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Marine Biology

, Volume 150, Issue 6, pp 1103–1113 | Cite as

Inter-specific and geographical variations in the fatty acid composition of mangrove leaves: implications for using fatty acids as a taxonomic tool and tracers of organic matter

  • T. MezianeEmail author
  • S. Y. Lee
  • P. L. Mfilinge
  • P. K. S. Shin
  • M. H. W. Lam
  • M. Tsuchiya
Research Article

Abstract

Fatty acid compositions of the leaves of six species of mangroves were studied to ascertain their use as biomarkers for determining the fate of mangrove organic matter in the habitat and as taxonomic tool. Mangrove leaves were collected from three locations in the western Pacific Ocean: Moreton Bay (MB) (Australia), Hong Kong (China) and Okinawa (Japan). In MB, samples were collected from two sites separated by 15 km: Logan River Estuary (LRE) and Jabiru Island. In addition, along the LRE, leaves were collected from five stations at ∼2–3 km apart. Results show that the analysis of the entire fatty acid profiles of the mangrove leaves is a promising taxonomic tool as the profiles of most species were sufficiently different to be separated in an non-metric multidimensional scaling plot. In addition, geographically separated populations of the same species could also be identified by their fatty acid profiles. In most cases, two non-ubiquitous groups of fatty acids dominated in the mangrove leaves: the polyunsaturated 18:2ω6 and 18:3ω3 and the long chain fatty acids (≥24:0). With respect to the relative contributions of these fatty acids, three groups of species were identified, in which one or both groups of fatty acids may potentially be used as markers of the mangrove organic matter in the estuarine environment.

Keywords

Fatty Acid Composition Total Fatty Acid Fatty Acid Profile Mangrove Species Total Fatty Acid Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by an Australian Research Council Discovery Project grant to SYL and TM (DP0344546). We thank Ms Alice Ho for competent technical help in GC-MS analysis of the Hong Kong samples and two anonymous reviewers. Associate Professor Yujuan Chen (Zhongshan University, China) helped establishing some of the protocols for GC analysis.

References

  1. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefGoogle Scholar
  2. Budge SM, Parrish CC (1998) Lipid biogeochemistry of plankton settling matter and sediments in Trinity Bay, Newfoundland. II. Fatty acids. Org Geochem 29:1547–1559CrossRefGoogle Scholar
  3. Budge SM, Iverson SJ, Bowen WD, Ackman RG (2002) Among- and within-species variability in fatty acid signatures of marine fish and invertebrates on the Scotian Shelf, Georges Bank, and southern Gulf of St. Lawrence. Can J Fish Aquat Sci 59:886–898CrossRefGoogle Scholar
  4. Chen J, Ferris H, Scow KM, Graham KJ (2001) Fatty acid composition and dynamics of selected fungal-feeding nematodes and fungi. Comp Biochem Physiol B 130:135–144CrossRefGoogle Scholar
  5. Clarke KR, Warwick RM (2001) Change in marine communities: an approach to statistical analysis and interpretation, 2nd edn. PRIMER-E, PlymouthGoogle Scholar
  6. Dalsgaard J, St John M, Kattner G, Muller-Navarra D, Hagen W (2003) Fatty acid trophic markers in the pelagic marine environment. Adv Mar Biol 46:225–340CrossRefGoogle Scholar
  7. Dodd RS, Rafii ZA, Fromard F, Blasco F (1998) Evolutionary diversity among Atlantic coast mangroves. Acta Oecol 19(3):323–330CrossRefGoogle Scholar
  8. Duke NC, Benzie JAH, Goodall JA, Ballment ER (1998) Genetic structure and evolution of species in the mangrove genus Avicennia (Avicenniacae) in the Indo-West Pacific. Evolution 56(6):1612–1626CrossRefGoogle Scholar
  9. Duke NC, Ying Lo EY, Sun M (2002) Global distribution and genetic discontinuities of mangroves—emerging patterns in the evolution of Rhizophora. Trees 16:65–79CrossRefGoogle Scholar
  10. Ellison AM (2002) Macroecology of mangroves: large-scale patterns and processes in tropical coastal forests. Trees 16:181–194CrossRefGoogle Scholar
  11. Freites L, Labarta U, Fernández-Reiriz M (2002) Evolution of fatty acid profiles of subtidal and rocky shore mussel seed (Mytilus galloprovincialis, Lmk.). Influence of environmental parameters. J Exp Mar Biol Ecol 268:185–204CrossRefGoogle Scholar
  12. Ge XJ, Sun M (1999) Reproductive biology and genetic diversity of a cryptoviviparous mangrove Aegiceras corniculatum (Myrsinaceae) using allozyme and intersimple sequence repeat (ISSR) analysis. Mol Ecol 8:2061–2069CrossRefGoogle Scholar
  13. Graeve M, Kattner G, Wiencke C, Kartsen U (2002) Fatty acid composition of Arctic and Antarctic macroalgae: indicator of phylogenetic and trophic relationship. Mar Ecol Prog Ser 231:67–74CrossRefGoogle Scholar
  14. Hogarth PJ (1999) The biology of mangroves. Oxford University Press, New York p 228Google Scholar
  15. Howell KL, Pond DW, Billet DSM, Tyler PA (2003) Feeding ecology of deep-sea seastars (Echinodermata: Asteroidea): a fatty acid biomarker approach. Mar Ecol Prog Ser 255:193–206CrossRefGoogle Scholar
  16. Kathiresan K, Bingham BL (2001) Biology of mangroves and mangroves ecosystems. Adv Mar Biol 40:81–251CrossRefGoogle Scholar
  17. Kunst L, Samuels AL (2003) Biosynthesis and secretion of plant cuticular wax. Prog Lipid Res 42:51–80CrossRefGoogle Scholar
  18. Lee SY (1989) Litter production and turnover of the mangrove Kandelia candel (L.) Druce in a Hong Kong tidal shrimp pond. Estuar Coast Shelf Sci 29:75–87CrossRefGoogle Scholar
  19. Lee SY (1997) Potential trophic importance of the faecal material of the mangrove sesarmine crab Sesarma messa. Mar Ecol Prog Ser 159:275–284CrossRefGoogle Scholar
  20. Mackey AP, Smail G (1995) Spatial and temporal variation in litter fall of Avicennia marina (Forssk.) Vierh. in the Brisbane River, Queensland, Australia. Aquat Bot 52:133–142CrossRefGoogle Scholar
  21. Melville F, Burchett M, Pulkownik A (2004) Genetic variation among age-classes of the mangrove Avicennia marina in clean and contaminated sediments. Mar Pollut Bull 49:695–703CrossRefGoogle Scholar
  22. Meziane T, Tsuchiya M (2000) Fatty acids as tracers of organic matter in the sediment and food web of a mangrove/intertidal flat ecosystem, Okinawa, Japan. Mar Ecol Prog Ser 200:49–57CrossRefGoogle Scholar
  23. Meziane T, Tsuchiya M (2002) Organic matter in a subtropical mangrove estuary subjected to wastewater discharge: origin and utilization by 2 macrozoobenthic species. J Sea Res 47:1–11CrossRefGoogle Scholar
  24. Meziane T, d’Agata F, Lee SY (2006) Fate of mangrove organic matter along a subtropical estuary: small-scale exportation and contribution to the food of crab communities. Mar Ecol Prog Ser 312:15–27CrossRefGoogle Scholar
  25. Mfilinge PL, Atta N, Tsuchiya M (2002) Nutrient dynamics and leaf litter decomposition in a subtropical mangrove forest at Oura Bay, Okinawa, Japan. Trees 16:172–180CrossRefGoogle Scholar
  26. Mfilinge PL, Meziane T, Bachok Z, Tsuchiya M (2003) Fatty acids in decomposition mangrove leaves: microbial activity, decay and nutritional quality. Mar Ecol Prog Ser 265:97–105CrossRefGoogle Scholar
  27. Mfilinge PL, Meziane T, Bachok Z, Tsuchiya M (2005) Litter dynamics and particulate organic matter outwelling from a subtropical mangrove in Okinawa Island, South Japan. Estuar Coast Shelf Sci 60:301–313CrossRefGoogle Scholar
  28. Napolitano GE, Pollero RJ, Gayoso AM (1997) Fatty acids as trophic markers of phytoplankton blooms in the Bahia Blanca estuary (Buenos Aires, Argentina) and Trinity Bay (Newfoundland, Canada). Biochem Syst Ecol 25:739–755CrossRefGoogle Scholar
  29. Robertson AI, Alongi DM, Boto KG (1992) Food chains and carbon fluxes. In: Robertson AI, Alongi DM (eds) Tropical mangrove ecosystems. American Geophysical Union, Washington, pp. 293–326CrossRefGoogle Scholar
  30. Schmit JP, Shearer CA (2004) Geographic and host distribution of lignicolous mangrove microfungi. Botanica Marina 47:496–500CrossRefGoogle Scholar
  31. Sheaves M, Molony B (2000) Short-circuit in the mangrove food chain. Mar Ecol Prog Ser 199:97–109CrossRefGoogle Scholar
  32. Smallwood BJ, Wooller MJ, Jacobson ME, Fogel ML (2003) Isotopic and molecular distributions of biochemicals from fresh and buried Rhizophora mangle leaves. Geochem Trans 4:38–46CrossRefGoogle Scholar
  33. Sun M, Wong KC, Lee JSY (1998) Reproductive biology and population genetic structure of Kandelia candel (Rhizophoraceae), a viviparous mangrove species. Am J Bot 85(11):1631–1637CrossRefGoogle Scholar
  34. Tan FX, Huang YL, Ge XJ, Su GH, Ni XW, Shi SH (2005) Population genetic structure and conservation implications of Ceriops decandra in Malay Peninsula and North Australia. Aquat Bot 81(2):175–188CrossRefGoogle Scholar
  35. Wannigama GP, Volkman JK, Gillan FT, Nichols PD, RB Johns (1981) A comparison of lipid components of the fresh and dead leaves and pneumatophores of the mangrove Avicennia marina. Phytochemistry 20(4):659–666CrossRefGoogle Scholar
  36. Xu J, Lin P, Meguro S, Kawachi S (1997) Phytochemical research on mangrove plants. I. Lipids and carbohydrates in propagules of ten mangrove species of China. Mokuzai Gakkaishi 43:875–881Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • T. Meziane
    • 1
    • 5
    Email author
  • S. Y. Lee
    • 2
  • P. L. Mfilinge
    • 3
  • P. K. S. Shin
    • 4
  • M. H. W. Lam
    • 4
  • M. Tsuchiya
    • 3
  1. 1.Centre for Aquatic Processes and PollutionGriffith University PMB 50 Gold CoastAustralia
  2. 2.Centre for Aquatic Processes and Pollution and School of Environmental and Applied ScienceGriffith UniversityPMB 50 Gold CoastAustralia
  3. 3.Laboratory of Ecology and Systematics, Faculty of ScienceUniversity of the Ryukyus NishiharaJapan
  4. 4.Department of Biology and ChemistryCity University of Hong Kong KowloonChina
  5. 5.UMR-CNRS 5178, Biologie des Organismes Marins et Ecosystèmes, Département Milieux et Peuplements Aquatiques, MNHNParis cedex 05France

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