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Marine Biology

, Volume 150, Issue 6, pp 1365–1377 | Cite as

The effect of hypoxia on behavioural and physiological aspects of lesser sandeel, Ammodytes tobianus (Linnaeus, 1785)

  • Jane W. Behrens
  • John F. Steffensen
Research Article

Abstract

Lesser sandeel (Ammodytes tobianus) is abundant in near-shore areas where it is a key prey. It exhibits the behaviour of alternating between swimming in schools and lying buried in the sediment. We first determined the species’ standard metabolic rate (SMR), critical partial pressure of oxygen \((P_{{\rm O}_{2{\rm crit}}})\) and maximal oxygen uptake \((M_{{\rm O}_{2{\rm max}}}).\) The sandeel were then exposed to an acute stepwise decline in water oxygen pressure (18.4, 13.8, 9.8, 7.5, 5.8, 4.0, and 3.1 kPa \(P_{{\rm O}_{2}}\)). Swimming speed and routine- and post-experimental blood lactate levels were measured, in addition to levels associated with strenuous exercise. The SMR was 69.0  ± 8.4 mg O2 kg−1 h−1 and the \(M_{{\rm O}_{2{\rm max}}}\) about seven times as high. The \(P_{{\rm O}_{2{\rm crit}}}\) was found to be 4.1 kPa. A rapid decrease (within 1 h) in \(P_{{\rm O}_{2}}\) from 18.4 to 3.1 kPa had no significant effect on routine swimming speed (0.9  ± 0.06 bl s−1), but steady levels at the lowest \(P_{{\rm O}_{2}}\) (3.1 kPa) gradually reduced the swimming speed by 95% after 40 min. The routine blood lactate levels were 2.2  ± 0.6 mmol l−1, while the levels in the strenuously exercised groups were significantly higher with 5.4  ± 1.6 and 5.8  ± 1.3 mmol l−1. The highest levels were observed in post-experimental fish with 7.5  ± 2.7 mmol l−1. We argue that, as sandeel showed no decrease in swimming speed (to offset stress) nor an increased speed to escape the hypoxia, the fish either rely on a low SMR and being a reasonable strong oxygen regulator \((\hbox{low}\;P_{{\rm O}_{2{\rm crit}}})\) as a mean to cope when exposed to acute hypoxia, or that the hypoxia simply developed too fast for the fish to decide on an appropriate strategy. Not showing a behavioural response may in the present case be maladaptive, as the consequence was major physiological stress which the fish however appears tolerant towards. The high routine blood lactate levels suggest that anaerobic metabolism is associated with swimming in sandeel, which may be related to the specific lifestyle of the fish where they regularly bury in the sediment.

Keywords

Blood Lactate Swimming Speed White Muscle Experimental Tank Acute Hypoxia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

\(P_{{\rm O}_{2{\rm crit}}}\)

Critical partial pressure of oxygen

\(M_{{\rm O}_{2{\rm max}}}\)

maximal oxygen uptake

MS

metabolic scope

\(P_{{\rm O}_{2}}\)

partial pressure of oxygen

SMR

Standard metabolic rate

Notes

Acknowledgements

We thank Dr. Neill Herbert, University of Glasgow, for assistance with the experimental design. The study was financially supported by a Ph.D. stipend from the Copenhagen Global Change Initiative (COGCI) Ph.D. School to Jane W. Behrens. The experiments comply with the Danish laws on animal ethics.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Marine Biological LaboratoryUniversity of CopenhagenHelsingørDenmark

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