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Marine Biology

, Volume 151, Issue 1, pp 219–232 | Cite as

Spatial and temporal variation in the elemental and stable isotopic content of the seagrasses Posidonia oceanica and Cymodocea nodosa from the Illes Balears, Spain

  • J. W. Fourqurean
  • N. Marbà
  • C. M. Duarte
  • E. Diaz-Almela
  • S. Ruiz-Halpern
Research Article

Abstract

Morphology, elemental content and isotopic composition of leaves of the seagrasses Posidonia oceanica and Cymodocea nodosa were highly variable across the Illes Balears, a Spanish archipelago in the western Mediterranean, and varied seasonally at one site in the study area. The data presented in this paper generally expand the reported ranges of nitrogen, phosphorus, iron and arsenic content and δ13C and δ15N for these species. Nitrogen and phosphorus content of P. oceanica leaves also showed significant seasonal variability; on an annual basis, P. oceanica leaves averaged 1.55% N and 0.14% P at this monitoring site. Both N and P were more concentrated in the leaves in winter than in summer, with winter maxima of 1.76% N and 0.17% P and summer minima of 1.34% N and 0.11% P. There was no significant annual pattern observed in the δ13C of P. oceanica leaves, but there was a repeated 0.6‰ seasonal fluctuation in δ15N. Mean annual δ15N was 4.0‰; δ15N was lowest in May and it increased through the summer and autumn to a maximum in November. Over the geographic range of our study area, there were interspecific differences in the carbon, nitrogen and phosphorus content of the two species. Posidonia oceanica N:P ratios were distributed around the critical value of 30:1 while the ratios for C. nodosa were lower than this value, suggesting P. oceanica we collected was not consistently limited by N or P while C. nodosa tended toward nitrogen limitation. Nutrient content was significantly correlated to morphological indicators of plant vigor. Fe content of P. oceanica leaves varied by a factor of 5×, with a minimum of 31.1 μg g−1 and a maximum of 167.7 μg g−1. Arsenic was present in much lower tissue concentrations than Fe, but the As concentrations were more variable; the maximum concentration of 1.60 μg g−1 was eight times as high as the minimum of 0.20 μg g−1. There were interspecific differences in δ13C of the two species; C. nodosa was consistently more enriched (δ13C = −7.8 ± 1.7‰) than P. oceanica (−13.2 ± 1.2‰). The δ13C of both species decreased significantly with increasing water depth. Depth related and regional variability in the δ13C and δ15N of both species were marked, suggesting that caution needs to be exercised when applying stable isotopes in food web analyses.

Keywords

Phosphorus Content Light Availability Leaf Length Carbonate Sediment Interspecific Difference 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by the project M&M’s of the European Commission (contract #EVK3-CT-2000-00044) and grant SAB2000-0082 from the Secretaría de Estado de Educación y Universidades, Ministerio de Educación, Cultura y Deporte, Spain were instrumental in the field sampling effort. Yong Cai at FIU provided instruments and assistance for the Fe and As analyses. The Stable Isotope Lab at FIU analyzed the samples for stable isotopic ratios. Rocío Santiago, Regino Martínez, Susie Escorcia, Meredith Ferdie and Leanne Rutten helped with field sampling and sample analysis. This is contribution #335 of the Southeast Environmental Research Center at Florida International University. Collection of samples and laboratory analyses were conducted in accordance with the laws of Spain and the United States.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • J. W. Fourqurean
    • 1
  • N. Marbà
    • 2
  • C. M. Duarte
    • 2
  • E. Diaz-Almela
    • 2
  • S. Ruiz-Halpern
    • 1
  1. 1.Department of Biological Sciences and Southeast Environmental Research CenterFlorida International UniversityMiamiUSA
  2. 2.IMEDEA (CSIC-UIB), Instituto Mediterráneo de Estudios AvanzadosEsporlesSpain

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