Advertisement

Marine Biology

, Volume 150, Issue 3, pp 455–462 | Cite as

Cloning and expression of a TCTP homolog from the ovaries of banana prawn

  • Wiriya Loongyai
  • Amornrat Phongdara
  • Wilaiwan Chotigeat
Research Article

Abstract

Ovarian development in prawns is not well understood. The up-regulated prawn ovarian translationally controlled tumor protein (Pmer-TCTP) of the banana prawn (Penaeus merguiensis) was quantitated by real-time PCR. The results showed that the gene has a significantly increased expression (< 0.05) in the early stage of matured ovaries (stage 1) whereas Pmer-TCTP transcripts were not significantly increased at the later stage (stages 2 and 3) when compared to the non-matured ovaries. This result corresponds to the result of the western blot analysis when compared with the amounts of native Pmer-TCTP protein from different vitellogenic stages. The result of the western blot analysis indicated that the native Pmer-TCTP is present in higher concentration at the early stage and the molecular mass of the native Pmer-TCTP was about 26 kDa. The deduced amino acid sequence of Pmer-recombinant TCTP was found to be 98% (4e-91) similar to Penaeus monodon TCTP (Pm-TCTP), 44% (3e−27) to human TCTP and 42% (2e−25) to mouse TCTP. The expressed Pmer-recombinant TCTP in Escherichia coli produced a protein of about 19.2 kDa on SDS-PAGE and both the native Pmer-TCTP and Pmer-recombinant TCTP interacted with elongation factor 1 alpha.

Keywords

Translationally Control Tumor Protein Suppressive Subtractive Hybridization Control Tumor Protein Vitellogenic Stage Myosin Light Chain Kinase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are most grateful to the Trang Coastal Aquaculture Station, Trang province, Thailand, for the prawn samples, Penaeus merguiensis. This work was supported by the Royal Golden Jubilee Graduate Program from the Thailand Research Fund (TRF) for Wiriya Loongyai (4.C.PS/45/G.1) as well as the Excellent Scholarship in Biochemistry of Prince of Songkla University. We thank Dr. Brian Hodgson, Faculty of Science, Prince of Songkla University, for reading the manuscript and for valuable comments.

References

  1. Babiychuk BE, Babiychuk VS, Sobieszek A (1995) Modulation of smooth muscle myosin light chain kinase activity by Ca2+/calmodulin-dependent, oligomeric-type modifications. Biochemistry 34:6366–6372CrossRefGoogle Scholar
  2. Bangrak P, Graidist P, Chotigeat W, Phongdara A (2004) Molecular cloning and expression of a mammalian homologue of a translationally controlled tumor protein (TCTP) gene from Penaeus monodon shrimp. J Biotechnol 108:219–226CrossRefGoogle Scholar
  3. Baudet C, Perret E, Delpech B, Kaghad M, Brachet P, Wion D, Caput D (1998) Differentially expressed genes in C6.9 glioma cells during vitamin D-induced cell death program. Cell Death Differ 5:116–125CrossRefGoogle Scholar
  4. Bohm H, Benndorf R, Gaestel M, Gross B, Nurnberg P, Kraft R, Otto A, Bielka H (1989) The growth-related protein P23 of the Ehrlich ascites tumor: translational control, cloning and primary structure. Biochem Int 19(2):277–86PubMedGoogle Scholar
  5. Bommer U-A, Lazaris-Karatzas A, De Benedetti A, Nurnberg P, Benndorf R, Bielka H, Sonenberg N (1994) Translational regulation of the mammalian growth-related protein P23: involvement of eIF-4E. Cell Mol Biol Res 40(7–8):633–641PubMedGoogle Scholar
  6. Bommer U-A, Thiele B-J (2004) The translationally controlled tumour protein (TCTP). Int J Biochem Cell Biol 36(3):379–385CrossRefGoogle Scholar
  7. Bonnet C, Perret E, Dumont X, Picard A, Caput D, Lenaers G (2000) Identification and transcription control of fission yeast genes repressed by an ammonium starvation growth arrest. Yeast 16:23–33CrossRefGoogle Scholar
  8. Gachet Y, Tournier S, Lee M, Karatzas AL, Poulton T, Bommer UA (1999) The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle. J Cell Sci 112:1257–1271PubMedGoogle Scholar
  9. Langdon JM, Vonakis BM, Macdonal SM (2004) Identification of the interaction between the human recombinant hitamine releasing factor/translationally controlled tumor protein and elongation factor-1 delta (also known as elongation factor-1-B). Biochim Biophys Acta 1688:232–236CrossRefGoogle Scholar
  10. Stierum R, Gaspari M, Dommels Y, Ouatas T, Pluk H, Jespersen S, Vogels J, Verhoeckx K, Groten J, Ommen BV (2003) Proteome analysis reveals novel proteins associated with the proliferation and differentiation of the colorectal cancer cell line Caco-2. Biochim Biophys Acta 1650:73–91CrossRefGoogle Scholar
  11. Sturzenbaum SR, Kille P, Morgan AJ (1998) Identification of heavy metal induced changes in the expression patterns of the translationally controlled tumour protein (TCTP) in the earthworm Lumbricus rubellus1. Biochim Biophys Acta 1398(3):294–304CrossRefGoogle Scholar
  12. Thiele H, Berger M, Skalweit A, Theiele BJ (2000) Expression of the gene and processed pseudogenes encoding the human and rabbit translationally controlled tumour protein (TCTP). Eur J Biochem 267:5470–5481CrossRefGoogle Scholar
  13. Thomas NG, Luther H (1981) Transcriptional and translational control of cytoplasmic proteins after serum stimulation of quiescent Swiss 3T3 cells. Proc Natl Acad Sci USA 78(9):5712–5716CrossRefGoogle Scholar
  14. Tuynder M, Susini L, Prieur S, Besse S, Fiucci G, Amson R, Telerman A (2002) Biological models and genes of tumor reversion: cellular reprogramming through tpt1_TCTP and SIAH-1. Proc Natl Acad Sci USA 99(23):14976–14981CrossRefGoogle Scholar
  15. Woo H, Hawes MC (1997) Cloning of genes whose expression is correlated with mitosis and localized in dividing cells in root caps of Pisum sativum L. Plant Mol Biol 35:1045–1051CrossRefGoogle Scholar
  16. Yan L, Fei K, Bridge D, Sarras MP (2000) A cnidarian homologue of translationally controlled tumor protein (P23/TCTP). Dev Genes Evol 210:507–511CrossRefGoogle Scholar
  17. Yoon T, Jung J, Kim M, Lee KM, Choi EC, Lee K (2000) Identification of the self-interaction of rat TCTP/IgE dependent histamine-releasing factor using yeast two-hybrid system. Arch Biochem Biophys 384:379–382CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Wiriya Loongyai
    • 1
  • Amornrat Phongdara
    • 1
  • Wilaiwan Chotigeat
    • 1
  1. 1.Department of Biochemistry, Faculty of Science Prince of Songkla UniversitySongklaThailand

Personalised recommendations