephalopod statoliths: NanoSIMS provides new insights into nanoscale structure. Rev Fish Biol Fisher (in press)Google Scholar