Marine Biology

, Volume 151, Issue 2, pp 629–638 | Cite as

Age, growth and population structure of Modiolus barbatus from the Adriatic

  • M. PehardaEmail author
  • C. A. Richardson
  • I. Mladineo
  • S. Šestanović
  • Z. Popović
  • J. Bolotin
  • N. Vrgoč
Research Article


Age, growth and population structure of Modiolus barbatus from Mali Ston Bay, Croatia were determined using modal size (age) classes in length frequency distributions, annual pallial line scars on the inner shell surface, internal annual growth lines in shell sections of the middle nacreous layer and Calcein marked and transplanted mussels. The length frequency distributions indicated that M. barbatus attain a length of ∼40 mm in 5–6 years indicating that a large proportion of the population in Mali Ston Bay is <5 years old. Some mussels of ∼60 mm were predicted to be 14 years old using the Von Bertalanffy growth (VBG) equation. Up to the first 6 pallial line scars were visible in young (<6 years) mussels but in older shells the first scars became obscured by nacre deposition as the mussel increased in length and age. The age of the older shells (>6 years) was determined from the middle nacreous lines in shell section, which formed annually in winter between February and March; the wider dark increments forming during summer (June to September). The oldest mussel, determined from the middle nacreous lines, was >12 years, with the majority of mussels aged between 3 and 6 years of age. The ages of mussels ascertained using the growth lines were not dissimilar to the ages predicted from the length frequency distributions. Age at length curves produced using modal size class data were not different from the data obtained using the pallial scar rings and internal growth lines. Taken together these data suggest that M. barbatus attains a length of 40 and 50 mm within 5 and 8 years, respectively. Eighty one percent of individual M. barbatus injected with a Calcein seawater solution (300 mg Calcein l−1), into their mantle cavity successfully deposited a fluorescent line, which was visible in suitably prepared shell sections under ultra violet light. Incorporation of Calcein into the mussel shells was seasonally variable with the lowest frequency of incorporation in mussels marked in February and recovered in May. Seasonal shell growth was observed with significantly higher growth rates in mussels marked in May and removed in August (ANCOVA, F3,149 = 23.11, P < 0.001). Mussels (∼18 to 22 mm) marked in May and recovered in August displayed maximal growth rates of >2.5 mm month−1 compared with a mean mussel growth rate of 1.2 ± 0.6 mm month−1. At other times of the year mussel shell growth ranged from immeasurable to 1.48 mm month−1.


Calcein Growth Line Shell Growth Mussel Shell Length Frequency Distribution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was financed by the Croatian Ministry of Science and Technology. The authors are grateful to Željko Baće, Marko Žarić, Nika Stragličić, Lovorka Kekez and Mark Prime for technical assistance. Special thanks to Barbara Zorica for help with statistical analysis and Professor C.D. McQuaid for helpful suggestions for Calcein marking the mussel shells. The experiments conducted comply with the current laws of Republic of Croatia.


  1. Anwar NA, Richardson CA, Seed R (1990) Age determination, growth rate and population structure of the horse mussel Modiolus modiolus. J Mar Biol Assoc UK 70:441–457CrossRefGoogle Scholar
  2. Benović A (1997) The history, present condition, and future of the molluscan fisheries of Croatia. In: MacKenzie CL Jr, Burrell VG Jr, Rosenfield A, Hobart WL (eds) The history, present condition, and future of the molluscan fisheries of north and Central America and Europe, vol 3, Europe. NOAA Technical Report NMFS 129. US Department of Commerce, pp 217–226Google Scholar
  3. Bhattacharya CG (1967) A simple method of resolution of a distribution into Gaussian components. Biometrics 23:115–135CrossRefGoogle Scholar
  4. Dame RF (1996) Ecology of marine bivalves—an ecosystem approach. CRC Press Inc, Boca RatonCrossRefGoogle Scholar
  5. Gaspar MB, Richardson CA, Monteiro CA (1994) The effects of dredging on shell formation in the razor clam, Ensis siliqua from Barrinha, southern Portugal. J Mar Biol Assoc UK 74:927–938Google Scholar
  6. Gosling E (2003) Bivalve mollusks—biology, ecology and culture. Fishing news books, OxfordGoogle Scholar
  7. Gulland JA, Holt SJ (1959) Estimation of growth parameters for data of unequal time intervals. J Cons Int Explor Mer 25:47–49CrossRefGoogle Scholar
  8. Gray AP, Lucas IAN, Seed R, Richardson CA (1999) Mytilus edulis chilensis infested with Coccomyxa parasitica (Chlorococcales, Coccomyxaceae). J Moll Stud 65:289–294CrossRefGoogle Scholar
  9. Jones DS, Williams DF, Arthur MA (1983) Growth history and ecology of the Atlantic surf clam Spisula solidissima (Dillwyn), as revealed by stable isotopes and annual shell increments. J Exp Mar Biol Ecol 73:225–242CrossRefGoogle Scholar
  10. Kaehler S, McQuaid CD (1999a) Use of the fluorochrome Calcein as an in situ growth marker in the brown mussel Perna perna. Mar Biol 133:455–460CrossRefGoogle Scholar
  11. Kaehler S, McQuaid CD (1999b) Lethal and sub-lethal effects of phototrophic endoliths attacking the shell of the intertidal mussel Perna perna. Mar Biol 135:497–503CrossRefGoogle Scholar
  12. Kennedy H, Richardson CA, Duarte CM, Kennedy P (2001) Oxygen and carbon stable isotopic profiles of the fan mussel Pinna nobilis, and reconstruction of sea surface temperatures in the Mediterranean. Mar Biol 139:1115–1124CrossRefGoogle Scholar
  13. Krantz DE, Jones DS, Williams DF (1984) Growth rates of the sea scallop, Placopecten magellanicus determined from the 18O/16O record in shell calcite. Biol Bull 167:186–199CrossRefGoogle Scholar
  14. Leontarkis P, Richardson CA (2005) Growth of the smooth clam, Callista chione (Linnaeus, 1758) (Bivalvia: Veneridae) from the Thracian Sea, NE Mediterranean: morphometric relationships, annual shell banding, age and growth rate. J Moll Stud 71:189–192CrossRefGoogle Scholar
  15. Mancha Zeichen M, Agnesi S, Mariani A, Maccaroni A, Ardizzone GD (2002) Biology and population dynamics of Donax trunculus L. (Bivalvia: Donacidae) in the south Adriatic coast (Italy). Estuar Coast Shelf Sci 54:971–982CrossRefGoogle Scholar
  16. Mladineo I, Peharda M, Orhanović S, Bolotin J, Pavela-Vrančić M, Treursić B (2007) The reproductive cycle, condition index and biochemical composition of Modiolus barbatus. Helgoland Mar Res (submitted)Google Scholar
  17. Morton B (1977) The biology and functional mortality of Modiolus metcalfei (Bivalvia, Mytilacea) from the Singapore mangrove. Malacologia 16:501–517Google Scholar
  18. Peharda M, Richardson C, Onofri V, Bratoš A, Crnčević M (2002) Age and growth of Noah’s Ark shell, Arca noae L., in the Croatian Adriatic Sea. J Moll Stud 68:307–310CrossRefGoogle Scholar
  19. Peharda M, Morton B (2006) Experimental prey species preferences of Hexaplex trunculus (Gastropoda: Muricidae) and predator–prey interactions with the Black mussel Mytilus galloprovincialis (Bivalvia: Mytilidae). Mar Bio 148:1011–1019CrossRefGoogle Scholar
  20. Poppe GT, Goto Y (2000) European seashells, vol II (Scaphopoda, Bivalvia, Cephalopoda), 2nd edn. ConchBooks, Hackenheim GermanyGoogle Scholar
  21. Ramsy K, Richardson CA (2000) Techniques for assessing repaired shell damage in dog cockles, Glycymeris glycymeris L. J Shellfish Res 19:927–931Google Scholar
  22. Richardson CA (2001) Molluscs as archives of environmental change. Oceanogr Mar Biol Ann Rev 39:103–164Google Scholar
  23. Richardson CA, Kennedy HA, Duarte CM, Kennedy P, Proud SV (1999) Age and growth of the fan mussel Pinna nobilis from S.E. Spanish Mediterranean seagrass, Posidonia oceanica meadows. Mar Biol 133:205–212CrossRefGoogle Scholar
  24. Richardson CA, Peharda M, Kennedy HA Kennedy P, Onofri V (2004) Age, growth rate and season of recruitment of Pinna nobilis in the Croatian Adriatic determined from Mg:Ca and Sr:Ca shell profiles. J Exp Mar Biol Ecol 299:1–16CrossRefGoogle Scholar
  25. Seed R, Brown RA (1978) Growth as a strategy for survival in two marine bivalves, Cerastoderma edule and Modiolus modiolus. J Anim Ecol 47:283–292CrossRefGoogle Scholar
  26. Sparre P, Venema SC (1992) Introduction to tropical fish stock assessment. Part 1, Manual. FAO Fish Tech Pap (Revision 1) RomeGoogle Scholar
  27. Tumandara MI Jr, Yap HT, McManus LT, Ingles JA, López MG (1997) Growth, mortality and recruitment pattern of the brown mussel, Modiolus metcalfei (Bivalvia: Mytilacea), in Panguil Bay, Southern Philippines. Aquaculture 154:233–245CrossRefGoogle Scholar
  28. Zavodnik D (1997) Non-conventional seafood sources at the eastern Adriatic sea markets. In: Finka B (ed) Thousand years from first mention of fisheries in Croats (in Croatian). Croatian Academy of Arts and Sciences, Zagreb, pp 637–656Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • M. Peharda
    • 1
    Email author
  • C. A. Richardson
    • 2
  • I. Mladineo
    • 1
  • S. Šestanović
    • 1
  • Z. Popović
    • 1
  • J. Bolotin
    • 3
  • N. Vrgoč
    • 1
  1. 1.Institute of Oceanography and FisheriesSplitCroatia
  2. 2.School of Ocean ScienceUniversity of Wales – BangorAngleseyUK
  3. 3.Institute for Marine and Coastal ResearchUniversity of DubrovnikDubrovnikCroatia

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