Marine Biology

, Volume 151, Issue 2, pp 719–727 | Cite as

High self-recruitment levels in a Mediterranean littoral fish population revealed by microsatellite markers

  • Josep Carreras-Carbonell
  • Enrique Macpherson
  • Marta Pascual
Research Article


Self-recruitment rates are essential parameters in the estimation of connectivity among populations, having important consequences in marine conservation biology. Using ten highly polymorphic microsatellite loci, we estimate, over 3 years, the self-recruitment in a population of Tripterygion delaisi in the NW Mediterranean. Six previously described source populations were used for the assignment (Costa Brava, Columbretes, Formentera, Cabo de Palos, Cabo de Gata and Tarifa). Even though this species has a 16–21 day larval duration, a mean of 66.4 ± 1.4% of the recruits settled in their natal population. When refining in a more local scale the origin of individuals self-recruited to Costa Brava, using as source the three sampling localities that conform this population (Cap de Creus, Tossa and Blanes), the highest percentage (40.6 ± 8.9%) was self-assigned to the adult source locality (Blanes) where recruits were sampled each year. Our results suggest that a high proportion of the larvae of T. delaisi remained close to, or never leave, their natal spawning area. This observation can be extrapolated to other species with similar early life-history traits and low adult mobility and can have important implications for the conservation and management of Mediterranean littoral fishes.


Genetic Differentiation Source Population Assignment Test Sequential Bonferroni Procedure Planktonic Larval Duration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank S. Planes his helpful comments. This research was supported by a Predoctoral fellowship from the Ministerio de Educación, Cultura y Deporte to J.C. (AP2001-0225). Research was funded by projects CTM2004-05265 and BOS2003-05904 of the MCYT and MMA 119/2003. Researchers are part of the SGR 2005SGR-00995 and 2005SGR-00277 of the Generalitat de Catalunya. All the experiments made comply with the current Spanish laws.


  1. Appleyard SA, Grewe PM, Innes BH, Ward RD (2001) Population structure of yellowfin tuna (Thunnus albacares) in the western Pacific Ocean, inferred from microsatellite loci. Mar Biol DOI 10.1007/s002270100578Google Scholar
  2. Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (2004) GENETIX 4.05, logiciel sous Windows TM pour la génétique des populations. Laboratoire Génome, Populations, Interactions, CNRS UMR 5171, Université de Montpellier II, Montpellier (France)Google Scholar
  3. Blaxter JHS (1986) Development of sense organs and behaviour of teleost larvae with special reference to feeding and predator avoidance. Trans Am Fish Soc 115:98–114CrossRefGoogle Scholar
  4. Caley MJ, Carr MH, Hixon MA, Hughes TP, Jones GP, Menge BA (1996) Recruitment and the local dynamics of open marine populations. Annu Rev Ecol Syst 27:477–500CrossRefGoogle Scholar
  5. Carlsson J, McDowell JR, Díaz-James P (2004) Microsatellite and mitochondrial DNA analyses of Atlantic bluefin tuna (Thunnus thynnus thynnus) population structure in the Mediterranean Sea. Mol Ecol DOI 10.1111/j.1365–294X.2004.02336.xGoogle Scholar
  6. Carreras-Carbonell J, Macpherson E, Pascual M (2004) Isolation and characterization of microsatellite loci in Tripterygion delaisi. Mol Ecol Notes DOI 10.1111/j.1471-8286.2004.00688.xGoogle Scholar
  7. Carreras-Carbonell J, Macpherson E, Pascual M (2006) Population structure within and between subspecies of the Mediterranean triplefin fish Tripterygion delaisi revealed by highly polymorphic microsatellite loci. Mol Ecol DOI 10.1111/j.1365-294X.2006.03003.xGoogle Scholar
  8. Cornuet JM, Piry S, Luikart G, Estoup A, Solignac M (1999) New methods employing multilocus genotypes to select or exclude populations as origins of individuals. Genetics 153:1989–2000PubMedGoogle Scholar
  9. Cowen RK (2002) Larval dispersal and retention and consequences for population connectivity. In: Sale P (ed) Coral reef fishes; diversity and dynamics in a complex ecosystem. Academic, San Diego, pp 149–170Google Scholar
  10. Cowen RK, Paris CB, Srinivasan A (2006) Scaling of connectivity in marine populations. Science DOI 10.1126/science.1122039Google Scholar
  11. Cushing DH (1996) Towards a science of recruitment in fish populations. Ecology Institute, OldendorfGoogle Scholar
  12. Estoup A, Largiadèr CR, Perrot E, Chourrout D (1996) Rapid one–tube DNA extraction for reliable pcr detection of fish polymorphic markers and transgenes. Mol Mar Biol Biotechnol 5:295–298Google Scholar
  13. Genton BJ, Shykoff JA, Giraud T (2005) High genetic diversity in French invasive populations of common ragweed, Ambrosia artemisiifolia, as a result of multiple sources of introduction. Mol Ecol DOI 10.1111/j.1365-294X.2005.02750.xGoogle Scholar
  14. Guo SW, Thompson EA (1992) Performing the exact test for Hardy-Weinberg proportions for multiple alleles. Biometrics 48:361–372PubMedCrossRefGoogle Scholar
  15. Heymer A (1977) Expériences subaquatiques sur les performances d’orientation et de retour au gite chez Tripterygion tripteronotus et Tripterygion xanthosoma (Blennioidei, Tripterygiidae). Vie et Milieu 3e sér 27:425–435Google Scholar
  16. Hoarau G, Rijnsdorp AD, Van der Veer HW, Stam WT, Olsen JL (2002) Population structure of plaice (Pleuronectes platessa l.) in northern Europe: microsatellites revealed large-scale spatial and temporal homogeneity. Mol Ecol DOI 10.1046/j.1365-294X.2002.01515.xGoogle Scholar
  17. Jones GP, Milicich MJ, Emslie MJ, Lunow C (1999) Self-recruitment in a coral reef fish population. Nature DOI 10.1038/45538Google Scholar
  18. Jones GP, Planes S, Thorrold SR (2005) Coral reef fish larvae settle close to home. Curr Biol DOI 10.1016/j.cub.2005.06.061Google Scholar
  19. Kingsford MJ, Leis JM, Shanks A, Lindeman KC, Morgan SG, Pineda J (2002) Sensory environments, larval abilities and local self-recruitment. Bull Mar Sci 70:309–340Google Scholar
  20. Klanten OS, Choat JH, van Herwerden L (2006) Extreme genetic diversity and temporal rather than spatial partitioning in a widely distributed coral reef fish. Mar Biol DOI 10.1007/s00227-006-0372-7Google Scholar
  21. Knutsen H, André C, Jorde PE, Skogen MD, Thuróczy E, Stenseth NC (2004) Transport of North Sea cod larvae into the Skagerrak coastal populations. Proc R Soc Lond B DOI 10.1098/rspb.2004.2721Google Scholar
  22. Largier JL (2003) Considerations in estimating larval dispersal distances from oceanographic data. Ecol Appl 13:S71–S89Google Scholar
  23. Leis J, McCormick M (2002) The biology, behaviour and ecology of the pelagic, larval stage of coral reef fishes. In: Sale P (ed) Coral reef fishes; diversity and dynamics in a complex ecosystem. Academic, San Diego, pp 171–200Google Scholar
  24. Leis JM (1991) The pelagic stages of reef fishes: the larval biology of coral reef fishes. In: Sale PF (ed) The ecology of fishes on coral reefs. Academic, San Diego, pp 183–230Google Scholar
  25. Leis JM, Carson-Ewart BM (eds) (2000) The larvae of Indo-Pacific coastal fishes: an identification guide to marine fish larvae, 1st edn. Brill, BostonGoogle Scholar
  26. Lloret J, Palomera I, Salat J, Sole I (2004) Impact of freshwater input and wind on landings of anchovy (Engraulis encrasicolus) and sardine (Sardina pilchardus) in shelf waters surrounding the Ebre (Ebro) River delta (north-western Mediterranean). Fish Oceanogr DOI 10.1046/j.1365-2419.2003.00279.xGoogle Scholar
  27. Macpherson E, Raventós N (2006) Relationships between pelagic larval duration and geographic distribution in Mediterranean littoral fishes. Mar Ecol Prog Ser (in press)Google Scholar
  28. Miller JA, Shanks AL (2004) Evidence for limited larval dispersal in black rockfish (Sebastes melanops): implications for population structure and marine-reserve design. Can J Fish Aquat Sci DOI 10.1139/F04-111Google Scholar
  29. Paetkau D, Slade R, Burden M, Estoup A (2004) Genetic assignment methods for the direct, real-time estimation of migration rate: a simulation-based exploration of accuracy and power. Mol Ecol DOI 10.1046/j.1365-294X.2004.02008.xGoogle Scholar
  30. Patterson HM, Kingsford MJ, McCulloch MT (2005) Resolution of the early life history of a reef fish using otolith chemistry. Coral Reefs DOI 10.1007/s00338-004-0469–8Google Scholar
  31. Piry S, Alapetite A, Cornuet JM, Paetkau D, Baudouin L, Estoup A (2004) GENECLASS2: a software for genetic assignment and first-generation migrant detection. J Hered DOI 10.1093/jhered/esh074Google Scholar
  32. Planes S (2002) In: Sale PF (ed) The ecology of fishes on coral reefs. Academic, San Diego, pp 201–220Google Scholar
  33. Rannala B, Mountain JL (1997) Detecting immigration by using multilocus genotypes. Proc Natl Acad Sci USA 94:9197–9201PubMedCrossRefGoogle Scholar
  34. Raventós N, Macpherson E (2001) Planktonic larval duration and settlement marks on the otoliths of Mediterranean littoral fishes. Mar Biol DOI 10.1007/s002270000535Google Scholar
  35. Raventós N, Macpherson E (2005) Environmental influences on temporal patterns of settlement in two littoral labrid fishes in the Mediterranean Sea. Estuar Coast Shelf Sci DOI 10.1016/j.ecss.2004.11.018Google Scholar
  36. Raymond M, Rousset F (1995) GENEPOP: Population genetics software for exact tests and ecumenism, version 1.2. J Hered 86:248–249Google Scholar
  37. Rice WR (1989) Analysing tables of statistical tests. Evolution 43:223–225CrossRefGoogle Scholar
  38. Rico C, Turner GF (2002) Extrem microallopatric divergence in a cichlid species from Lake Malawi. Mol Ecol DOI 10.1046/j.1365-294X.2002.01537.xGoogle Scholar
  39. Riginos C, Victor BC (2001) Larval spatial distributions and other early life-history characteristics predict genetic differentiation in eastern Pacific blennioid fishes. Proc R Soc Lond B 268:1931–1936CrossRefGoogle Scholar
  40. Roberts CM (1997) Connectivity and management of Caribbean coral reefs. Science DOI 10.1126/science.278.5342.1454Google Scholar
  41. Rocha LA, Robertson DR, Roman J, Bowen BW (2005) Ecological speciation in tropical reef fishes. Proc R Soc Lond B 272:573–579CrossRefGoogle Scholar
  42. Sabatés A, Zabala M, Garcia-Rubies A (2003) Larval fish communities in the Medes Islands marine reserve (north-west Mediterranean). J Plankton Res 25:1035–1046Google Scholar
  43. Sala E, Aburto-Oropeza O, Paredes G, Parra I, Barrera JC, Dayton PK (2002) A general model for designing networks of marine reserves. Science DOI 10.1126/science.1075284Google Scholar
  44. Sale PF (1991) Reef fish communities: open nonequilibrial systems. In: Sale PF (ed) The ecology of fishes on coral reefs. Academic, San Diego, pp 564–598Google Scholar
  45. Shanks AL, Eckert G (2005) Population persistence of California Current fishes and benthic crustaceans: a marine drift paradox. Ecol Monogr 75:505–524Google Scholar
  46. Shulman MJ, Bermingham E (1995) Early life histories, ocean currents, and the population genetics of Caribbean reef fishes. Evolution 49:897–910CrossRefGoogle Scholar
  47. Swearer SE, Caselle JE, Lea DW, Warner RR (1999) Larval retention and recruitment in an island population of a coral-reef fish. Nature DOI 10.1038/45533Google Scholar
  48. Swearer SE, Shima JS, Hellberg ME, Thorrold SR, Jones GP, Robertson DR, Morgan SG, Selkoe KA, Ruiz GM, Warner RR (2002) Evidence of self-recruitment in demersal marine populations. Bull Mar Sci 70:251–271Google Scholar
  49. Thorrold SR, Latkoczy C, Swart PK, Jones CM (2001) Natal homing in a marine fish metapopulation. Science DOI 10.1126/science.291.5502.297Google Scholar
  50. Thorrold SR, Jones GP, Hellberg ME, Burton RS, Swearer SE, Neigel JE, Morgan SG, Warner RR (2002) Quantifying larval retention and connectivity in marine populations with artificial and natural markers. Bull Mar Sci 70:291–308Google Scholar
  51. Tintoré J, Wang DP, García E, Viúdez A (1995) Near inertial motions in the coastal ocean. J Mar Sys 6:301–312CrossRefGoogle Scholar
  52. Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370CrossRefGoogle Scholar
  53. Wilson DT, Meekan MG (2001) Environmental influences on patterns of larval replenishment in coral reef fishes. Mar Ecol Prog Ser 222:197–208Google Scholar
  54. Wirtz P (1978) The behaviour of the Mediterranean Tripterygion species (Pisces, Blennioidei). Zeitschrift für Tierpsychologie 48:142–174CrossRefGoogle Scholar
  55. Wright S (1951) The genetical structure of populations. Ann Hum Genet 15:323–354Google Scholar
  56. Zander CD (1986) Tripterygiidae. In: Whitehead PJP, Bauchot ML, Hureau JC, Nielsen J, Tortonese E (eds) Fishes of the North-Eastern Atlantic and the Mediterranean, vol 3. Unesco, Paris, pp 1118–1121Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Josep Carreras-Carbonell
    • 1
    • 2
  • Enrique Macpherson
    • 1
  • Marta Pascual
    • 2
  1. 1.Centre d’Estudis Avançats de Blanes, Consejo Superior de Investigaciones Científicas (CEAB-CSIC)BlanesSpain
  2. 2.Genetics DepartmentUniversity of BarcelonaBarcelonaSpain

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